Analyses of plasmids harbouring quinolone resistance determinants in Enterobacteriaceae members

• Autorzy: Erac B. , Yilmaz F.F. , Ozturk I. , Aydemir S.S. , Hosgor-Limoncu M.
• Języki publikacji: EN

Abstrakty

EN

The aim of this study was to explore the plasmid characteristics of eight clinical Enterobacteriaceae strains containing extended broad spectrum beta-lactamases and plasmid-mediated quinolone resistance. Plasmids were transferred by conjugation or transformation and resistance determinants were investigated by PCR. We showed that at least one plasmid harbouring qnrB or qnrS determinant was transferred by conjugation in five isolates. QepA determinant was confirmed to be on a non-conjugative plasmid. We found at least one beta-lactamase gene in seven of the eight clinical isolates having plasmid-mediated quinolone resistance, which indicated that these two resistance determinants were mostly on the same conjugative plasmids.

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2017
• Tom: 66
• Numer: 4
• Opis fizyczny: p.529-532,fig.,ref.

Twórcy

autor

Erac B.

• Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Ege University, Izmir, Turkey

autor

Yilmaz F.F.

• Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Ege University, Izmir, Turkey

autor

Ozturk I.

• Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Ege University, Izmir, Turkey

autor

Aydemir S.S.

• Department of Medical Microbiology, Faculty of Medicine, Ege University, Izmir, Turkey

autor

Hosgor-Limoncu M.

• Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Ege University, Izmir, Turkey

Bibliografia

• Cai X., C. Li, J. Huang and Y. Li. 2011. Prevalence of plasmid mediated quinolone resistance qnr gene in Central China. Afr. J. Microbiol. Res. 5: 975–978.
• CLSI. 2013. Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Third Informational Supplement. CLSI M100-S23. Wayne, PA: Clinical and Laboratory Standards Institute.
• Gay K., A. Robicsek, J. Strahilevitz, C.H. Park, G. Jacoby,T.J. Barrett, F. Medalla, T.M. Chiller and D.C. Hooper. 2006. Plasmid-mediated quinolone resistance in non-Typhi serotypes of Salmonella enterica. Clin. Infect. Dis. 43: 297–304.
• Hoşgör-Limoncu M., B. Eraç, A.N. Yurtman and Ş. Aydemir. 2012. Plasmid-mediated quinolone resistance mechanisms in ESBL positive Escherichia coli and Klebsiella pneumoniae strains at a Tertiary-Care Hospital in Turkey. J. Chemother. 24: 144–149.
• Jacoby G.A. and P. Han. 1996. Detection of extended-spectrum beta-lactamases in clinical solates of Klebsiella pneumoniae and Escherichia coli. J. Clin. Microbiol. 34: 908–911.
• Jacoby G.A., J. Strahilevitz and D.C. Hooper. 2014. Plasmid-mediated quinolone resistance. Microbiol. Spectr. 2: 1–42.
• Kanamori H., R.B. Navarro, H. Yano, L.T. Sombrero, M.R. Caped-ing, S.P. Lupisan, R.M. Olveda, K. Arai, H. Kunishima, Y. Hirakata and M. Kaku. 2011. Molecular characteristics of extended-spectrum β-lactamases in clinical isolates of Enterobacteriaceae from thePhilippines. Acta. Trop. 120: 140–145.
• Kim E.S., J.Y. Jeonga, S.H. Choia, S.O. Lee, S.H. Kim, M.N. Kim, J.H. Woo and Y.S. Kim. 2009. Plasmid-mediated fluoroquinolone efflux pump gene, qepA, in Escherichia coli clinical isolates in Korea. Diagn. Microbiol. Infect. Dis. 65: 335–338.
• Minarini L.A.R., L. Poirel, V. Cattoir, A.L.C. Darini and P. Nordmann. 2008. Plasmid-mediated quinolone resistance determinants among enterobacterial isolates from outpatients in Brazil. J. Antimicrob. Chemother. 62: 474–478.
• Nazik H., B. Bektöre, B. Öngen, M. İlktaç, M. Özyurt, N. Kuvat, O. Baylan, H. Keküllüoğlu, T. Haznedaroğlu and F.M. Keleşoğlu. 2011. Plasmid-mediated quinolone resistance genes in Escherichia coli urinary isolates from two teaching hospitals in Turkey: coexistence of TEM, SHV, CTX-M and VEB-1 type β-lactamases. Trop.J. Pharm. Res. 10: 325–336.
• Pasom W., A. Chanawong, A. Lulitanond, C. Wilailuckana, S. Kenprom and P. Puang-Ngern. 2013. Plasmid-mediated quinolone resistance genes, aac(6¹)-ıb-cr, qnrS, qnrB, and qnrA, in urinary isolates of Escherichia coli and Klebsiella pneumoniae at a teaching hospital, Thailand. Jpn. J. Infect. Dis. 66: 428–432.
• Pfeifer Y., A. Cullik and W. Witte. 2010. Resistance to cephalosporins and carbapenems in Gram-negative bacterial pathogens. Int.J. Med. Microbiol. 300: 371–379.
• Piekarska K., T. Wołkowicz, K. Zacharczuk, M. Rzeczkowska,A. Chróst, E. Bareja, M. Olak and R. Gierczyński. 2015. Co-existence of plasmid-mediated quinolone resistance determinantsand mutations in gyrA and parC among fluoroquinolone-resistant clinical Enterobacteriaceae isolated in a tertiary hospital in Warsaw, Poland. Int. J. Antimicrob. Agents. 45: 238–223.
• Rincón G., M. Radice, M. Giovanakis, J.A. Di Conza andG. Gutkin. 2014. First report of plasmid-mediated fluoroquinolone efflux pump QepA in Escherichia coli clinical isolate ST68, in South America. Diagn. Microbiol. Infect. Dis. 79: 70–72.
• Sambrook J., E.F. Fritsch and T. Maniatis. 1989. Molecular Cloning; A Laboratory Manual, 2nd ed. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
• Taşlı H. and I.H. Bahar. 2005. Molecular characterization of TEM- and SHV-derived extended-spectrum beta-lactamases in hospital-based Enterobacteriaceae in Turkey. Jpn. J. Infect. Dis. 58: 162–167.
• Taşlı H. and I.H. Bahar. 2010. Determination of SHV-2, SHV-5, SHV-12 and CTX-M-3 extended-spectrum beta-lactamases in clinical Enterobacteriaceae isolates. Turk. Klin. J. Med. Sci. 30: 1701–1706.
• Yamane K., J. Wachino, S. Suzuki and Y. Arakawa. (2008). Plasmid-mediated qepA gene among Escherichia coli clinical isolates from Japan. Antimicrob. Agents Chemother. 52: 1564–1566.
• Zhao L., J. Zhang, B. Zheng, Z. Wei, P. Shen, S. Li, L. Li andY. Xiao. 2015. Molecular epidemiology and genetic diversity of fluoroquinolone-resistant Escherichia coli isolates from patients with community-onset infections in 30 Chinese county hospitals. J. Clin. Microbiol. 53: 766–770.

Identyfikatory

DOI

10.5604/01.3001.0010.7084