Alteration of nutrient allocation and transporter genes expression in rice under N, P, K, and Mg deficiencies

• Autorzy: Cai J. , Chen L. , Qu H. , Lian J. , Liu W. , Hu Y. , Xu G.
• Języki publikacji: EN

Abstrakty

EN

Nitrogen (N), phosphorus (P), potassium (K), and magnesium (Mg) have essential physiological functions in plants. Their interactions in plants are not fully understood especially at the molecular level. In this study, we detected the physiological and molecular responses of rice plants at the vegetative growth phase to N, P, K, and Mg starvations. Deficiencies of N and P resulted in accumulation of soluble sugar and starch in the leaves. The root to shoot ratio increased under N and P deficiencies, but decreased under K and Mg deficiencies. In addition, deficiency of either K or Mg resulted in accumulation of the other cation in shoots. Moreover, K starvation decreased both K and soluble sugar contents in the roots pronouncedly. RT-PCR analysis showed that several sugar transporter genes in the leaves orchestrated with sugar accumulation induced by the nutrient shortages. Expression of a high affinity K transporter gene (OsHAK1) and a putative Mg transporter gene (OsMGT) showed opposite down- and up-regulation in the roots by K starvation. These findings suggest that deficiencies of the major nutrients suppressed the export of carbohydrates from source leaves. The regulated sugar and nutrient transporter genes investigated in this study could be used for elucidating the molecular mechanism of plants in their adaptation to varied nutrient supply.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2012
• Tom: 34
• Numer: 3
• Opis fizyczny: p.939-946,fig.,ref.

Twórcy

autor

Cai J.

• College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China

autor

Chen L.

• College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China

autor

Qu H.

• College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China

autor

Lian J.

• College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China

autor

Liu W.

• High-tech Research Center, Shandong Academy of Agricultural Sciences, 250100 Jinan, China

autor

Hu Y.

• College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China

autor

Xu G.

