Phenotypic and molecular characterization of the causal agent of chafer beetle mortality in the wheat fields of the Kurdistan province, Iran

• Autorzy: Karimi K. , Arzanlou M. , Ahari A.B. , Ghazi M.M.
• Języki publikacji: EN

Abstrakty

EN

We report the first case of chafer beetle [Anisoplia austriaca (Herbst 1783)] mortality caused by Actinomucor elegans var. elegans in wheat fields of the Kurdistan province, Iran. For three years, dead larvae of Anisoplia austriaca were collected from wheat fields of the Kurdistan province. Similar isolates of a fast-growing fungus were recovered from all samples. The fungal isolates were identified as A. elegans var. elegans based on morphological and cultural characteristics. The identity of the species was further confirmed using sequence data of the ITS (Internal Transcribed Spacer) region of ribosomal DNA. Koch’s postulates were fulfilled by the inoculation of the larvae of A. austriaca and Galleria mellonella (Linnaeus, 1758) (as the model insect) using the spore suspension of A. elegans var. elegans. The viability of sporangiospores was evaluated using a spore dilution technique on germination medium. The results on the pathogenicity (100% mortality in A. austriaca larvae) and viability tests (germination: 95.45%) demonstrated that A. elegans var. elegans can be considered as a potential biocontrol agent against the chafer beetle. Field experiments are still required to evaluate the capacity of A. elegans as a biological control agent.

Słowa kluczowe

EN

phenotypic characteristics; molecular characteristics; causal agent; chafer beetle zob.scarab beetle; Anisoplia austriaca; scarab beetle; mortality; wheat field; Kurdistan region; Iran

• Wydawca: -
• Czasopismo: Journal of Plant Protection Research
• Rocznik: 2015
• Tom: 55
• Numer: 3
• Opis fizyczny: p.227-234,fig.,ref.

Twórcy

autor

Karimi K.

• Plant Protection Department, Agricultural Faculty, University of Tabriz, P.O. Box 5166614766, Tabriz, Iran

autor

Arzanlou M.

• Plant Protection Department, Agricultural Faculty, University of Tabriz, P.O. Box 5166614766, Tabriz, Iran

autor

Ahari A.B.

• Plant Protection Department, Agricultural Faculty, University of Tabriz, P.O. Box 5166614766, Tabriz, Iran

autor

Ghazi M.M.

• Plant Protection Department, Agricultural and Natural Resources Research Center of Kurdistan, P.O. Box 714, Sanandaj, Iran

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Identyfikatory

DOI

10.1515/jppr-2015-0031