High stilbenes accumulation in root cultures of Cayratia trifolia (L.) Domin grown in shake flasks

• Autorzy: Arora J. , Roat C. , Goyal S. , Ramawat K. G.
• Języki publikacji: EN

Abstrakty

EN

The Root cultures of Cayratia trifolia (Vitaceae) a tropical lianas, were maintained in liquid Murashige and Skoog’s medium containing 0.5 mg l⁻¹ NAA, 0.1 mg l⁻¹ kinetin with 3% sucrose. These root cultures when grown with 6% sucrose accumulated stilbenes (piceid, resveratrol, viniferin, ampelopsin) in high amounts, which on elicitation by 500 mg l⁻¹ yeast extract, 50 µM salicylic acid (SA), 50 µM methyl jasmonate (MeJa), 500 µM ethrel added at 25th day, increased up to ninefolds (7.1 mg l⁻¹). Addition of alar or phenylalanine along with the elicitors further enhanced the stilbenes content. In the present study, stilbenes accumulation up to 12 folds (9.2 mg l⁻¹) was obtained with SA and alar. The SA was the most effective in increasing the stilbenes contents while less than control values were recorded in the cells treated with MeJa. The roots could be grown up to 2 l flasks. The present work demonstrates that presence of precursor and sucrose during elicitation at an appropriate time combined with growth retardation significantly increased the production of stilbenes in C. trifolia cell cultures.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2009
• Tom: 31
• Numer: 6
• Opis fizyczny: p.1307-1312,fig.,ref.

Twórcy

autor

Arora J.

• Laboratory of Bio-Molecular Technology, Department of Botany, M.L. Sukhadia University, Udaipur 313001, India

autor

Roat C.

• Laboratory of Bio-Molecular Technology, Department of Botany, M.L. Sukhadia University, Udaipur 313001, India

autor

Goyal S.

• Laboratory of Bio-Molecular Technology, Department of Botany, M.L. Sukhadia University, Udaipur 313001, India

autor

Ramawat K. G.

• Laboratory of Bio-Molecular Technology, Department of Botany, M.L. Sukhadia University, Udaipur 313001, India

Bibliografia

• Basra AS (1994) Mechanisms of plant growth and improved productivity, modern approaches. Marcel Dekker, New York
• Baur J, Sinclair DA (2006) Therapeutic potential of resveratrol: the in vivo evidence. Nat Rev Drug Discov 5:493–506
• Delmas D, Lancon A, Colin D, Jannin B, Latrufe N (2006) Resveratrol as a chemopreventive agent: a promising molecule for fighting cancer. Curr Drug Targets 7:423–442. doi:10.2174/138945006776359331
• El-Sheibany OM, El-Malki NA, Barras-Ali A (2007) Effect of application of growth retardant ALAR on some foliage characters of local cultivar of Chrysanthemum. J Sci Appl 1:15–20
• Goyal S, Ramawat KG (2007) Improved isoflavonoids production by alteration in chemical factors in cell suspension cultures of P. tuberosa. Indian J Exp Biol 45:1068–1072
• Goyal S, Ramawat KG (2008) Increased isoflavonoids accumulation in cell suspension culture of Pueraria tuberosa by elicitors. Indian J Biotechnol 7:378–382
• Krisa S, Larronde F, Budzinski H, Decendit A, Deffieux G, Merillon JM (1999) Stilbenes production by Vitis vinifera cell suspension cultures: methyl jasmonate induction and ¹³C biolabeling. J Nat Prod 62:1688–1690. doi:10.1021/np990239x
• Martin KP, Zhang CL, Hembrom ME, Hembrom ME, Slater A, Madassery J (2008) Adventitious root induction in Ophiorrhiza prostrata: a tool for the production of camptothecin (an anticancer drug) and rapid propagation. Plant Biotechnol Rep 2:163–169. doi:10.1007/s11816-008-0057-4
• Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497. doi:10.1111/j.1399-3054.1962.tb08052.x
• Nassiri-Asl M, Hosseinzadeh H (2009) Review of the pharmacological effects of Vitis vinifera (Grape) and its bioactive compounds. Phytother Res doi:wiley.com/10.1002/ptr.2761
• Paek KY, Chakraborty D, Hahn EJ (2005) Application of bioreactors for large scale production of horticultural and medicinal plants. Plant Cell Tissue Organ Cult 81:287–300. doi:10.1007/s11240-004-6648-z
• Putalun W, Luealon W, De-Eknamkul W, Tanaka H, Shoyama Y (2007) Improvement of artemisinin production by chitosan in hairy root cultures of Artemisia annua L. Biotechnol Lett 29:1143–1146. doi:10.1007/s10529-007-9368-8
• Ramawat KG, Goyal S (2009) Natural products in cancer chemoprevention and chemotherapy. In: Ramawat KG (ed) Herbal drugs: ethnomedicine to modern medicine. Springer, Heidelberg, pp 153–172
• Ramawat KG, Dass S, Mathur M (2009) The chemical diversity of bioactive molecules and therapeutic potential of medicinal plants. In: Ramawat KG (ed) Herbal drugs: ethnomedicine to modern medicine. Springer, Berlin, pp 7–32
• Renaud S, Guégen R, Schenker J et al (1998) Alcohol and mortality in middle-aged men from eastern France. Epidemiology 9:184–188. doi:10.1097/00001648-199803000-00014
• Roat C, Ramawat KG (2009) Morphactin and 2iP markedly enhance accumulation of stilbenes in cell cultures of Cayratia trifolia (L.) Domin. Acta Physiol Plant 31:411–414. doi:10.1007/s11738-008-0233-8
• Roat C, Kumar N, Ramawat KG (2008) Occurrence of stilbenes in unorganized cultures of Cayratia trifolia (L.) Domin. J Herb Med Toxicol 2:11–15
• Savitha BC, Timmaraju R, Bhagyalaksami N, Ravishankar GA (2006) Different biotic and abiotic elicitors influence betalain production in hairy root cultures of Beta vulgaris in shake flask and bioreactor. Process Biochem 41:50–60. doi:10.1016/j.procbio.2005.03.071
• Sudha G, Ravishankar GA (2002) Involvement and interaction of various signaling compounds on the plant metabolic events during defense response, resistance to stress factors, formation of secondary metabolites and their molecular aspects. Plant Cell Tissue Organ Cult 71:181–212. doi:10.1023/A:1020336626361
• Suri SS, Ramawat KG (1995) In vitro hormonal regulation of laticifers differentiation in Calotropis procera. Ann Bot (Lond) 75:477–480. doi:10.1006/anbo.1995.1048
• Verma PC, ur Rahman L, Negi AS, Jain DC, Khunuja SPS, Banerjee S (2007) Agrobacterium rhizogenes-mediated transformation of Picrorhiza kurroa Royle ex Benth: establishment and selection of superior hairy root clone. Plant Biotechnol Rep 1:169–174. doi:10.1007/s11816-007-0029-0
• Waffo-Teguo P, Krisa S, Richard T, Merillon JM (2008) Grapevine stilbenes and their biological effects. In: Ramawat KG, Merillon JM (eds) Bioactive molecules and medicinal plants. Springer, Heidelberg, pp 24–55

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