PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2014 | 36 | 05 |

Tytuł artykułu

Phytotoxic activity of Cachrys pungens Jan, a mediterranean species: separation, identification and quantification of potential allelochemicals

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
In continuous research for bioactive compounds obtained from plants to use for weed control in sustainable agriculture, the aerial parts of Cachrys pungens Jan (Umbelliferae) were extracted with methanol and then fractionated using hexane, chloroform (CHCl₃) and ethyl acetate (AcOEt). The potential phytotoxicity of total methanolic extract and each fraction was assayed in vitro on seed germination and root elongation of lettuce (Lactuca sativa L.) and the most active fractions were assayed on three of the most common weeds (Lolium perenne, Amaranthus retroflexus, Echinochloa crus-galli). Non linear regression that allowed to obtain the ED₅₀ index for both physiological processes was applied. The fraction bioassays indicated the following hierarchy of phytotoxicity for both processes: CHCl₃ ≥ AcOEt > hexane. Moreover, in the present work was chemically characterized for the first time (through HPTLC) the polar fraction of this species pointing out the high presence of flavonoids and phenolic acids. In particular six of them have been chemically characterized and quantified (naringin, quercetin, catechin, caffeic acid, ferulic acid and gallic acid). These results make C. pungens Jan a potential source of natural compounds employable for an eco-friendly agriculture.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

36

Numer

05

Opis fizyczny

p.1071-1083,fig.,ref.

Twórcy

autor
  • Dipartimento di AGRARIA, Facolta di Agraria-Salita Melissari, Universita degli Studi "Mediterranea" di Reggio Calabria, 89124 Reggio Calabria, RC, Italy
autor
  • Dipartimento di Farmacia e Scienze della Salute e della Nutrizione, Universita della Calabria, 87030 Rende, CS, Italy
autor
  • Dipartimento di AGRARIA, Facolta di Agraria-Salita Melissari, Universita degli Studi "Mediterranea" di Reggio Calabria, 89124 Reggio Calabria, RC, Italy
autor
  • Dipartimento di AGRARIA, Facolta di Agraria-Salita Melissari, Universita degli Studi "Mediterranea" di Reggio Calabria, 89124 Reggio Calabria, RC, Italy
autor
  • Dipartimento di Farmacia e Scienze della Salute e della Nutrizione, Universita della Calabria, 87030 Rende, CS, Italy
  • Dipartimento di AGRARIA, Facolta di Agraria-Salita Melissari, Universita degli Studi "Mediterranea" di Reggio Calabria, 89124 Reggio Calabria, RC, Italy

