Influence of beta2- and beta3-adrenoceptor agonists on contractile activity of the porcine myometrium in the luteal phase and the first days of pregnancy

• Autorzy: Markiewicz W. , Jaroszewski J.J.
• Języki publikacji: EN

Abstrakty

EN

This study analysed the relaxant properties of salbutamol (β₂₋adrenoceptors agonist) and BRL 37344 (β₃₋adrenoceptors agonist) regarding the contractility of porcine myometrium on days 10-14 of the oestrous cycle (cyclic group; n = 10) and on days 3-5 of pregnancy (early pregnant group; n = 6). The activity of myometrial strips (tension, frequency and amplitude) was recorded under isometric conditions using force transducers. The contractility was assessed further following the administration of increasing concentrations of the agonists (10⁻⁹-10⁻⁴ M), both with and without β-adrenoceptor antagonists (butaxamine – a selective β₂₋ adrenoceptor antagonist, propranolol- a non-selective β₁₋and β₂₋adrenoceptor antagonist and bupranolol – a non-selective β₁₋, β₂₋ and β₃₋adrenoceptor antagonist) at a concentration of 10⁻⁴ M. Although neither salbutamol nor BRL 37344 caused changes in the tension, at the highest concentrations they decreased the frequency and amplitude of contractions. These changes were more evident after salbutamol treatment and in the early pregnant group. Antagonists given alone did not cause changes in the parameters examined but changed some activity of the agonists. Butoxamine reduced the decrease in frequency and amplitude induced by salbutamol and produced a decrease in the tension after BRL 37344 treatment in the early pregnant group. Propranolol reduced the decrease in frequency and amplitude induced by salbutamol in both examined groups and did not cause significant changes in BRL 37344 activity. The administration of bupranolol before salbutamol treatment caused an increase in the tension and reduced the decrease in the frequency in the cyclic group. Moreover, bupranolol eliminated a decrease in frequency and induced an increase in amplitude caused by BRL 37344 in both groups and these changes were more evident in the early pregnant group. The data indicates that both β₂₋ and β₃₋adenoreceptors are involved in the regulation of the contractility in both groups, but the changes after agonists and antagonists treatment are more evident in the early pregnant myometrium.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2017
• Tom: 20
• Numer: 1
• Opis fizyczny: p.111-121,fig.,ref.

Twórcy

autor

Markiewicz W.

• Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego Street 13, 10-718 Olsztyn, Poland

autor

Jaroszewski J.J.

• Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego Street 13, 10-718 Olsztyn, Poland

