In vitro regeneration induced in leaf explants of Citrus limon L. Burm cv. ‘Primofiore’

• Autorzy: Kasprzyk-Pawelec A. , Pietrusiewicz J. , Szczuka E.

Warianty tytułu

PL

Regeneracja in vitro Citrus limon L. Burm cv. ‘Primofiore’ z eksplantatów liściowych

• Języki publikacji: EN

Abstrakty

EN

In vitro organogenesis was studied using Citrus limon L. Burm cv. ‘Primofiore’ leaf explants. The purpose of the present study was to optimize conditions for callogenesis and organogenesis of C. limon. Explants of C. limon were cultured on 16 different media supplemented with various combinations of plant growth regulators, both auxins and cytokinins, such as 6-benzylaminopurine (BAP), naphthalene acetic acid (NAA), 2,4-dichlorophenoxyacetic acid (2,4-D), and kinetin. The best shoot induction was obtained when the leaf explants were cultured on Murashige & Tucker media supplemented with 3.5 mg·L-1 BAP. Histological investigation revealed most likely the initial phase of development of leaf explants during in vitro regeneration of C. limon. t

PL

Organogenezę in vitro badano u Citrus limon L. Burm cv. ‘Primofiore’, wykorzystując eksplantaty z liści. Celem badań była optymalizacja warunków do indukcji tkanki kalusowej oraz regeneracji roślin C. limon. Eksplantaty C. limon wykładano na 16 pożywek różnego rodzaju, które dodatkowo uzupełniano kombinacjami roślinnych regulatorów wzrostu, zarówno auksyn, jak i cytokinin, takimi jak 6-benzylaminopuryna (BAP), kwas naftalenooctowy (NAA), kwas 2,4-dichlorofenoksyoctowy (2,4-D) i kinetyna. Regenerację pędową uzyskano, gdy eksplantaty liściowe wykładano na pożywkę Murashige and Tuckera uzupełnioną 3,5 mg·L-1 BAP. Badania histologiczne pozwoliły na przedstawienie początkowej fazy rozwoju eksplantatów z liści w trakcie regeneracji in vitro C. limon.

Słowa kluczowe

EN

in vitro; plant regeneration; leaf explant; explant; callus induction; auxin; cytokinin; Citrus limon; Primofiore cultivar

• Wydawca: -
• Czasopismo: Acta Scientiarum Polonorum. Hortorum Cultus
• Rocznik: 2015
• Tom: 14
• Numer: 4
• Opis fizyczny: p.143-153,fig.,ref.

Twórcy

autor

Kasprzyk-Pawelec A.

• Department of Plant Anatomy and Cytology, Institute of Biology and Biochemistry, Maria Curie-Sklodowska University in Lublin, Akademicka 19, 20-033 Lublin, Poland

autor

Pietrusiewicz J.

• Department of Plant Anatomy and Cytology, Institute of Biology and Biochemistry, Maria Curie-Sklodowska University in Lublin, Akademicka 19, 20-033 Lublin, Poland

autor

Szczuka E.

• Department of Plant Anatomy and Cytology, Institute of Biology and Biochemistry, Maria Curie-Sklodowska University in Lublin, Akademicka 19, 20-033 Lublin, Poland

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