In vitro plant regeneration from cotyledonary nodes of Withania somnifera (L.) Dunal and assessment of clonal fidelity using RAPD and ISSR markers

• Autorzy: Nayak S.A. , Kumar S. , Satapathy K. , Moharana A. , Behera B. , Barik D. P. , Acharya L. , Mohapatra P. K. , Jena P. K. , Naik S. K.
• Języki publikacji: EN

Abstrakty

EN

An efficient large-scale clonal propagation protocol has been described for Withania somnifera (L.) Dunal, a valuable medicinal plant, using cotyledonary nodes derived from axenic seedlings. Murashige and Skoog’s (Physiol Plant 15:473–497, 1962) (MS) medium supplemented with 1.0 mg l-1 N6-benzyladenine (BA) was found to be optimum for production of multiple shoots (100 % shoot proliferation frequency and 16.93 shoots per explant). Successive shoot cultures were established by repeatedly sub-culturing the original cotyledonary node on a fresh medium after each harvest of newly formed shoots. Multiple shoot proliferation was also achieved from nodal segments derived from in vitro raised shoots on MS medium augmented with 1.0 mg l-1 BA. Regenerated shoots were best rooted (95.2 %, 38.7 roots per shoot) in half-strength MS medium supplemented with 1.0 mg l-1 indole-3-butyric acid. The plantlets were successfully acclimated and established in soil. Random amplified polymorphic DNA and inter-simple sequence repeats analysis revealed a homogeneous amplification profile for all micropropagated plants analyzed validating the genetic fidelity of the in vitro regenerated plants.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2013
• Tom: 35
• Numer: 01
• Opis fizyczny: p.195-203,fig.,ref.

Twórcy

autor

Nayak S.A.

• Department of Botany, Ravenshaw University, Cuttack 753 003, Odisha, India

autor

Kumar S.

• Department of Botany, Ravenshaw University, Cuttack 753 003, Odisha, India

autor

Satapathy K.

autor

Moharana A.

• Department of Botany, Ravenshaw University, Cuttack 753 003, Odisha, India

autor

Behera B.

• Department of Botany, Ravenshaw University, Cuttack 753 003, Odisha, India

autor

Barik D. P.

• Department of Botany, Ravenshaw University, Cuttack 753 003, Odisha, India

autor

Acharya L.

autor

Mohapatra P. K.

• Department of Botany, Ravenshaw University, Cuttack 753 003, Odisha, India

autor

Jena P. K.

• Department of Botany, Ravenshaw University, Cuttack 753 003, Odisha, India

autor

Naik S. K.

• Department of Botany, Ravenshaw University, Cuttack 753 003, Odisha, India

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