Subthalamic nucleus deep brain stimulation influence on erythrocyte number and hemoglobin levels in a rat model of early Parkinson's disease

• Autorzy: Grembecka B. , Koriat P. , Podlacha M. , Majkutewicz I. , Ptaszek K. , Plucinska K. , Wrona D.
• Języki publikacji: EN

Abstrakty

EN

INTRODUCTION: Subthalamic nucleus deep brain stimulation (STN-DBS) is most effective treatment for Parkinson’s disease (PD) motor symptoms. A number of epidemiological studies have recently highlighted the association between hemoglobin (HGB) levels and PD risk. Interestingly, several lines of evidence confirm that STN-DBS increases regional cerebral blood flow and oxygen concentrations in target brain areas. AIM(S): Considering the close association between oxygen concentration, red blood number (RBC), and HBG, we hypothesized that enhanced blood flow during STN-DBS may influence peripheral RBC parameters in a rat model of early PD. METHOD(S): Male Wistar rats were implanted unilaterally for STN-DBS and received intranigral (substantia nigra pars compacta, SNpc) infusion of 6‑OHDA. After recovery, rats were subjected to STN-DBS for 7 days (1h daily, n=6) or SHAM stimulation (control, n=6). Immediately after collection, peripheral blood samples were analyzed using automated hematology analyzer (Cell Dyn 3700). The RBC number, hematocrit percentage (HCT), HGB concentration, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), and mean corpuscular hemoglobin concentration (MCHC) were measured. PD model was verified by the detection of tyrosine hydroxylase positive neurons in SNpc. For a statistical analysis of the results, SPSS 22.0 software was used. RESULTS: The Student’s t‑test showed that STN‑DBS rats had a significantly higher number of RBC in comparison to the SHAM rats (t(10)=‑2.912; p≤0.05). The HCT percentage slightly increased but differences did not reach statistical significance. Mann‑Whitney U tests showed that HGB level was higher in STN-DBS rats (Z=‑1.290; p≤0.05). CONCLUSIONS: The STN-DBS applied in a rat model of early PD has an influence on RBC number and HGB level. The obtained results suggest that there are peripheral compensation mechanisms for the increased oxygen demand during STN‑DBS in rats. FINANCIAL SUPPORT: Department of Animal and Human Physiology fund.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2019
• Tom: 79
• Numer: Suppl.1
• Opis fizyczny: p.XLVI

Twórcy

autor

Grembecka B.

• Department of Animal and Human Physiology, Faculty of Biology, University of Gdansk, Gdansk, Poland

autor

Koriat P.

• Department of Animal and Human Physiology, Faculty of Biology, University of Gdansk, Gdansk, Poland,

autor

Podlacha M.

• Department of Molecular Biology, Faculty of Biology, University of Gdansk, Gdansk, Poland

autor

Majkutewicz I.

• Department of Animal and Human Physiology, Faculty of Biology, University of Gdansk, Gdansk, Poland

autor

Ptaszek K.

• Department of Animal and Human Physiology, Faculty of Biology, University of Gdansk, Gdansk, Poland

autor

Plucinska K.

• Department of Animal and Human Physiology, Faculty of Biology, University of Gdansk, Gdansk, Poland

autor

Wrona D.

• Department of Animal and Human Physiology, Faculty of Biology, University of Gdansk, Gdansk, Poland