Immunolocalisation of α and β oestrogen receptors in basolateral amygdala of rabbit males

• Autorzy: Lonc G.
• Języki publikacji: EN

Abstrakty

EN

Expression of a (ER-α) and ß (ER-ß) oestrogen receptors in neurons of basolateral complex of the amygdala was studied in rabbit males. The examinations were carried out on brains of 12 sexualh mature rabbit males weighing 3-4 kg. An immunohistochemical method (IHC) with priman Er-α antibodies, NCL-L6F11 and Er-ß: NCL-ERbeta clone EMR02 was applied. The percentage of immunoreactive neurons was determined. Morphometric and statistical analysis of immunoreactive (ER+) neurons was carried out including the localisation of IHC reaction within the examined neurons. The main place of IHC-ER-α reaction was the cytoplasm of nervous cells, whereas in the examination of IHC-ER-ß, the reaction was observed mainly within cell nucleus. The nuclei of basolateral complex are a region of relatively high percentage of ER-ß+ neurons and lower percentage of ER-α+ neurons in comparison with other nuclei of the amygdala. Basolateral nucleus was characterised by a higher percentage of ER- ß+ neurons in which the reaction was observed in the cytoplasm.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2012
• Tom: 56
• Numer: 1
• Opis fizyczny: p.83-87,fig.,ref.

Twórcy

autor

Lonc G.

• Department of Animal Anatomy and Histology, Faculty of Veterinary Medicine, University of Life Sciences, 20-950 Lublin, Poland

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