Synthesis of galactosyl mannitol derivative using β-Galactosidase from kluyveromyces lactisr

• Autorzy: Klewicki R. , Belina I. , Wojciechowska A. , Klewicka E. , Sojka M.
• Języki publikacji: EN

Abstrakty

EN

The purpose of the study was to identify the Influence of reactive mixture concentration (23–48 g/100 mL), pH (6.5–9.0), presence of NaCl (0.05–0.25 mol/L) and enzyme dose (2850–28,500 LAU/100 g of lactose) on the synthesis of galactosyl mannitol derivative using β-galactosidase from Kluyveromyces lactis. The use of the enzyme dose ranging from 2850 to 11,400 LAU/100 g lactose allowed obtaining gal-mannitol at the level of 21.8% total saccharides; higher doses intensified product decomposition. An increase in the concentration of the reactive mixture had a positive impact on the quantity of gal-mannitol obtained every single time, i.e. 4.39 g were obtained from 100 mL of a 23 g/100 mL solution and over 10 g were obtained from a 48 g/100 mL solution. A relatively low increase in product quantity (by ca. 5%) occurred after the pH was increased from 6.5 to 9.0. The use of NaCl rendered better results. An increase in the maximum content of gal-mannitol in the total sugar by 12.8% was observed at the concentration of 0.25 mol/L.

• Wydawca: -
• Czasopismo: Polish Journal of Food and Nutrition Sciences
• Rocznik: 2017
• Tom: 67
• Numer: 1
• Opis fizyczny: p.33-39,fig.,ref.

Twórcy

autor

Klewicki R.

• Institute of Food Technology and Analysis, Lodz University of Technology, Stefanowskiego 4/10, 90–924 Lodz, Poland

autor

Belina I.

• Institute of Food Technology and Analysis, Lodz University of Technology, Stefanowskiego 4/10, 90–924 Lodz, Poland

autor

Wojciechowska A.

• Institute of Food Technology and Analysis, Lodz University of Technology, Stefanowskiego 4/10, 90–924 Lodz, Poland

autor

Klewicka E.

• Institute of Fermentation Technology and Microbiology, Lodz University of Technology, Wolczanska 171/173, 90–924 Łodz, Poland

autor

Sojka M.

• Institute of Food Technology and Analysis, Lodz University of Technology, Stefanowskiego 4/10, 90–924 Lodz, Poland

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Identyfikatory

DOI

10.1515/pjfns-2016-0002