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2017 | 20 | 1 |

Tytuł artykułu

In vitro effect of leptin on anterior pituitary cells LH secretory activity during early pregnancy in pig

Treść / Zawartość

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Leptin modulates reproductive activity but its potential influence on LH secretion from anterior pituitary (AP) cells during implantation period in pigs (days 14-16 of pregnancy) remained unexplored. This study focused on determination whether leptin affects basal and GnRH-induced LH secretion and intracellular accumulation and whether leptin receptor (OB-Rb) mRNA is expressed in the AP gland during implantation in pigs. Four individual AP glands were developed into separate primary cultures. 2x10⁵ cells/ml were preincubated (72 h) and next, for 3.5 h, experimentally treated with GnRH (100 ng/ml), leptin (10⁻¹¹, 10⁻⁹, 10⁻⁷, 10⁻⁶ M) alone, or given in respective combinations with GnRH. In the AP gland, OB-Rb mRNA expression was determined by real-time PCR method. Leptin activated LH secretion and its concentration-dependent effect was observed as stimulation shown in a full range tested (culture 1) and exhibited only at 10⁻⁶ M (culture 2). A pooled data analysis revealed that basal LH secretion increased at 10⁻⁹, 10⁻⁷ and 10⁻⁶ M, but GnRH-induced LH release decreased at 10⁻⁶ M. Leptin down-regulated GnRH-induced LH secretion in all cultures, but only culture 3 exhibited sensitivity for all concentrations tested. Basal LH accumulation was activated in culture 1 (at 10⁻¹¹ M) and inhibited in culture 4 (at 10⁻⁹ M). In the presence of GnRH leptin up-regulated LH accumulation with individual culture leptin- ensitivity (culture 1-3), while down-regulated LH accumulation in culture 4. Obtained data indicate that OB- b mRNA is expressed in the AP gland and leptin alone and in combination with GnRH specifically modulates LH activity during early pregnancy in pigs.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

20

Numer

1

Opis fizyczny

p.67-76,fig.,ref.

Twórcy

autor
  • Department of Animal Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719 Olsztyn-Kortowo, Poland
autor
  • Department of Neuroendocrinology, Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Science, 05-110 Jablonna n. Warsaw, Poland

Bibliografia

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Typ dokumentu

Bibliografia

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