Thermophilic potentially pathogenic amoebae isolated from natural water bodies in Poland and their molecular characterization

• Autorzy: Adamska M. , Leonska-Duniec A. , Lanocha N. , Skotarczak B.
• Języki publikacji: EN

Abstrakty

EN

The free-living amoebae (FLA) may live in the environment and also within other organisms as parasites and then they are called amphizoic. They are potentially pathogenic for humans and animals and are found in water that is a source of infection. The aim of this study was molecular detection and identification of these FLA in natural water bodies in North-Western Poland to evaluate the risk of the pathogenic amoebae infections. We examined surface water samples collected from 50 sites and first, the tolerance thermic test was performed in order to select thermophilic, potentially pathogenic strains. For molecular identification of FLA, regions of 18S rDNA, 16S rDNA and intergenic spacers were amplified. Acanthamoeba T4 and T16 genotypes of 18S rDNA gene and 18S rDNA of H. vermiformis were detected. We identified two variants of Acanthamoeba T4 genotype, two variants of Acanthamoeba T16 genotype and one variant of H. vermiformis. Identification of the T16 genotype and H. vermiformis in water was for the first time in Poland. Additionally, we made attempts to adapt the RLB method for detection and differentiation of FLA species and strains. PCR seems to be more sensitive than RLB hybridization, though.

Słowa kluczowe

EN

free-living amoeba; Acanthamoeba; T4 genotype; T16 genotype; genotyping; Hartmannella vermiformis; water body; North-Western Poland; pathogenic amoeba; infection; thermophilic strain; molecular characteristics

• Wydawca: -
• Czasopismo: Acta Parasitologica
• Rocznik: 2014
• Tom: 59
• Numer: 3
• Opis fizyczny: p.433-441,ref.

Twórcy

autor

Adamska M.

• Department of Genetics, Szczecin University, Felczaka 3c, 71-412 Szczecin, Poland

autor

Leonska-Duniec A.

• Department of Genetics, Szczecin University, Felczaka 3c, 71-412 Szczecin, Poland

autor

Lanocha N.

• Chair and Department of Biology and Medical Parasitology, Pomeranian Medical University, Szczecin, Poland

autor

Skotarczak B.

• Department of Genetics, Szczecin University, Felczaka 3c, 71-412 Szczecin, Poland

