In vitro fertilizing potential of urethral and epididymal spermatozoa collected from domestic cats (Felis catus)

• Autorzy: Prochowska S. , Nizanski W.
• Języki publikacji: EN

Abstrakty

EN

The aim of this study was to provide a comparative analysis of in vitro fertilizing potential of frozen-thawed urethral and epididymal feline spermatozoa. Both types of semen were collected from 7 cats and cryopreserved in liquid nitrogen. To perform in vitro fertilization, both urethral and epididymal samples from the same individual were thawed and spermatozoa were co-incubated with in vitro matured cat oocytes. Obtained embryos were cultured in vitro for 7 days in a commercial medium. Cleavage rate, morula rate and blastocyst rate were calculated. Experiment was run in 10 replicates. The examined parameters showed no significant differences between urethral and epididymal spermatozoa (p>0.05). Cleavage rate and embryo’s development were highly variable between replicates, even for the different sperm samples collected from one individual. There was no significant correlation between fertilizing capacity of two types of spermatozoa collected from the same male. In this study we confirmed that cryopreserved urethral spermatozoa have equally good fertilizing potential as epididymal ones, and both can be successfully used for in vitro fertilization in cats with the use of commercial medium.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2017
• Tom: 20
• Numer: 1
• Opis fizyczny: p.19-24,fig.,ref.

Twórcy

autor

Prochowska S.

• Department of Reproduction and Clinic of Farm Animals, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, pl. Grunwaldzki 49, 50-366 Wroclaw, Poland

autor

Nizanski W.

• Department of Reproduction and Clinic of Farm Animals, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, pl. Grunwaldzki 49, 50-366 Wroclaw, Poland

Bibliografia

• Bogliolo L, Leoni G, Ledda S, Naitana S, Zedda M, Carluccio A, Pau S (2001) Intracytoplasmic sperm injection of in vitro matured oocytes of domestic cats with frozen-thawed epididymal spermatozoa. Theriogenology 56: 955-967.
• Filliers M, Rijsselaere T, Bossaert P, Zambelli D, Anastasi P, Hoogewijs M, Van Soom A (2010) In vitro evaluation of fresh sperm quality in tomcats: a comparison of two collection techniques. Theriogenology 74: 31-39.
• Freistedt P, Stojkovic M, Wolf E (2001) Efficient in vitro production of cat embryos in modified synthetic oviduct fluid medium: effects of season and ovarian status. Biol Reprod 65: 9-13.
• Herrick JR, Bond JB, Magarey GM, Bateman HL, Krisher RL, Dunford SA, Swanson WF (2007) Toward a feline-optimized culture medium: impact of ions, carbohydrates, essential amino acids, vitamins, and serum on development and metabolism of in vitro fertilization-derived feline embryos relative to embryos grown in vivo. Biol Reprod 76: 858-870.
• Lawitts JA, Biggers JD (1991) Overcoming the 2-cell block by modifying standard components in a mouse embryo culture medium. Biol Reprod 45: 245-251.
• Li H, Hung PH, Suarez SS (2015) Ejaculated mouse sperm enter cumulus-oocyte complexes more efficiently in vitro than epididymal sperm. PLoS One 10: e0127753.
• Luvoni GC, Chigioni S, Perego L, Lodde V, Modina S, Luciano AM (2006) Effect of gonadotropins during in vitro maturation of feline oocytes on oocyte-cumulus cells functional coupling and intracellular concentration of glutathione. Anim Reprod Sci 96: 66-78.
• Niżanski W, Dejneka GJ, Klimowicz M, Dubiel A (2005) Evaluating some properties of domestic cat epididymal spermatozoa and their cryopreservation. Med Weter 61: 173-178.
• Pope CE (2000) Embryo technology in conservation efforts for endangered felids. Theriogenology 53: 163-174.
• Prochowska S, Niżański W, Ochota M, Partyka A (2015) Characteristics of urethral and epididymal semen collected from domestic cats – A retrospective study of 214 cases. Theriogenology 84: 1565-1571.
• Prochowska S, Niżański W, Partyka A. (2016) Comparative analysis of in vitro characteristics of fresh and frozen-thawed urethral and epididymal spermatozoa from cats (Felis domesticus). Theriogenology 86: 2063-2072.
• Rath D, Niemann H (1997) In vitro fertilization of porcine oocytes with fresh and frozen-thawed ejaculated or frozen-thawed epididymal semen obtained from identical boars. Theriogenology 47: 785-793.
• Sananmuang T, Tharasanit T, Nguyen C, Phutikanit N, Techakumphu M (2011) Culture medium and embryo density influence on developmental competence and gene expression of cat embryos. Theriogenology 75: 1708-1719.
• Thuwanut P, Arya N, Comizzoli P, Chatdarong K (2015) Effect of extracellular adenosine 5’-triphosphate on cryopreserved epididymal cat sperm intracellular ATP concentration, sperm quality, and in vitro fertilizing ability. Theriogenology 84: 702-709.
• Watson PF (2000) The causes of reduced fertility with cryopreserved semen. Anim Reprod Sci 60-61: 481-492.
• Waurich R, Ringleb J, Braun BC, Jewgenow K (2010) Embryonic gene activation in in vitro produced embryos of the domestic cat (Felis catus). Reproduction 140: 531-540.
• Zambelli D, Merlo B, Iacono E, Prati F, Belluzzi S (2006) Fertilizing ability of electro-ejaculated cryopreserved semen in the domestic cat. Reprod Domest Anim 41: 137-141.
• Zambelli D, Prati F, Cunto M, Iacono E, Merlo B (2008) Quality and in vitro fertilizing ability of cryopreserved cat spermatozoa obtained by urethral catheterization after medetomidine administration. Theriogenology 69: 485-490.
• Zhang BR, Larsson B, Lundeheim N, Rodriguez-Martinez H (1997) Relationship between embryo development in vitro and 56-day nonreturn rates of cows inseminated with frozen-thawed semen from dairy bulls. Theriogenology 48: 221-231.

Identyfikatory

DOI

10.1515/pjvs-2017-0003