Bibliografia

  • Abad MJ, Heras BDL, Silvan AM, Pascual R, Bermejo P, Rodriguez B, Villar AM (2001) Effects of furocoumarins from Cachrys trifida on some macrophage functions. J Pharm Pharmacol 53(8):1163–1168
  • Abenavoli MR, Cacco G, Sorgonà A, Marabottini R, Paolacci AR, Ciaffi M, Badiani M (2006) The inhibitory effects of coumarin on the germination of durum wheat (Triticum turgidum ssp. durum, CV. Simeto) seeds. J Chem Ecol 32(2):489–506
  • Ahire ML, Walunj PR, Kishor PBK, Nikam TD (2013) Effect of sodium chloride-induced stress on growth, proline, glycine betaine accumulation, antioxidative defence and bacoside A content in in vitro regenerated shoots of Bacopa monnieri (L.) Pennell. Acta Physiol Plant 35:1943–1953
  • Aliotta G, Cafiero G, Fiorentino A, Strumia S (1993) Inhibition of radish germination and root growth by coumarin and phenyl-propanoids. J Chem Ecol 19(2):175–183
  • Aliotta G, Cafiero G, De Feo V, Sacchi R (1994) Potential allelochemicals from Ruta graveolens L. and their action on radish seeds. J Chem Ecol 20(11):2761–2775
  • An M, Haig T, Pratley JE (2000) Phytotoxicity of Vulpia residues: II. Separation, identification, and quantitation of allelochemicals from Vulpia myuros. J Chem Ecol 26:1465–1476
  • Anjum T, Bajwa R (2010) Isolation of bioactive allelochemicals from sunflower (variety Suncross-42) through fractionation-guided bioassays. Nat Prod Res 24(18):1783–1788
  • Araniti F, Lupini A, Sorgonà A, Conforti F, Marrelli M, Statti GA, Menichini F, Abenavoli MR (2012a) Allelopathic potential of Artemisia arborescens: isolation, identification and quantification of phytotoxic compounds through fractionation-guided bioassays. Nat Prod Res 27(10):880–887
  • Araniti F, Sorgonà A, Lupini A, Abenavoli MR (2012b) Screening of mediterranean wild plant species for allelopathic activity and their use as bio-herbicides. Allelopathy J 29(1):107–124
  • Araniti F, Lupini A, Mercati F, Statti GA, Abenavoli MR (2013) Calamintha nepeta L.(Savi) as source of phytotoxic compounds: bio-guided fractionation in identifying biological active molecules. Acta Physiol Plant 35(6):1979–1988
  • Baghestani A, Lemieux C, Leroux GD, Baziramakenga R, Simard RR (1999) Determination of allelochemicals in spring cereal cultivars of different competitiveness. Weed Sci 47:498–504
  • Bais HP, Walker TS, Kennan AJ, Stermitz FR, Vivanco JM (2003) Structure-dependent phytotoxicity of catechins and other flavonoids: flavonoid conversions by cell-free protein extracts of Centaurea maculosa (spotted knapweed) roots. J Agric Food Chem 51(4):897–901
  • Bubna GA, Lima RB, Zanardo DYL, Dos Santos WD, Ferrarese MDLL, Ferrarese-Filho O (2011) Exogenous caffeic acid inhibits the growth and enhances the lignification of the roots of soybean (Glycine max). J Plant Physiol 168(14):1627–1633
  • Chaves N, Escudero JC (1999) Variation of flavonoid synthesis induced by ecological factors. Principles and Practices of Plant Ecology, Allelochemical Interactions, 267–285
  • Chiapusio G, Sanchez AM, Reigosa MJ, Gonzalez L, Pellissier F (1997) Do germination indices adequately reflect allelochemical effects on the germination process? J Chem Ecol 23(11):2445–2453
  • Chon SU, Choi SK, Jung S, Jang HG, Pyo BS, Kim SM (2002) Effects of alfalfa leaf extracts and phenolic allelochemicals on early seedling growth and root morphology of alfalfa and barnyard grass. Crop Prot 21(10):1077–1082
  • Chung IM, Miller DA (1995) Natural herbicide potential of alfalfa residue on selected weed species. Agron J 87(5):920–925
  • Conforti F, Sosa S, Marrelli M, Menichini F, Statti GA, Uzunov D, Tubaro A, Menichini F (2009) The protective ability of mediterranean dietary plants against the oxidative damage: the role of radical oxygen species in inflammation and the polyphenol, flavonoid and sterol contents. Food Chem 112:587–594
  • De Martino L, Mencherini T, Mancini E, Aquino RP, De Almeida LFR, De Feo V (2012) In vitro phytotoxicity and antioxidant activity of selected flavonoids. Int J Mol Sci 13(5):5406–5419
  • Devi SR (1992) Effects of ferulic acid on growth and hydrolytic enzyme activities of germinating maize seeds. J Chem Ecol 18:1981–1990
  • Djurdjevic L, Dinic A, Pavlovic P, Mitrovic M, Karadzic B, Tesevic V (2004) Allelopathic potential of Allium ursinum L. Biochem Syst Ecol 32:533–544
  • Grande M, Aguado MT, Mancheño B, Piera F (1986) Coumarins and ferulol esters from Cachrys sicula. Phytochem 25(2):505–507
