Catalytic assays in blue native gel revealed normal ATPasa but deficient NADH dehydrogenase activity in ZidaoA CMS line of rice (Oryza sativa)

• Autorzy: Yan Z. , Shao J. , Ding Y.
• Języki publikacji: EN

Abstrakty

EN

Cytoplasmic male sterility (CMS) is an important trait in rice (Oryza sativa L.) breeding because it provides a source for producing hybrid seeds. In rice CMS lines, ATPases involved in the oxidative phosphorylation complexes are believed to be dysfunctional due to the expression of rice CMS-related gene orf79. In the present study, a new type of CMS line named CMS-ZA (ZidaoA) was used. We found an orf79 homologous gene (named orfZ79) in three different rice lines (a CMS line, a maintainer line, and a hybrid). However, no detectable expression products of orfZ79 were found in the three lines. We evaluated the ATPase and NADH dehydrogenase activities of the three lines using in-gel catalytic assays. Our results show that the sterile line has intact ATPase activity, while NADH DHase activity is clearly decreased. To investigate NADH dehydrogenase deficiency, we measured NADH DHase activity in etiolated seedlings and green seedlings from the ZidaoA CMS sterile line and its corresponding maintainer line. We note that the NADH DHase activity of the sterile line was more deficient in green seedlings than that in etiolated seedlings. Our results show a possible role of NADH DHase deficiency to cause rice CMS.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2011
• Tom: 33
• Numer: 6
• Opis fizyczny: p.2477-2484,fig.,ref.

Twórcy

autor

Yan Z.

• State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, Hubei, People's Republic of China

autor

Shao J.

• State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, Hubei, People's Republic of China

autor

Ding Y.

• State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, Hubei, People's Republic of China