Bibliografia

• Alsam S., Kim K.S., Stins M., Rivas A.O., Sissons J., Khan N.A. 2003. Acanthamoeba interactions with human brain microvascular endothelial cells. Microbial Pathogenesis, 35, 235–241. DOI:10.1016/j.micpath.2003.07.001.
• Booton G.C., Kelly D.J., Chu Y.W., Seal D.V., Houang E., Lam D.S., Byers T.J., Fuerst P.A. 2002. 18S ribosomal DNA typing and tracking of Acanthamoeba species isolates from corneal scrape specimens, contact lenses, lens cases, and home water supplies of Acanthamoeba keratitis patients in Hong Kong. Journal of Clinical Microbiology, 40, 1621–1625. DOI:10.1128/JCM.40.5.1621-1625.2002.
• Booton G.C., Visvesvara G.S., Byers T.J., Kelly D.J., Fuerst P.A. 2005. Identification and distribution of Acanthamoeba species genotypes associated with nonkeratitis infections. Journal of Clinical Microbiology, 43, 1689–1693. DOI: 10.1128/JCM43.4.1689-1693.2005.
• Brown M.W., Spiegel F.W., Silberman J.D. 2007. Amoeba at attention: phylogenetic affinity of Sappinia pedata. Journal of Eukaryotic Microbiology, 54, 511–519. DOI: 10.1111/j.1550-7408.2007.00292.x.
• Corsaro D., Venditti D. 2010. Phylogenetic evidence for a new genotype of Acanthamoeba (Amoebozoa, Acanthamoebida). Parasitology Research, 107, 233–238. DOI: 10.1007/s00436-010-1870-6.
• Corsaro D., Venditti D. 2011. More Acanthamoeba genotypes: limits to the use of rDNA fragments to describe new genotypes. Acta Protozoologica, 50, 49–54.
• Da Rocha-Azevedo B., Tanowitz H.B., Marciano-Cabral F. 2009. Diagnosis of infections caused by pathogenic free-living amoebae. Interdisciplinary Perspectives on Infectious Diseases, 2009,1–14. DOI:10.1155/2009/251406.
• De Jonckheere J.F. 1980. Growth characteristics, cytopathic effect in cell culture, and virulence in mice of 36 type strains belonging to 19 different Acanthamoeba spp. Applied and Environmental Microbiology, 39, 681–685.
• Derda M., Hadaś E., Wojtkowiak-Giera A., Wojt W.J., Cholewinski M., Skrzypczak L. 2013. The occurrence of free-living amoebae in fountains. Probl. Hig. Epidemiol., 94, 147–150.
• Dyková I., Kostka M., Wortberg F., Nardy E., Pecková H. 2010. New data on etiology of nodular gill disease in rainbow trout, Oncorhynchus mykiss. Folia Parasitologica (Praha), 57, 157–163.
• Dyková I., Pindová Z., Fiala I., Dvoráková H., Machácková B. 2005. Fish-isolated strains of Hartmannella vermiformis page, 1967: morphology, phylogeny and molecular diagnosis of the species in tissue lesions. Folia Parasitologica (Praha), 52, 295–303.
• Gast R.J. 2001. Development of an Acanthamoeba — specific reverse dot — blot and the discovery of a new ribotype. Journal of Eukaryotic Microbiology, 48, 609–615.
• Gast R.J., Ledee D.R., Fuerst P.A., Byers T.J. 1996. Subgenus systematics of Acanthamoeba: four nuclear 18S rDNA sequence types. Journal of Eukaryotic Microbiology, 43, 498–504.
• Gianinazzi C., Schild M., Zumkehr B., Wüthrich F., Nüesch I., Ryter R., Schürch N., Gottstein B., Müller N. 2010. Screening of Swiss hot spring resorts for potentially pathogenic free-living amoebae. Experimental Parasitology, 126, 45–53. DOI:10.1016/j.exppara.2009.12.008.
• Górnik K., Kuźna-Grygiel W. 2004. Presence of virulent strains of amphizoic amoebae in swimming pools of the city of Szczecin. Annals of Agricultural and Environmental Medicine, 11, 233–236.
• Gubbels J.M., de Vos A.P., van der Weide M., Viseras J., Schouls L.M., de Vries E., Jongejan F. 1999. Simultaneus detection of bovine Theileria and Babesia species by reverse line blot hybridization. Journal of Clinical Microbiology, 37, 1782–1789.
• Hewett M.K., Robinson B.S., Monis P.T., Saint C.P. 2003. Identification of a new Acanthamoeba 18S rRNA gene sequence type, corresponding to the species Acanthamoeba jacobsi Sawyer, Nerad and Visvesvara, 1992 (Lobosea: Acanthamoebidae). Acta Protozoologica, 42, 325–329.
• Horn M., Fritsche T.R., Gautom R.K., Schleifer K.H., Wagner M. 1999. Novel bacterial endosymbionts of Acanthamoeba spp. related to the Paramecium caudatum symbiont Caedibacter caryophilus. Environmental Microbiology, 1, 357–367. DOI: 10.1046/j.1462-2920.1999.00045.x.
• Hsu B.M., Lin C.L., Shih F.C. 2009. Survey of pathogenic free-living amoebae and Legionella spp. in mud spring recreation area. Water Research, 43, 2817–2828. DOI: 10.1016/j.watres.2009.04.002.
• Khan N.A. 2006. Acanthamoeba: biology and increasing importance in human health. FEMS Microbiology Reviews, 30, 564–595. DOI: 10.1111/j.1574-6976.2006.00023.x.
• Khan N.A., Jarroll E.L., Paget T.A. 2002. Molecular and physiological differentiation between pathogenic and nonpathogenic Acanthamoeba. Current Microbiology, 45, 197–202. DOI: 10.1007/s00284-001-0108-3.
• Krometis L.A.H., Characklis G.W., Sobsey M.D. 2009. Identification of particle size classes inhibiting protozoan recovery from surface water samples via U.S. Environmental Protection Agency Method 1623. Applied and Environmental Microbiology, 75, 6619–6621. DOI: 10.1128/AEM.01136-09.
