An efficient regeneration system via direct and indirect organogenesis for the medicinal plant Dysosma versipellis (Hance) M.Cheng and its potential as a podophyllotoxin source

• Autorzy: Jiang W. , Chen L. , Pan Q. , Qiu Y. , Shen Y. , Fu C.
• Języki publikacji: EN

Abstrakty

EN

Dysosma versipellis (Hance) M. Cheng is an endangered plant due to overharvesting for the extraction of podophyllotoxin. Thus, the in vitro technique is valuable for the propagation of this species. When the explants of rhizome buds were cultured on Murashige and Skoog’s (MS) medium with 6-benzyladenine (BA) (1.0 mg l⁻¹), gibberellic acid (GA₃) (0.5 mg l⁻¹) and zeatin (Zea) (0.5 mg l⁻¹), multiple buds were regenerated directly on the explants without callusing within 6 weeks. Callus was induced from the leaf segment cultures on MS basal medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) (0.5 mg l⁻¹) and BA (0.2 mg l⁻¹) within 4 weeks. The adventitious buds were differentiated when the calli were subcultured on MS medium supplemented with BA (1.0 mg l⁻¹) and thidiazuron (TDZ) (0.2 mg l⁻¹) within 6 weeks. The adventitious buds obtained from callus and the rhizome-buds rooted with a frequency of 100% on half strength MS medium fortified with indole-3-butyric acid (IBA) 0.5 mg l⁻¹ and activated charcoal (AC) 0.5 g l⁻¹ for 4 weeks. The rooted shoots were successfully transplanted from a mixture of vermiculite:soil (1:1 v/v) to the field with a survival rate of 85%. Podophyllotoxin production in calli, cultured rhizomes, rhizomes of transplanting plants from the garden and rhizomes in the wild field was confirmed by high-performance liquid chromatography (HPLC) analysis. Our results suggest that calli, cultured rhizomes and rhizomes of transplanting plants would be the potential sources of podophyllotoxin.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2012
• Tom: 34
• Numer: 2
• Opis fizyczny: p.631-639,fig.,ref.

Twórcy

autor

Jiang W.

• The Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education, College of Life Sciences, Zhejiang University, 310058 Hangzhou, People's Republic of China

autor

Chen L.

• The Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education, College of Life Sciences, Zhejiang University, 310058 Hangzhou, People's Republic of China

autor

Pan Q.

• The Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education, College of Life Sciences, Zhejiang University, 310058 Hangzhou, People's Republic of China

autor

Qiu Y.

• The Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education, College of Life Sciences, Zhejiang University, 310058 Hangzhou, People's Republic of China

autor

Shen Y.

• The Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education, College of Life Sciences, Zhejiang University, 310058 Hangzhou, People's Republic of China

autor

Fu C.

• The Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education, College of Life Sciences, Zhejiang University, 310058 Hangzhou, People's Republic of China

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