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2011 | 55 | 4 |

Tytuł artykułu

Determination of alkaline phosphatase activity in goat milk and cheese by fluorimetric method as a verification of efficacy of pasteurisation process

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The aim of this study was the verification of completeness of pasteurisation by measuring the alkaline phosphatase activity in goat milk products. Enzyme activity was determined by the use of a fluorimetric method. In total, 75 samples of pasteurised milk, two samples of raw milk, and 17 samples of cheese were examined. Fifty-six percent of pasteurised milk samples had an alkaline phosphatase activity at a level below 50 mU‧L⁻¹, 31% - from 50 to 100 mU‧L⁻¹, and 13% above 100 mU‧L⁻¹. Raw milk had an activity that equalled about 21,500 mU‧L⁻¹. Alkaline phosphatase activity of 82% of the cheese samples was below 2 and 18% - above 2 mU‧L⁻¹. The activity of alkaline phosphatase of all tested samples was below the tentative limits of 350 mU‧L⁻¹ for goat milk and 10 mU‧L⁻¹ for cheese, thus the limits had been supported.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

55

Numer

4

Opis fizyczny

p.705-708,fig.,ref.

Twórcy

autor
  • Department of Hygiene of Food of Animal Origin, National Veterinary Research Institute, 24-100 Pulawy, Poland
autor

Bibliografia

  • 1. Boitelle A. C., Nicolas M.: Alkaline phosphatase activity in goat raw and pasteurised milk. ANSES, EU RL Milk and Milk Products. 11th Workshop of the EU RL for Milk and Milk Products, Vienna, Austria, 2008.
  • 2. Commission Regulation No. 1664/2006 of 6 November 2006 amending Regulation (EC) No. 2074/2005 as regards implementing measures for certain products of animal origin intended for human consumption and repealing certain implementing measures. O J EU 2006, 49, 13-45.
  • 3. Desbourdes C., Nicolas M., Boitelle A.C.: Phosphatase activity in cheese. ANSES, EU RL Milk and Milk Products. 11th Workshop of the EU RL for Milk and Milk Products, Vienna, Austria, 2008.
  • 4. Fox P.F., Kelly A.L.: Indigenous enzymes in milk: overview and historical aspects-part 2. Int Dairy J 2006, 16, 517-532.
  • 5. ISO 11816-1 Milk and milk products. Determination of alkaline phosphatase activity. Part 1: Fluorimetric method for milk and milk-based drinks. International Organization for Standardization, Geneva, Switzerland, 2006.
  • 6. ISO 11816-2 Milk and milk products. Determination of alkaline phosphatase activity. Part 2: Fluorometric method for cheese. International Organization for Standardization, Geneva, Switzerland, 2003.
  • 7. Lorenzen P.C., Martin D., Clawin-Radecker I., Barth K., Knappstein K.: Activities of alkaline phosphatase, γ-glutamyltransferase and lactoperoxidase, in cow, sheep and goat’s milk in relation to heat treatment. Small Rum Res 2010, 89, 18-23.
  • 8. Raynal-Ljutovac K., Par Y.W., Gaucheron F., Bouhallab S.: Heat stability and enzymatic modifications of goat and sheep milk. Small Rum Res 2001, 68, 207-220.
  • 9. Rocco R.M.: Fluorimetric determination of alkaline phosphatase in fluid dairy products: Collaborative study. J Assoc Offic Anal Chem 1990, 73, 842-849.
  • 10. Rosenthal I., Bernstein S., Rosen B.: Alkaline phosphatase activity in Penicillium roqueforti and in blue-veined cheeses. J Dairy Sci 1996, 76, 16-19.
  • 11. Salter R.S., Fitchen J.: Evaluation of chemiluminescence method for measuring alkaline phosphatase activity in whole milk of multiple species and bovine dairy drinks: Interlaboratory study . J AOAC Int 2006, 89, 1061-1070.
  • 12. Wilinska A., Bryjak J., Illeova V., Polakovic M.: Kinetics of thermal inactivation of alkaline phosphatase in bovine and caprine milk and buffer. Int Dairy J 2007, 17, 579-586.
  • 13. Yoshitomi K.: Alkaline phosphatase activity in cheeses measured by fluorometry. Int J Food Sci Tech 2004, 39, 349-353.

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Typ dokumentu

Bibliografia

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