Hairy root culture of Picrorhiza kurroa Royle ex Benth.: a promising approach for the production of picrotin and picrotoxinin

• Autorzy: Mishra J. , Bhandari H. , Singh M. , Rawat S. , Agnihotri M. , Mishra S. , Purohit S.
• Języki publikacji: EN

Abstrakty

EN

Picrorhiza kurroa Royle ex Benth. is an endangered plant producing various compounds of medicinal importance. Hairy roots of P. kurroa were obtained following cocultivation of shoot tip explants with Agrobacterium rhizogenes strains A 4 and PAT 405. Bacterial strain A 4 appeared to be better than the strain PAT 405 in terms of both growth of respective hairy root cultures and secondary metabolite production. The optimal growth of both the hairy root cultures occurred on halfstrength semisolid medium with 3% sucrose. Picrotin and picrotoxinin from the roots of wild type field grown plants were compared with 8-week-old hairy root cultures induced by the A 4 and PAT 405 strains of A. rhizogenes. Picrotin and picrotoxinin content were evaluated in hairy root cultures as well as roots of field grown plant of P. kurroa. In terms of the production of picrotin and picrotoxinin, the A 4 induced hairy roots appeared to be a better performer than the PAT 405 induced hairy root cultures. The picrotin and picrotoxinin content was highest in 8-week-old A 4 induced hairy roots (8.8 µg/g DW and 47.1 µg/g DW, respectively). Rapid growth of the hairy roots of P. kurroa with in vitro secondary metabolite production potential may offer an attractive alternative to the exploitation of this endangered plant species.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2011
• Tom: 33
• Numer: 5
• Opis fizyczny: p.1941-1846,fig.,ref.

Twórcy

autor

Mishra J.

• GB Pant Institute of Himalayan Environment and Development, Kosi-Katarmal, Almora, Uttarakhand, 263 643, India

autor

Bhandari H.

• GB Pant Institute of Himalayan Environment and Development, Kosi-Katarmal, Almora, Uttarakhand, 263 643, India

autor

Singh M.

• GB Pant Institute of Himalayan Environment and Development, Kosi-Katarmal, Almora, Uttarakhand, 263 643, India

autor

Rawat S.

• GB Pant Institute of Himalayan Environment and Development, Kosi-Katarmal, Almora, Uttarakhand, 263 643, India

autor

Agnihotri M.

• Department of Botany, Dr BR Ambedkar University, Agra, UP, India

autor

Mishra S.

• Department of Biological Science, RDVV, Jabalpur, MP, India

autor

Purohit S.

• GB Pant Institute of Himalayan Environment and Development, Kosi-Katarmal, Almora, Uttarakhand, 263 643, India

Bibliografia

• Choi PS, Kim YD, Choi KP, Chung HJ, Choi DW, Liu JR (2004) Plant regeneration from hairy-root cultures transformed by infection with Agrobacterium rhizogenes in Catharanthus roseus. Plant Cell Rep 22:828–831
• Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15
• Dubey NK, Kumar R, Tripathi P (2004) Global promotion of herbal medicine: India’s opportunity. Curr Sci 86:37–41
• Giri A, Ravindra ST, Dhingra V, Narsu ML (2001) Influence of different strains of Agrobacterium rhizogenes on induction of hairy roots and artemisin production in Artimisia annua. Curr Sci 81:378–382
• Guillon S, Tremouillaux-Guiller J, Pati PK, Rideau M, Gantet P (2006a) Harnessing the potential of hairy roots: dawn of a new era. Trends Biotechnol 24:403–409
• Guillon S, Tremouillaux-Guiller J, Pati PK, Rideau M, Gantet P (2006b) Hairy root research: recent scenario and exciting prospects. Curr Opin Plant Biol 9:341–346
• Hooykass PJJ, Klapwijk PM, Nuti PM, Shilperoot RA, Rorsch A (1977) Transfer of the Agrobacterium tumefaciens Ti-plasmid to a virulent Agrobacterium and Rhizobium explanta. J Gen Microbiol 98:477–487
• Hu Z, Du M (2006) Hairy roots and its application in plant genetic engineering. J Integr Plant Biol 48:121–127
• Hussain A (1984) Conservation of genetic resources of medicinal plants in India. In: Jain SK (ed) Conservation of tropical plant resources. Botanical Survey of India, Howrah, pp 110–117
• Karmarkar SH, Keshavachandran R (2001) Genetic transformation and hairy root induction in Holostmma adakodien K. Schum—a vulnerable medicinal plant. Indian J Exp Biol 39:1263–1267
• Kirtikar KR, Basu BD (1984) Indian medicinal plants, vol Vol III. Bishen Singh Mahendra Pal Singh, Dehradun, pp 1824–1826
• Kittipongpatana N, Hock RS, Porter JR (1998) Production of solasodine by hairy root, callus, and cell suspension cultures of Solanum aviculare Forst. Plant Cell Tiss Org Cult 52:133–143
• Kumar S, Singh J, Shah NC, Ranjan V (1997) Picrorhiza kurroa. In: Sharma A, Kumar A, Tewari R, Hasan SA (eds) Indian Medicinal and aromatic plants facing genetic erosion. CIMAP, Lucknow, pp 169–172
• Pavlov A, Georgiev V, Ilieva M (2005) Betalain biosynthesis by red beet (Beta vulgaris L.) hairy root culture. Process Biochem 40:1531–1533
• Purohit H, Nautiyal BP, Nautiyal MC (2008) Interpopulation variation in Picrorhiza kurroa Royle ex Benth-Step towards identifying genetic variability and elite strains for crop improvement study. Am J Plant Physiol 3(4):154–164
• Ramchandra Rao S, Ravishankar GA (2002) Plant cell cultures: chemical factories of secondary metabolites. Biotech Adv 20:101–153
• Samant SS, Dhar U, Palni LMS (1998) Medicinal plants of Indian Himalaya: diversity, distribution and potential value. Gyanodaya Prakashan, Nainital, p 163
• Tepfer D (1984) Transformation of several species of higher plants by Agrobacterium rhizogenes: sexual transmission of the transformed genotype and phenotype. Cell 37:959–967
• Thakur RS, Puri HS, Hussain A (1989) Major medicinal plants of India. CIMAP, Lucknow, pp 404–407
• Verma PC, Rahman L, Negi AS, Jain DC, Khanuja SPS, Banerjee S (2007) Agrobactrium rhizogenes mediated transformation of Picrorhiza kurroa Royle ex Benth.: establishment and selection of superior hairy root clone. Plant Biotechnol Rep 1:169–174

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