Bibliografia

• Bardou M, Loustalot C, Cortijo J, Simon B, Naline E, Dumas M, Esteve S, Croci T, Chalon P, Frydman R, Sagot P, Manara L, Morcillo EJ, Advenier C (2000) Functional, biochemical and molecular biological evidence for a possible beta(3)-adrenoceptor in human near-term myometrium. Br J Pharmacol 130: 1960-1966.
• Bardou M, Rouget C, Breuiller-Fouche M, Loustalot C, Naline E, Sagot P, Frydman R, Morcillo EJ, Advenier C, Leroy MJ, Morrison JJ (2007) Is the beta3-adrenoceptor (ADRB3) a potential target for uterorelaxant drugs? BMC Pregnancy Childbirth 7 (Suppl 1): S14.
• Cao J, Shayibuzhati M, Tajima T, Kitazawa T, Taneike T (2002) In vitro pharmacological characterization of the prostanoid receptor population in the non-pregnant porcine myometrium. Eur J Pharmacol 442: 115-123.
• Clouse AK, Riedel E, Hieble JP, Westfall TD (2007) The effects and selectivity of beta- drenoceptor agonists in rat myometrium and urinary bladder. Eur J Pharmacol 573: 184-189.
• de Heus R, Mol BW, Erwich JJ, van Geijn HP, Gyselaers WJ, Hanssens M, Harmark L, van Holsbeke CD, Duvekot JJ, Schobben FF, Wolf H, Visser GH (2009) Adverse drug reactions to tocolytic treatment for preterm labour: prospective cohort study. BMJ 338: b744.
• Dennedy MC, Friel AM, Gardeil F, Morrison JJ (2001) Beta-3 versus beta-2 adrenergic agonists and preterm labour: in vitro uterine relaxation effects. BJOG 108: 605-609.
• Dennedy MC, Houlihan DD, McMillan H, Morrison JJ (2002) Beta2- and beta3- adrenoreceptor agonists: human myometrial selectivity and effects on umbilical artery tone. Am J Obstet Gynecol 187: 641-647.
• Engstorm T, Bratholm P, Vilhardt H, Christensen NJ (1997) Effect of pregnancy on rat myometrial beta2-adrenoceptor mRNA and isoproterenol-induced relaxation of isolated uterine strips. J Endocrinol 153: 393-399.
• Fanchin R, Righini C, Olivennes F, Taylor S, de Ziegler D, Frydman R (1998) Uterine contractions at the time of embryo transfer alter pregnancy rates after in-vitro fertilization. Hum Reprod 13: 1968-1974.
• Jana B, Jaroszewski JJ, Kucharski J, Koszykowska M, Górska J, Markiewicz W (2010) Participation of prostaglandin E2 in contractile activity of inflamed porcine uterus. Acta Vet Brno 79: 249-259.
• Jana B, Jaroszewski JJ, Czarzasta J, Włodarczyk M, Markiewicz W (2013) Synthesis of prostacyclin and its effect on the contractile activity of the inflamed porcine uterus. Theriogenology 79: 470-485.
• Kitazawa T, Uchiyama F, Hirose K, Taneike T (1999) Characterization of the muscarinic receptor subtype that mediates the contractile response of acetylcholine in the swine myometrium. Eur J Pharmacol 367: 325-334.
• Kitazawa T, Nakagoshi K, Teraoka H, Taneike T (2001) 5-HT7 receptor and beta2-adrenoceptor share in the inhibition of porcine uterine contractility in a muscle layer-dependent manner. Eur J Pharmacol 433: 187-197.
• Kitazawa T, Hatakeyama H, Cao J, Taneike T (2003) Pregnancy- associated changes in responsiveness of the porcine myometrium to bioactive substances. Eur J Pharmacol 469: 135-144.
• Kucharski J, Jaroszewski JJ, Jana B, Górska J, Kozłowska A, Markiewicz W (2007) The influence of inflammatory process on prostaglandin F2alpha contractile activity in porcine uterus. J Anim Feed Sci 16: 654-667.
• Kurowicka B, Franczak A, Oponowicz A, Kotwica G (2005) In vitro contractile activity of porcine myometrium during luteolysis and early pregnancy: effect of oxytocin and progesterone. Reprod Biol 5: 151-169.
• Larsen JJ (1979) Beta-adrenoceptors in the pregnant and non-pregnant myometrium of the goat and cow. Acta Pharmacol Toxicol (Copenh) 44: 132-138.
• Leiser R, Zimmermann W, Sidler X, Christen A (1988) Normal cyclical morphology of the endometrium and ovary of swine. Tierarztl Prax 16: 261-280.
• Liu YL, Nwosu UC, Rice PJ (1998) Relaxation of isolated human myometrial muscle by beta2-adrenergic receptors but not beta1-adrenergic receptors. Am J Obstet Gynecol 179: 895-898.
• Markiewicz W, Kamińska K, Bogacki M, Maślanka T, Jaroszewski JJ (2012) Participation of analogues of lysophosphatidic acid (LPA): oleoyl-sn-glycero-3-phosphate (L-alpha-LPA) and 1-oleoyl-2-O-methyl-rac-glycerophosphothionate (OMPT) in uterine smooth muscle contractility of the pregnant pigs. Pol J Vet Sci 15: 635-643.
• Markiewicz W, Bogacki M, Blitek M, Jaroszewski JJ (2016) Comparison of the porcine uterine smooth muscle contractility on days 12-14 of the estrous cycle and pregnancy. Acta Vet Scand 58: 20.
• Markiewicz W, Jaroszewski JJ (2016) β₂₋ and β₃₋adrenergic receptors stimulation relaxes porcine myometrium in the peri-implantation period. J Anim Sci 94: 4611-4618.
• Minorics R, Gáspár R, Ghl A, Klukovits A, Falkay G (2009) Progesterone decreases the relaxing effect of the beta3-adrenergic receptor agonist BRL 37344 in the pregnant rat myometrium. Reproduction 138: 383-390.
• Motazedian S, Ghaffarpasand F, Mojtahedi K, Asadi N (2010) Terbutaline versus salbutamol for suppression of preterm labor: a randomized clinical trial. Ann Saudi Med 30: 370-375.
• Parida S, Uttam Singh T, Ravi Prakash V, Mishra SK (2013) Molecular and functional characteristics of β₃₋adrenoceptors in late pregnant mouse uterus: a comparison with β₂₋adrenoceptors. Eur J Pharmacol 700: 74-79.
• Pędzińska-Betiuk A, Modzelewska B, Kostrzewska A, Jóźwik M (2008) Influence of potent and selective-adrenoceptor agonist, CL 316243, on the contractile response of non-pregnant human myometrium. J Physiol Pharmacol 9: 43-54.
• Pędzińska-Betiuk A, Modzelewska B, Jóźwik M, Jóźwik M, Kostrzewska A (2011) Differences in the effects of beta2-and beta3-adrenoceptor agonists on spontaneous contractions of human nonpregnant myometrium. Ginekol Pol 82: 918-924.
• Rouget C, Bardou M, Breuiller-Fouche M, Loustalot C, Qi H, Naline E, Croci T, Cabrol D, Advenier C, Leroy MJ (2005) Beta3-adrenoceptor is the predominant beta-adrenoceptor subtype in human myometrium and its expression is up-regulated in pregnancy. J Clin Endocrinol Metab 90: 1644-1650.
• Taneike T, Narita T, Kitazawa T, Bando S, Teraoka H, Ohga A (1995) Binding and functional characterization of alpha-2 adrenoceptors in isolated swine myometrium. J Auton Pharmacol 15: 93-105.
• Ursino MG, Vasina V, Raschi E, Crema F, De PontiF (2009) The beta3-adrenoceptor as a therapeutic target: current perspectives. Pharmacol Res 59: 221-234.
• Wasielak M, Więsak T, Bogacka I, Jalali BM, Bogacki M (2016) Zygote arrest 1, nucleoplasmin 2 and developmentally associated protein 3 mRNA profiles throughout porcine embryo development in vitro. Theriogenology 86: 2254-2262.
• Yurtcu N, Cetin A, Karadas B, Gonca Imir A, Kaya T, Erselcan T, Bagcivan I, Cetin M (2006) Comparison of effects of formoterol and BRL 37344 on isolated term-pregnant rat myometrial strips in vitro. Eur J Pharmacol 530: 263-269.
• Zięcik AJ, Wacławik A, Kaczmarek MM, Blitek A, Jalali BM, Andronowska A (2011) Mechanisms for the establishment of pregnancy in the pig. Reprod Domest Anim 3: 31-41.

Identyfikatory

DOI

10.1515/pjvs-2017-0015