Bibliografia

• Akagi H, Sakamoto M, Shinjyo C, Shimada H, Fujimura T (1994) A unique sequence located downstream from the rice mitochondrial atp6 may cause male sterility. Curr Genet 25:52–58
• Chase CD (2006) Cytoplasmic male sterility: a window to the world of plant mitochondrial–nuclear interactions. Trends Genet 23:81–90
• Coster RV, Smet J, George E, Meirleir LD, Seneca S, Hove JV, Sebire G, Verhelst H, Bleecker JD, Vlem BV, Verloo P, Leroy J (2001) Blue native polyacrylamide gel electrophoresis: a powerful tool in diagnosis of oxidative phosphorylation defects. Pediatr Res 50:658–665
• Duan S, Li S, Li Y, Xiong Y, Zhu Y (2007) Distribution and SNPs of the rice CMS-related gene in AA-genome of Oryza species. Yi Chuan 29:455–461
• Duroc Y, Gaillard C, Hiard S, Tinchant C, Berthome R, Pelletier G, Budar F (2006) Nuclear expression of a cytoplasmic male sterility gene modifies mitochondrial morphology in yeast and plant cells. Plant Sci 170:755–767
• Dwivedi S, Perotti E, Ortiz R (2008) Towards molecular breeding of reproductive traits in cereal crops. Plant Biotechnol J 6:529–559
• Hanson MR (1991) Plant mitochondrial mutations and male sterility. Annu Rev Genet 25:461–486
• Hanson MR, Bentolila S (2004) Interactions of mitochondrial and nuclear genes that affect male gametophyte development. Plant Cell 16:S154–S169
• Heazlewood JL, Howell KA, Whelan J, Millar AH (2003) Towards an analysis of the rice mitochondrial proteome. Plant Physiol 132:230–242
• Hu Y, Wu Q, Liu S, Wei L, Chen X, Yan Z, Yu J, Zeng L, Ding Y (2005) Study of rice pollen grains by multispectral imaging microscopy. Microsc Res Tech 68:335–346
• Itabashi E, Kazama T, Toriyama K (2009) Characterization of cytoplasmic male sterility of rice with lead rice cytoplasm in comparison with that with Chinsurah Boro II cytoplasm. Plant Cell Rep 28:233–239
• Jung C, Higgins CM, Xu Z (2000) Measuring the quantity and activity of mitochondrial electron transport chain complexes in tissues of central nervous system using blue native polyacrylamide gel electrophoresis. Anal Biochem 286:214–223
• Juszczuk IM, Rychter AM (2009) BN-PAGE analysis of the respiratory chain complexes in mitochondria of cucumber MSC16 mutant. Plant Physiol Biochem 47:397–406
• Kazama T, Nakamura T, Watanabe M, Sugita M, Toriyama K (2008) Suppression mechanism of mitochondrial ORF79 accumulation by Rf1 protein in BT-type cytoplasmic male sterile rice. Plant J 55:619–628
• Krause F, Reifschneider NH, Vocke D, Seelert H, Rexroth S, Dencher NA (2004) ‘‘Respirasome’’-like supercomplexes in green leaf mitochondria of spinach. J Biol Chem 279:48369–48375
• Li S, Tan Y, Wang K, Wan C, Zhu Y (2008) Gametophytically alloplasmic CMS line of rice (Oryza sativa L.) with variant orfH79 haplotype corresponds to specific fertility restorer. Theor Appl Genet 117:1389–1397
• Marienfeld JR, Newton KJ (1994) The maize NCS2 abnormal growth mutant has a chimeric nad4–nad7 mitochondrial gene and is associated with reduced complex I function. Genetics 138:855–863
• Møller IM (2001) Plant mitochondria and oxidative stress: electron transport, NADPH turnover, and metabolism of reactive oxygen species. Annu Rev Plant Physiol Plant Mol Biol 52:561–591
• Møller IM (2002) A new dawn for plant mitochondrial NAD(P)H dehydrogenases. Trends Plant Sci 7:235–237
• Peng X, Li F, Li S, Zhu Y (2009) Expression of a mitochondrial gene orfH79 from the CMS-HongLian rice inhibits Saccharomyces cerevisiae growth and causes excessive ROS accumulation and decrease in ATP. Biotechnol Lett 31(3):409–414
• Rasmusson AG, Heiser VV, Zabaleta E, Brennicke A, Grohmann L (1998) Physiological, biochemical and molecular aspects of mitochondrial complex I in plants. Biochim Biophys Acta 1364:101–111
• Sabar M, Paepe RD, Kouchkovsky YD (2000) Complex I impairment, respiratory compensations, and photosynthetic decrease in nuclear and mitochondrial male sterile mutants of Nicotiana sylvestris. Plant Physiol 124:1239–1249
• Sabar M, Gagliardi D, Balk J (2003) ORFB is a subunit of F1F0-ATP synthase: insight into the basis of cytoplasmic male sterility in sunflower. EMBO Rep 4:381–386
• Sabar M, Janneke B, Leaver CJ (2005) Histochemical staining and quantification of plant mitochondrial respiratory chain complexes using blue-native polyacrylamide gel electrophoresis. The Plant J 44:893–901
• Schägger H, von Jagow G (1991) Blue native electrophoresis for isolation of membrane protein complexes in enzymatically active form. Anal Biochem 199:223–231
• Triepels RH, Van Den Heuvel LP, Trijbels JM, Smeitink JA (2001) Respiratory chain complex I deficiency. Am J Med Genet 106:37–45
• Wang Z, Zou Y, Li X, Zhang Q, Chen L, Wu H, Su D, Chen Y, Guo J, Luo D, Long Y, Zhong Y, Liu Y (2006) Cytoplasmic male sterility of rice with Boro II cytoplasm is caused by a cytotoxic peptide and is restored by two related PPR motif genes via distinct modes of mRNA silencing. Plant Cell 18:676–687
• Warmke HE, Lee SL (1978) Pollen abortion in T cytoplasm malesterile corn (Zea mays): a suggested mechanism. Science 200:561–563
• Wei L, Yan Z, Ding Y (2008) Mitochondrial RNA editing of F₀-ATPase subunit 9 gene (atp9) transcripts of Yunnan purple rice cytoplasmic male sterile line and its maintainer line. Acta Physiol Plant 30:657–662
• Wittig I, Karas M, Schägger H (2007) High resolution clear native electrophoresis for in-gel functional assays and fluorescence studies of membrane protein complexes. Mol Cell Proteomics 6:1215–1225
• Yi P, Yu T, Liu Y, Zhu Y (2004) Genomic context of nucleus influences mitochondrial gene expression of HL-type rice. Yi Chuan 26:186–188
• Zerbetto E, Vergani L, Dabbeni-Sala F (1997) Quantification of muscle mitochondrial oxidative phosphorylation enzymes via histochemical staining of blue native polyacrylamide gels. Electrophoresis 18:2059–2064
• Zhang H, Li S, Yi P, Wan C, Chen Z, Zhu Y (2007) A Honglian CMS line of rice displays aberrant F₀ of F₀F₁–ATPase. Plant Cell Rep 26:1065–1071
• Zhu Y (2000) Biology of the male sterility in rice, 1st edn. Wuhan University Press, Wuhan, China

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