• Kuiper M.W., Valster R.M., Wullings B.A., Boonstra H., Smidt H., van der Kooij D. 2006. Quantitative detection of the free-living amoeba Hartmannella vermiformis in surface water by using real-time PCR. Applied and Environmental Microbiology, 72, 5750–5756. DOI: 10.1128/AEM.00085-06.
• Landell M.F., Salton J., Caumo K., Broetto L., Rott M.B. 2013. Isolation and genotyping of free-living environmental isolates of Acanthamoeba spp. from bromeliads in Soutern Brazil. Experimental Parasitology 134: 290–294. DOI: 10.1016/j.exppara.2013.03.028.
• Lares-Villa F., Hernández-Peña C. 2010. Concentration of Naegleria fowleri in natural waters used for recreational purposes in Sonora, Mexico (November 2007–October 2008). Experimental Parasitology, 126, 33–36. DOI: 10.1016/j.exppara.2010.04.011.
• Lorenzo-Morales J., Monteverde-Miranda C.A., Jiménez C., Tejedor M.L., Valladares B., Ortega-Rivas A. 2005. Evaluation of Acanthamoeba isolates from environmental sources in Tenerife, Canary Islands, Spain. Annals of Agricultural and Environmental Medicine, 12, 233–236.
• Lanocha N., Kosik-Bogacka D., Maciejewska A., Sawczuk M., Wilk A., Kuzna-Grygiel W. 2009. The occurrence Acanthamoeba (free living amoeba) in environmental and respiratory samples in Poland. Acta Protozoologica, 48, 271–279.
• Nagyová V., Nagy A., Janeček Š., Timko J. 2010. Morphological, physiological, molecular and phylogenetic characterization of new environmental isolates of Acanthamoeba spp. from the region of Bratislava, Slovakia. Biologia, 65, 81–91. DOI: 10.2478/s11756-009-0217-1.
• Nuprasert W., Putaporntip C., Pariyakanok L., Jongwutiwes S. 2010. Identification of a novel T17 genotype of Acanthamoeba from environmental isolates and T10 genotype causing keratitis in Thailand. Journal of Clinical Microbiology, 48, 4636–4640. DOI: 10.1128/JCM.01090-10.
• Qvarnstrom Y., da Silva A.J., Schuster F.L., Gelman B.B., Visvesvara G.S. 2009. Molecular confirmation of Sappinia pedata as a causative agent of amoebic encephalitis. Journal of Infectious Diseases, 199, 1139–1142. DOI: 10.1086/597473.
• Qvarnstrom Y., Nerad T.A., Visvesvara G.S. 2013. Characterisation of a new pathogenic Acanthamoeba species, A. byersi n. sp., isolated from a human with fatal amoebic encephalitis. Journal of Eucaryotic Microbiology, 60, 626–633. DOI:10.1111/jeu12069.
• Schroeder J.M., Booton G.C., Hay J., Niszl I.A., Seal D.V., Markus M.B., Fuerst P.A., Byers T.J. 2001. Use of subgenic 18S ribosomal DNA PCR and sequencing for genus and genotype identification of Acanthamoebae from humans with keratitis and from sewage sludge. Journal of Clinical Microbiology, 39(5), 1903–1911. DOI:10.1128/JCM.39.5.1903-1911.2001.
• Schuster F.L., Visvesvara G.S. 2004. Free-living amoebae as opportunistic and non-opportunistic pathogens of humans and animals. International Journal of Parasitology, 34, 1001–1027. DOI:10.1016/j.ijpara.2004.06.004
• Siddiqui R., Khan N.A. 2012. Biology and pathogenesis of Acanthamoeba. Parasites and Vectors, 5, 6. DOI: 10.1186/1756-3305-5-6.
• Skotarczak B. 2009. Methods for parasitic protozoa detection in the environmental samples. Parasite, 16, 183–190.
• Stothard D.R., Schroeder-Diedrich J.M., Awwad M.H., Gast R.J., Ledee D.R., Rodriguez-Zaragosa S., Dean C.L., Fuerst P.A., Byers T.J. 1998. The evolutionary history of the genus Acanthamoeba and the identification of eight new 18S rRNA gene sequence types. Journal of Eukaryotic Microbiology, 45, 45–54.
• Stratford M.P., Griffiths A.J. 1978. Variations in the properties and morphology of cysts of Acanthamoeba castellanii. Journal of General Microbiology, 108, 33. DOI: 10.1099/00221287-108-1-33.
• Tanveer T., Hameed A., Muazzam A.G., Jung S.J., Gul A., Matin A. 2013. Isolation and molecular characterization of potentially pathogenic Acanthamoeba genotypes from diverse water resources including household drinking water from Khyber Pakhtunkhwa, Pakistan. Parasitology Research, 112, 2925–2932.
• Thomas V., Herrera-Rimann K., Blanc D.S., Greub G. 2006. Biodiversity of amoebae and amoeba-resisting bacteria in a hospital water network. Applied and Environmental Microbiology, 72(4), 2428–2438. DOI:10.1128/AEM.72.4.2428-2438.2006.
• Tsvetkova N., Schild M., Panaiotov S., Kurdova-Mintcheva R., Gottstein B., Walochnik J., Aspöck H., Lucas M.S., Müller N. 2004. The identification of free-living environmental isolates of amoebae from Bulgaria. Parasitology Research, 92, 405–413. DOI: 10.1007/s00436-003-1052-x.
• Visvesvara G.S., Moura H., Schuster F.L. 2007. Pathogenic and opportunistic free-living amoebae: Acanthamoeba spp., Balamuthia mandrillaris, Naegleria fowleri, and Sappinia diploidea. FEMS Immunology and Medical Microbiology, 50, 1–26. DOI: 10.1111/j.1574-695X.2007.00232.x.
• Yagi S., Schuster F.L., Visvesvara G.S. 2008. Demonstration of Balamuthia and Acanthamoeba mitochondrial DNA in sectioned archival brain and other tissues by the polymerase chain reaction. Parasitology Research, 102, 491–497. DOI: 10.1007/s00436-007-0789-z.