Examination of the microscopic imaging of neural stem cells for counting a number of cells

• Autorzy: Korzrynska A. , Dobrowolska E. , Zychowicz M. , Hoser P.
• Języki publikacji: EN

Abstrakty

EN

The goal of the investigation is to examine cells imaging with the brightfi eld and confocal microscopic imaging using two techniques: with and without fl uorescence. The investigations are done as an introducing part of the long term goal to develop methods of monitoring a number of cells in culture in time. Several types of events in the monitored cellsí culture, e. g. cellís division and death, cellsí fusion and its changes in shape and/or position should be detected and quantifi ed. Because of immanent features of each microscopic technique some of events are easier to be observed in one of the microscopic techniques than in others. Other events are detectable in all microscopic imaging. To detect them, the area covered by the cells in the image should be selected in the fi rst step of the image processing. So the method of cellsí area detection, described by Korzynska and Iwanowski (2008) [In: Pietka, Kawa (Eds), Information Technique in Biomedicine, ASC 47, pp. 365ñ376], is applied to all tested types of the microscopic images of HUCB-NSC transfected by GFP (from Medical Research Center PAS) and the results of area detection are examined and compared. It was found, that the proposed method detects cellsí area more precisely in the fl uorescent images, than in the brightfi eld or confocal microscopy. Comparing results of cellsí area detection of the fl uorescent image of confocal microscopy with the integrated fl uorescent signal in epifl uorescent images of conventional optical microscopy the better result of detection cells in the image plane were observed for fl attened, large cells in confocal images while for rounded, convergent ones for the brightfi eld microscopic techniques.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2009
• Tom: 69
• Numer: 1
• Opis fizyczny: p.106

Twórcy

autor

Korzrynska A.

• Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland

autor

Dobrowolska E.

• Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland

autor

Zychowicz M.

• NeuroRepair Department, Mossakowski Medical Research Centre Polish Academy of Sciences, Warsaw Poland

autor

Hoser P.

• Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland