Biochemical characterization of the Arabidopsis KS-type dehydrin protein, whose gene expression is constitutively abundant rather than stress dependent

• Autorzy: Hara M. , Shinoda Y. , Kubo M. , Kashima D. , Takahashi I. , Kato T. , Horiike T.
• Języki publikacji: EN

Abstrakty

EN

Dehydrins are known as plant stress-responsive genes. Arabidopsis thaliana has 10 dehydrin genes. Among them, one of the highly expressed genes is a KS-type dehydrin (At1g54410). However, the gene product, which is a histidine-rich dehydrin whose molecular mass is 11 kDa (AtHIRD11), has not been studied. Thus, we report the biochemical characterization of the AtHIRD11 protein. Although the AtHIRD11 protein was detected in all organs of Arabidopsis, the bolting stem and the flower showed higher accumulation than the other organs, with the AtHIRD11 protein detected in the cambial zone of the stem vasculature. Most of the AtHIRD11 protein was found to be a bound form. The bound AtHIRD11 was solubilized by 1 M NaCl solution. The extracted AtHIRD11 was retained in immobilized metal-affinity chromatography, and eluted by an imidazole gradient. The native AtHIRD11 prepared from Arabidopsis was partially phosphorylated, but further phosphorylated by casein kinase 2 in vitro. Metal-binding assays indicated that Zn²⁺ may be the best metal for AtHIRD11 binding. These results suggest that AtHIRD11 is a metal-binding dehydrin that shows a house-keeping expression in Arabidopsis.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2011
• Tom: 33
• Numer: 6

Twórcy

autor

Hara M.

• Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizouka 422-8529, Japan

autor

Shinoda Y.

• Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizouka 422-8529, Japan

autor

Kubo M.

• Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizouka 422-8529, Japan

autor

Kashima D.

• Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizouka 422-8529, Japan

autor

Takahashi I.

• Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizouka 422-8529, Japan

autor

Kato T.

• Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizouka 422-8529, Japan

autor

Horiike T.

• Division of Global Research Leaders, Shizuoka Unversity, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan

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