Empirical assessment of non-invasive population genetics in bats: Comparison of DNA quality from faecal and tissue samples

• Autorzy: Boston E.S.M. , Puechmaille S. J. , Scott D. D. , Buckley D. J. , Lundy M. G. , Montgomery I. W. , Prodohl P. A. , Teeling E. C.
• Języki publikacji: EN

Abstrakty

EN

Non-invasive population genetics has become a valuable tool in ecology and conservation biology, allowing genetic studies of wild populations without the need to catch, handle or even observe the study subjects directly. We address some of the concerns regarding the limitations of using non-invasive samples by comparing the quality of population genetic information gained through DNA extracted from faecal samples and biopsy samples of two elusive bat species, Myotis mystacinus and Myotis nattereri. We demonstrate that DNA extracted from faeces and tissue samples gives comparable results for frequency based population genetic analyses, despite the occurrence of genotyping errors when using faecal DNA. We conclude that non-invasive genetic sampling for population genetic analysis in bats is viable, and although more labour-intensive and expensive, it is an alternative to tissue sampling, which is particularly pertinent when specimens are rare, endangered or difficult to capture.

• Wydawca: -
• Czasopismo: Acta Chiropterologica
• Rocznik: 2012
• Tom: 14
• Numer: 1
• Opis fizyczny: p.45-52

Twórcy

autor

Boston E.S.M.

• Centre for Irish Bat Research, University College Dublin, Science Centre West, Belfield, Dublin 4, Ireland

autor

Puechmaille S. J.

• Centre for Irish Bat Research, University College Dublin, Science Centre West, Belfield, Dublin 4, Ireland
• Max Planck Institute for Ornithology, Sensory Ecology Group, Eberhard-Gwinne-Strasse, 82319 Seewiesen, Germany

autor

Scott D. D.

• Centre for Irish Bat Research, Queen's University Belfast, Medical and Biological Centre, 97 Lisburn Rd., Belfast, Northern Ireland

autor

Buckley D. J.

• Centre for Irish Bat Research, University College Dublin, Science Centre West, Belfield, Dublin 4, Ireland

autor

Lundy M. G.

• Centre for Irish Bat Research, Queen's University Belfast, Medical and Biological Centre, 97 Lisburn Rd., Belfast, Northern Ireland

autor

Montgomery I. W.

• Centre for Irish Bat Research, Queen's University Belfast, Medical and Biological Centre, 97 Lisburn Rd., Belfast, Northern Ireland

autor

Prodohl P. A.

• Centre for Irish Bat Research, Queen's University Belfast, Medical and Biological Centre, 97 Lisburn Rd., Belfast, Northern Ireland

autor

Teeling E. C.

• Centre for Irish Bat Research, University College Dublin, Science Centre West, Belfield, Dublin 4, Ireland

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