LC determination and stability assessment of macrolide antibiotics azithromycin and spiramycin in bulk and tablet samples

• Autorzy: Mahmoudi A.
• Języki publikacji: EN

Abstrakty

EN

Development and validation of rapid HPLC method for quantifying macrolide antibiotics azithromycin (AZI) and spiramycin (SPI) in bulk and tablet samples is described. Determination was performed on a reversed phase C18 ODB column (250×4.6 nm I.D) at ambient temperature, and employing a UV-detection set at 210 nm. The mobile phase consists of acetonitrile –2-methyl-2- propanol–hydrogenphosphate buffer, pH 6.2, with 1.8% triethylamine (32:8: up to 100, v/v/v), delivered at a flow-rate of 1.1 mL min-1. The assay is linear in concentration ranges of: 0.004–4.8 and 0.0003–1.2 mg mL−1 for azithromycin and spiramycin, respectively, with detection limit of 0.02% for SPI and 0.03% for AZI. Recovery experiments revealed recovery of 98.51–100.82%. The applicability of this method in stability assessment studies is evaluated.

Słowa kluczowe

EN

antibiotic; macrolide; azithromycin; spiramycin; liquid chromatography; high performance liquid chromatography; determination method

• Wydawca: -
• Czasopismo: International Letters of Natural Sciences
• Rocznik: 2015
• Tom: 47
• Opis fizyczny: p.1-10,fig.,ref.

Twórcy

autor

Mahmoudi A.

• Laboratory of Research on Bioactive Products and Biomass Valorization (LRPBVB), Ecole Normale Supérieure–Kouba, P.O. Box 92, Kouba, 16050, Algiers, Algeria

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Identyfikatory

DOI

10.18052/www.scipress.com/ILNS.47.1