In vitro effect of leptin on anterior pituitary cells LH secretory activity during early pregnancy in pig

• Autorzy: Slawrys G. , Gajewska A.
• Języki publikacji: EN

Abstrakty

EN

Leptin modulates reproductive activity but its potential influence on LH secretion from anterior pituitary (AP) cells during implantation period in pigs (days 14-16 of pregnancy) remained unexplored. This study focused on determination whether leptin affects basal and GnRH-induced LH secretion and intracellular accumulation and whether leptin receptor (OB-Rb) mRNA is expressed in the AP gland during implantation in pigs. Four individual AP glands were developed into separate primary cultures. 2x10⁵ cells/ml were preincubated (72 h) and next, for 3.5 h, experimentally treated with GnRH (100 ng/ml), leptin (10⁻¹¹, 10⁻⁹, 10⁻⁷, 10⁻⁶ M) alone, or given in respective combinations with GnRH. In the AP gland, OB-Rb mRNA expression was determined by real-time PCR method. Leptin activated LH secretion and its concentration-dependent effect was observed as stimulation shown in a full range tested (culture 1) and exhibited only at 10⁻⁶ M (culture 2). A pooled data analysis revealed that basal LH secretion increased at 10⁻⁹, 10⁻⁷ and 10⁻⁶ M, but GnRH-induced LH release decreased at 10⁻⁶ M. Leptin down-regulated GnRH-induced LH secretion in all cultures, but only culture 3 exhibited sensitivity for all concentrations tested. Basal LH accumulation was activated in culture 1 (at 10⁻¹¹ M) and inhibited in culture 4 (at 10⁻⁹ M). In the presence of GnRH leptin up-regulated LH accumulation with individual culture leptin- ensitivity (culture 1-3), while down-regulated LH accumulation in culture 4. Obtained data indicate that OB- b mRNA is expressed in the AP gland and leptin alone and in combination with GnRH specifically modulates LH activity during early pregnancy in pigs.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2017
• Tom: 20
• Numer: 1
• Opis fizyczny: p.67-76,fig.,ref.

Twórcy

autor

Slawrys G.

• Department of Animal Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719 Olsztyn-Kortowo, Poland

autor

Gajewska A.

• Department of Neuroendocrinology, Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Science, 05-110 Jablonna n. Warsaw, Poland

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Identyfikatory

DOI

10.1515/pjvs-2017-0010