• College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China

Bibliografia

• Aoki N, Hirose T, Scofield GN, Whitfeld PR, Furbank RT (2003) The sucrose transporter gene family in rice. Plant Cell Physiol 44:223–232
• Barker L, Kühn C, Weise A, Schulz A, Gebhardt C, Hirner B, Hellmann H, Schulze W, Ward JM, Frommer WB (2000) SUT2, a putative sucrose sensor in sieve elements. Plant Cell 12:1153–1164
• Barth I, Meyer S, Sauer N (2003) PmSUC3: characterization of a SUT2/SUC3-type sucrose transporter from Plantago major. Plant Cell 15:1375–1385
• Berkowitz GA, Wu W (1993) Magnesium, potassium flux and photosynthesis. Magnes Res 6:257–265
• Cakmak I, Hengeler C, Marschner H (1994) Partitioning of shoot and root dry matter and carbohydrates in bean plants suffering from phosphorus, potassium and magnesium deficiency. J Exp Bot 45:1245–1250
• Carpaneto A, Koepsell H, Bamberg E, Hedrich R, Geiger D (2010) Sucrose- and H-dependent charge movements associated with the gating of sucrose transporter ZmSUT1. PLoS One 5:e12605
• Ding Y, Luo W, Xu G (2006) Characterisation of magnesium nutrition and interaction of magnesium and potassium in rice. Ann Appl Biol 149:111–123
• Doman DC, Geiger DR (1979) Effect of exogenously supplied foliar potassium on phloem loading in Beta vulgaris L. Plant Physiol 64:528–533
• Gajdanowicz P, Michard E, Sandmann M, Rocha M, Corrêa LG, Ramírez-Aguilar SJ, Gomez-Porras JL, González W, Thibaud JB, van Dongen JT, Dreyer I (2011) Potassium K⁺ gradients serve as a mobile energy source in plant vascular tissues. Proc Natl Acad Sci USA 108:864–869
• Gibson SI (2005) Control of plant development and gene expression by sugar signaling. Curr Opin Plant Biol 8:93–102
• Hansen J, Møller IB (1975) Percolation of starch and soluble carbohydrates from plant tissue for quantitative determination with anthrone. Anal Biochem 68:87–94
• Hayashi H, Chino M (1990) Chemical composition of phloem sap from the uppermost internode of the rice plant. Plant Cell Physiol 31:247–251
• Hermans C, Hammond JP, White PJ, Verbruggen N (2006) How do plants respond to nutrient shortage by biomass allocation? Trends Plant Sci 12:531–532
• Hirose T, Zhang Z, Miyao A, Hirochika H, Ohsugi R, Terao T (2010) Disruption of a gene for rice sucrose transporter, OsSUT1, impairs pollen function but pollen maturation is unaffected. J Exp Bot 61:3639–3646
• Horie T, Sugawara M, Okada T, Taira K, Kaothien-Nakayama P, Katsuhara M, Shinmyo A, Nakayama H (2011) Rice sodiuminsensitive potassium transporter, OsHAK5, confers increased salt tolerance in tobacco BY2 cells. J Biosci Bioeng 111:346–356
• Karley AJ, White PJ (2009) Moving cationic minerals to edible tissues: potassium, magnesium, calcium. Curr Opin Plant Biol 12:291–298
• Karley AJ, White PJ (2010) Potassium. Plant Cell Monogr 17:199–224
• Kumar A, Silim SN, Okamoto M, Siddiqi MY, Glass AD (2003) Differential expression of three members of the AMT1 gene family encoding putative high-affinity NH₄⁺ transporters in roots of Oryza sativa subspecies indica. Plant Cell Environ 26:907–914
• Lee RB (1982) Selectivity and kinetics of ion uptake by barley plants following nutrient deficiency. Ann Bot 50:429–449
• Lee RB (1993) Control of net uptake of nutrients by regulation of influx in barley plants recovering from nutrient deficiency. Ann Bot 72:223–230
• Li CY, Weiss D, Goldschmidt EE (2003) Effects of carbohydrate starvation on gene expression in citrus root. Planta 217:11–20
• Li L, Tutone AF, Drummond RS, Gardner RC, Luan S (2001) A novel family of magnesium transport genes in Arabidopsis. Plant Cell 13:2761–2775
• Li L, Kim BG, Cheong YH, Pandey GK, Luan S (2006) A Ca²⁺ signalling pathway regulates a K⁺ channel for low-K response in Arabidopsis. Proc Natl Acad Sci USA 103:12625–12630
• Maathuis FJ (2009) Physiological functions of mineral macronutrients. Curr Opin Plant Biol 12:250–258
• Maathuis FJ, Amtmann A (1999) K⁺ nutrition and Na⁺ toxicity: the basis of cellular K⁺/Na⁺ ratios. Ann Bot 84:123–133
• Matsukura C, Saitoh T, Hirose T, Ohsugi R, Perata P, Yamaguchi J (2000) Sugar uptake and transport in rice embryo. Expression of companion cell-specific sucrose transporter (OsSUT1) induced by sugar and light. Plant Physiol 124:85–93
• Miller AJ, Shen Q, Xu G (2009) Freeways in the plant: transporters for N, P and S and their regulation. Curr Opin Plant Biol 12:284–290
• Okada T, Nakayama H, Shinmyo A, Yoshida K (2008) Expression of OsHAK genes encoding potassium ion transporters in rice. Plant Biotechnol 25:241–245
• Qi Z, Hampton CR, Shin R, Barkla BJ, White PJ, Schachtman DP (2008) The high affinity K⁺ transporter AtHAK5 plays a physiological role in planta at very low K⁺ concentrations and provides a caesium uptake pathway in Arabidopsis. J Exp Bot 59:595–607
• Ramsperger-Gleixner M, Geiger D, Hedrich R, Sauer N (2004) Differential expression of sucrose transporter and polyol transporter genes during maturation of common plantain companion cells. Plant Physiol 134:147–160
• Scofield GN, Hirose T, Aoki N, Furbank RT (2007) Involvement of the sucrose transporter, OsSUT1, in the long-distance pathway for assimilate transport in rice. J Exp Bot 58:3155–3169
• Shaul O, Hilgemann DW, de-Almeida-Engler J, Van Montagu M, Inz D, Galili G (1999) Cloning and characterization of a novel Mg²⁺/H⁺ exchanger. EMBO J 18:3973–3980
• Shaul O (2002) Magnesium transport and function in plants: the tip of the iceberg. Biometals 15:309–323
• Stadler R, Brandner J, Schulz A, Gahrtz M, Sauer N (1995) Phloem loading by the PmSUC2 sucrose carrier from Plantago major occurs into companion cells. Plant Cell 7:1545–1554
• Sun Y, Reinders A, La Fleur KR, Mori T, Ward JM (2010) Transport activity of rice sucrose transporters OsSUT1 and OsSUT5. Plant Cell Physiol 51:114–122
• Tuncay Ö , Irget ME, Gü l A, Budak N (1999) Relationships between fruit quality characteristics and leaf nutrient contents of cucumber plants. Dev Plant Soil Sci 86:193–195
• Yoshida S, Forno DA, Coc KJH, Gomex KA (1976) Laboratory manual for physiological studies of rice, 3rd edn. The International Rice Research Institute, Manila
• Zhang XZ (1994) Experimental Technology of Plant Physiology (in Chinese). Liaoning Science and Technology Press, Shenyang, pp 123–126

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