Zastosowanie metody ELISA w badaniach przeglądowych w kierunku pomoru klasycznego świń

• Autorzy: Pejsak Z. , Kolacz J.

Warianty tytułu

EN

Application of ELISA for detection of antibody against swine fever virus in screening studies

• Języki publikacji: PL

Abstrakty

EN

17 545 field swine sera, from 48 provinces of Poland were tested for the possible presence of antibodies for CSFV by blocking ELISA. The amount of antibodies in the tested serum was expressed as the mean percentage inhibition of the positive signal OD for 2 duplicate wells. For diagnosis, the tested sera were diluted 1:2 and considered positively if they have OD amounting mean value + 25% of positive reference serum. Sera with OD amounting 25%—50% of the positive signal were tested again, using 1:25 as the initial dilution, with CSFV by microplate neutralization test read by NPLA assay and with BVDV by seroneutralization test. Comparison of sera titres with mentioned antigens indicated the presence of BVDV — neutralizing antibodies, while no antibodies caused by CSFV were found. All the sera were found to be free from antibodies to CSV-virus. BVD-virus antibodies were demonstrated in 0.40% of the tested sera.

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 1991
• Tom: 47
• Numer: 09
• Opis fizyczny: s.400-401,tab.,bibliogr.

Twórcy

autor

Pejsak Z.

• Zakład Badania Chorób Świń, Instytut Weterynarii, Al.Partyzantów 57, 24-100 Puławy

autor

Kolacz J.

• Departament Weterynarii, Ministerstwo Rolnictwa i Gospodarki Żywnościowej, ul.Wspólna 30, 00-930 Warszawa

Bibliografia

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• 3. Leforban Y., Have P., Jestin A., Vannier P.: Recl. Med. Vet. 163, 667, 1987.
• 4. Liess В.: Classical swine fever and related viral infections. Msrtinus Nijhoff Publishing. Boston/Dordrecht/Lancaster 1987.
• 5. Pejsak Z., Markowska I., Wójcik J.: Medycyna Wet. 45, 405, 1989.
• 6. Terpstra C.: Vet. Q. 9, 505, 1987.