  • Hashim MS, Devi KS (2003) Insecticidal action of the polyphenolic rich fractions from the stem bark of Streblus asper on Dysdercus cingulatus. Fitoterapia 74(7):670–676
  • Hassan S, Mathesius U (2012) The role of flavonoids in root-rhizosphere signalling: opportunities and challenges for improving plant-microbe interactions. J Exp Bot 63(9):3429–3444
  • Hoult JRS, Paya M (1996) Pharmacological and biochemical actions of simple coumarins: natural products with therapeutic potential. Gen Pharmacol: Vasc Syst 27(4):713–722
  • Ignatèva NS, Vandyshev VV, Pimenov MG (1972) Coumarins from the roots of Cachrys pubescens. Chem Nat Compd 8(3):381
  • Imatomi M, Novaes P, Matos AP, Gualtieri SC, Molinillo JM, Lacret R, Varela RM, Macías FA (2013) Phytotoxic effect of bioactive compounds isolated from Myrcia tomentosa (Myrtaceae) leaves. Biochem Syst Ecol 46:29–35
  • Inderjit, Dakshini KMM (1995) Quercetin and quercitrin from Pluchea lanceolata and their effect on growth of asparagus bean. In: Inderjit, Dakshini KMM, Einhellig FA (eds) Allelopathy: organisms, processes, and applications. ACS Symposium Series, Washington, DC, pp 86–93
  • Kaur H, Inderjit B, Kaushik S (2005) Cellular evidence of allelopathic interference of benzoic acid to mustard (Brassica juncea L.) seedling growth. Plant Physiol Biochem 43:77–81
  • Kikowska M, Budzianowski J, Krawczyk A, Thiem B (2012) Accumulation of rosmarinic, chlorogenic and caffeic acids in in vitro cultures of Eryngium planum L. Acta Physiol Plant 34:2425–2433
  • Kil BS, Yun KW (1992) Allelopathic effects of water extracts of Artemisia princeps var. orientalis on selected plant species. J Chem Ecol 18(1):39–51
  • Macias FA, Molinillo JM, Varela RM, Galindo JC (2007) Allelopathy: a natural alternative for weed control. Pest Manag Sci 63(4):327–348
  • Menichini G, Alfano C, Provenzano E, Marrelli M, Statti GA, Menichini F, Conforti F (2012) Cachrys pungens Jan inhibits human melanoma cell proliferation through photo-induced cytotoxic activity. Cell Proliferat 45(1):39–47
  • Nicoletti M (2011) HPTLC fingerprint: a modern approach for the analytical determination of botanicals. Rev Bras Farmacogn 21:818–823
  • Nielsen OK, Ritz C, Streibeg JC (2004) Nonlinear mixed-model regression to analyze herbicide dose: response relationships. Weed Technol 18:30–37
  • Patterson DT (1981) Effects of allelopathic chemicals on growth and physiological response of soybean (Glycine max). Weed Sci 29:53–58
  • Petersen J, Belz R, Walker F, Hurle K (2001) Weed suppression by release of isothiocyanates from turnip-rape mulch. Agron J 93:37
  • Quettier-Deleu C, Gressier B, Vasseur J, Dine T, Brunet C, Luyckx M, Cazin M, Cazin J-C, Bailleul F, Trotin F (2000) Phenolic compounds and antioxidant activities of buckwheat (Fagopyrum esculentum Moench) hulls and flour. J Ethnopharmacol 72(1):35–42
  • Rashmin BP, Mrunali PP, Bharat GB (2011) Experimental aspects and implementation of HPTLC. In: Srivastava MM (ed) High-performance thin-layer chromatography (HPTLC). Springer, Berlin, pp 41–54
  • Reigosa MJ, Pazos-Malvido E (2007) Phytotoxic effects of 21 plant secondary metabolites on Arabidopsis thaliana germination and root growth. J Chem Ecol 33(7):1456–1466
  • Rice EL (1984) Allelopathy, 2nd edn. Academic press Inc., Orlando Rizvi SJH, Haque H, Singh VK, Rizvi V (1992) A discipline called allelopathy. In: Rizvi SJH (ed) Allelopathy. Basic and applied aspects, Chapman 6 Hall, London 1–8
  • Sasikumar K, Vijayalakshmi C, Parthiban KT (2002) Allelopathic effects of Eucalyptus on blackgram (Phaseolus mungo L.). Allelopathy J 9:205–214
  • Selmar D, Kleinwächter M (2013) Stress enhances the synthesis of secondary plant products: the impact of stress-related over-reduction on the accumulation of natural products. Plant Cell Physiol 54(6):817–826
  • Tutin TG, Heywood VH, Valentine DH, Burges NA, Moore DM, Walters SM (1968) Flora Europaea. Cambridge University Press, London
  • Weir TL, Park SW, Vivanco JM (2004) Biochemical and physiological mechanisms mediated by allelochemicals. Curr Opin Plant Biol 7(4):472–479
  • Weston LA, Mathesius U (2013) Flavonoids: their structure, biosynthesis and role in the rhizosphere, including allelopathy. J Chem Ecol 39(2):283–297
  • Zhu H, Mallik AU (1994) Interactions between kalmia and black spruce: isolation and identification of allelopathic compounds. J Chem Ecol 20:407–421
  • Zohary M (1972) Flora palaestina. Goldberg, Jerusalem

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-4e82e5ba-85d0-4daf-868a-d5581e733643
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.