Role of polyamines in gastroprotection induced by epidermal growth factor

• Autorzy: Brzozowski T. , Drozdowicz D. , Majka J. , Polonczyk-Pytko J. , Konturek S. J.
• Języki publikacji: EN

Abstrakty

EN

Polyamines have been shown to stimulate cellular growth and differentiation, though their role in the prevention of acute gastric lesion induced by various noxious agents has been little studied. Epidermal growth factor (EGF) exhibits gastroprotective and ulcer healing properties due to its potent mitogenic and growth promoting action. This study was designed to compare the gastroprotective effects of spermine and EGF against gastric damage induced by absolute ethanol, acidified aspirin and stress and to determine the role of endogenous polyamines in EGF-induced gast- troprotection. Spermine and EGF significantly reduced the lesions induced by all three ulcerogens. Oral administration of spermine or subcutaneous infusion of EGF in 24 h fasted rats with chronic gastric fistula resulted in similar inhibition of gastric acid and pepsin secretion. Pretreatment with difluoromethylornithine (DFMO), an irreversible inhibitor of ornithine decarboxylase (ODC), a hey enzyme in the biosynthesis of polyamines, did not affect ethanol lesions, but reversed the protective effect EGF but not spermine, against ethanol. This finding indicates that polyamines mediate, at least in part, EGF-induced gastroprotection. In tests with oral administration of aminoguanidine that is known to suppress the activity of diamino-oxidase (DAO) and to inhibit the degradation of polyamines, EGF showed a markedly enhanced gastroprotective activity against ethanol damage. Since indomethacin failed to affect the gastroprotective effects of spermine and EOF and neither of these agents influenced the mucosal generation of PGE₂ in intact or injured gastric mucosa, we conclude that prostaglandins are not the major factors in spermine- and EGF-induced gastroprotection. This study demonstrates that polyamines are highly effective against gastric lesions induced by various ulcerogens and that they act as primary mediators of EGF-induced gastroprotection.

Słowa kluczowe

EN

gastroprotection; polyamine; epidermal growth factor; prostaglandin; ornithine decarboxylase; aminoguanidine; gastric lesion; ulcer healing; spermine; difluoromethylornithine

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology
• Rocznik: 1991
• Tom: 42
• Numer: 2
• Opis fizyczny: p.181-193,fig.,ref.

Twórcy

autor

Brzozowski T.

• Institute of Physiology, Academy of Medicine, Grzgorzecka 16, 31-531 Krakow, Poland

autor

Drozdowicz D.

• Institute of Physiology, Academy of Medicine, Grzgorzecka 16, 31-531 Krakow, Poland

autor

Majka J.

• Institute of Physiology, Academy of Medicine, Grzgorzecka 16, 31-531 Krakow, Poland

autor

Polonczyk-Pytko J.

• Institute of Physiology, Academy of Medicine, Grzgorzecka 16, 31-531 Krakow, Poland

autor

Konturek S. J.

• Institute of Physiology, Academy of Medicine, Grzgorzecka 16, 31-531 Krakow, Poland

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