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2013 | 69 | 04 |

Tytuł artykułu

False positive serological reactions to brucellosis in pigs: a growing problem in international trade

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The diagnosis of brucellosis in pigs is based almost entirely on serological assays. None of the tests has been shown to be reliable in routine diagnosis in individual pigs. The biggest problem are false positive serological reactions (FPSRs) caused primarily by Yersinia enterocolitica O:9. The OPS component of the sLPS of Brucella is almost identical with that of Y. enterocolitica O:9. Thus no routinely used serological tests based on this antigen can distinguish between antibodies raised to these two infections. This paper presents the results of the examinations of 6 batches of pigs (total of 452 serum samples) traded between countries and causing major diagnostic problems. Positive reactions in RBT, SAT, CFT and I-ELISA were observed in all these batches of animals. Additionally 2 out of 21 samples from one of the batches were positive in 2-Me. FPSRs in the diagnosis of pigs for brucellosis seem to be a growing problem in international trade. The absence of provisions explicitly regulating the problem of FPSRs may have serious consequences, such as the slaughter of animals or even international repercussions. Clear guidelines for dealing with such cases should therefore be formulated.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

69

Numer

04

Opis fizyczny

p.241-244,ref.

Twórcy

autor
  • Department of Microbiology, National Veterinary Research Institute, 24-100 Pulawy, Poland
autor
  • Department of Microbiology, National Veterinary Research Institute, 24-100 Pulawy, Poland
autor
  • Department of Microbiology, National Veterinary Research Institute, 24-100 Pulawy, Poland
autor
  • Department of Microbiology, National Veterinary Research Institute, 24-100 Pulawy, Poland
autor
  • Department of Microbiology, National Veterinary Research Institute, 24-100 Pulawy, Poland
autor
  • Department of Microbiology, National Veterinary Research Institute, 24-100 Pulawy, Poland
autor
  • Department of Microbiology, National Veterinary Research Institute, 24-100 Pulawy, Poland

Bibliografia

  • 1.Council Directive 64/432/EEC of 26 June 1964 on animal health problems affecting intra-Community trade in bovine animals and swine (OJ No. 121, 29.7.1964, p. 1977/64).
  • 2.Council Directive 90/429/EEC of 26 June 1990 laying down the animal health requirements applicable to intra-Community trade in and imports of semen of domestic animals of the porcine species (OJ L 224, 18.8.1990, p. 62-73).
  • 3.Gyuranecz M., Erdélyi K., Makrai L., Fodor L., Szépe B., Ráczné Mészáros A., Dán A., Dencső L., Fassang E., Szeredi L.: Brucellosis of the European Brown Hare (Lepus europaeus). J. Comp. Path. 2011, 145, 1-5.
  • 4.Instruction no 26/2003 of the Chief Veterinary Officer, Warsaw 2003.
  • 5.Instruction no 27/2003 of the Chief Veterinary Officer, Warsaw 2003.
  • 6.Instruction no 28/2003 of the Chief Veterinary Officer, Warsaw 2003.
  • 7.Jungersen G., Sörensen V., Giese S. B., Stack J. A., Riber U.: Differentiation between serological responses to Brucella suis and Yersinia enterocolitica serotype O:9 after natural or experimental infection in pigs. Epidemiol. Infect. 2006, 134, 347-357.
  • 8.Królak M., Stryszak M.: Standard technique of 2-mercaptoethanol test in diagnosis of animal brucellosis. Nat. Vet. Res. Inst. Pulawy, Poland 1979.
  • 9.Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, OIE. Paris, France 2009.
  • 10.Porcine brucellosis (Brucella suis). Scientific opinion of the Panel on Animal Health and Welfare. EFSA Journal 1144, 1-112.
  • 11.Singh D. K., Warayan K. G.: Serological study of swine yersiniosis in a farm. Indian J. Anim. Sci. 1991, 5, 506-508.
  • 12.Szulowski K., Iwaniak W., Pilaszek J.: Porcine brucellosis in Poland: problems accompanying serological surveys conducted in 1995-2000. Bull. Vet. Inst. Pulawy 2001, 45, 153-161.
  • 13.Szulowski K., Iwaniak W., Pilaszek J., Truszczyñski M., Chrobociñska M.: The ELISA for the examination of hare sera for anti-Brucella antibodies. Comp. Immun. Microbiol. Infect. Dis. 1999, 22, 33-40.
  • 14.Szulowski K., Pilaszek J., Iwaniak W.: Application of meat juice in diagnosis of brucellosis in hares and wild boars by ELISA. Bull. Vet. Inst. Pulawy 2000, 44, 45-52.
  • 15.Weiner M., Szulowski K., Iwaniak W.: Porcine brucellosis - evidence of the role of Yersinia enterocolitica O:9 in occurrence of false positive serological reactions. Pol. J. Vet. Sci. in press.
  • 16.Weiner M., Szulowski K., Iwaniak W.: The value of Fluorescence Polarisation Assay in verification of problematic sera from cattle and pigs for brucellosis. Pol. J. Vet. Sci. 2012, 15, 801-802.
  • 17.Weiner M., Złotnicka J., Iwaniak W., Szulowski K.: Development of a multiplex PCR for identification of Brucella sp. and cross-reacting Yersinia enterocolitica O:9. Bull. Vet. Inst. Pulawy 2011, 55, 603-607.
  • 18.Wiśniowski J., Królak M., Drożdżyńska M.: Standard technique of anti-globulin test in diagnosis of bovine brucellosis. Nat. Vet. Res. Inst. Pulawy, Poland 1978.
  • 19.Wrathall A. E., Broughton E. S., Gill K. P., Goldsmith G. P.: Serological reactions to Brucella species in British pigs. Vet. Rec. 1993, 132, 449-454.

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Bibliografia

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