Impact of probiotics on numbers, ability to hemolysis and on changes in drug-resistance of selected Escherichia coli isolates recovered from faeces of dairy cattle

• Autorzy: Selwet M. , Galbas M. , Porzucek F.

Warianty tytułu

PL

Wpływ probiotyków na liczebność, zdolność do hemolizy oraz zmiany lekooporności wybranych izolatów Escherichia coli pozyskanych z kału krów mlecznych

• Języki publikacji: EN

Abstrakty

EN

The investigations were conducted on 60 Holstein-Friesian dairy cows (at age 3 year and weight 590 kg) kept in tie-stall barn. The animals were divided into 3 groups of 20 heads each. The control group (K) was fed diets without probiotics, group (EM) – was fed diet with the addition of EM probiotic (dose of 150 ml × t–1 TMR) and group (T) – was fed diet with the addition of ToyoCerin probiotic (dose of 0.2 kg × t–1 TMR). Strains of Escherichia coli were isolated from faeces with the aim to determine their numbers, capability for hemolysis and assessment of their drug-resistance. The isolates were identified as E. coli on the basis of their biochemical properties API 20E (BioMérieux) and the PCR method. When analysing the capability of Escherichia coli for hemolysis, the highest number of haemolytic strains was determined in the faeces of animals fed diets with the addition of the EM probiotic. The examined isolates were characterised by different degrees of resistance to the antibiotics used in experiments. The smallest (P<0.05) number of resistant isolates was determined in group T.

PL

Badaniami objęto 60 krów mlecznych rasy holsztyńsko-fryzyjskiej (w wieku trzech lat, o masie 590 kg) hodowanych w oborze uwięziowej. Zwierzęta podzielono na trzy grupy doświadczalne po 20 osobników. Grupa kontrolna (K) – żywiona bez dodatku probiotyku; grupa (EM) – żywiona z dodatkiem probiotyku EM (dawka 150 ml × t–1 TMR); grupa (T)– żywiona z dodatkiem probiotyku ToyoCerin (dawka 0,2 kg × t–1 TMR). Szczepy Escherichia coli izolowano z kału w celu określenia ich liczebności, zdolności do hemolizy i określenia ich oporności na wybrane antybiotyki. Wyrosłe kolonie Escherichia coli testowano w celu określenia przynależności gatunkowej z zastosowaniem API 20E (BioMérieux) oraz metodą PCR. Badając zdolność Escherichia coli do hemolizy, największą ilość szczepów hemolitycznych oznaczono w kale zwierząt żywionych z dodatkiem probiotyku EM. Badane izolaty wykazywały zróżnicowanie pod względem oporności na użyte w badaniu antybiotyki. Istotnie najmniejszą (P<0,05) liczbę izolatów opornych oznaczono w grupie T.

• Wydawca: -
• Czasopismo: Acta Scientiarum Polonorum. Zootechnica
• Rocznik: 2012
• Tom: 11
• Numer: 2
• Opis fizyczny: p.43-50,fig.,ref.

Twórcy

autor

Selwet M.

• Department of General and Environmental Microbiology, Poznan University of Life Sciences, Szydlowska 50, 60-656 Poznan, Poland

autor

Galbas M.

• Poznan University of Life Sciences, Poznan, Poland

autor

Porzucek F.

• Poznan University of Life Sciences, Poznan, Poland

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Uwagi

PL

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