Evaluation of migration of bone marrow-derived macrophages and limphocytes towards glioma cells and its modulation by cyclosporin A

• Autorzy: Sielska M. , Gabrusiewicz K. , Kwiatkowska E. , Kowalczyk D. , Ellert-Miklaszewska A. , Kaminska B.
• Języki publikacji: EN

Abstrakty

EN

Malignant glioblastomas are characterized by infi ltration of tumour tissue with brain macrophages that may consist up to 30% of tumour mass and contribute to tumour progression. A relative contribution of resident microglia and peripheral monocyte/macrophages in gliomas is poorly defi ned. We generated chimeric mice with the immune system reconstituted after irradiation with hematopoietic GFP-bone marrow cells. The dsRed-GL261 glioma cells were implanted to the brains of 16-weeks old C57BL/6 chimeric mice Two weeks after implantation, tumour bearing hemispheres were isolated and the number of CD11b+ CD45low microglial cells or CD11b+ CD45high macrophages was determined by fl ow cytometry. We found that peripheral GFP+ macrophages comprise above 60% of GFP+ cells in the tumor. A co-localization of Iba-1+ cells (macrophages/microglia) with GFP+ cells has been detected by confocal microscopy. Tumor associated peripheral macrophages can facilitate glioma invasion and promote angiogenesis. We have previously demonstrated that cyclosporin A (CsA) blocks activation of microglia and its promoting effects on glioma invasion in vitro and in vivo (Sliwa et al. 2007). In chimeric mice treated CsA, a percentage of GFP+ macrophages in gliomas was reduced suggesting an inhibitory effects of CsA on immune cell migration. Our studies demonstrate that blood-borne macrophages migrate to the tumour and consist a signifi cant population of tumour-associated macrophages.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2009
• Tom: 69
• Numer: 3
• Opis fizyczny: p.346

Twórcy

autor

Sielska M.

• Department of Cell Biology, Nencki Institute of Experimental Biology Polish Academy of Sciences, Warsaw, Poland

autor

Gabrusiewicz K.

• Department of Cell Biology, Nencki Institute of Experimental Biology Polish Academy of Sciences, Warsaw, Poland

autor

Kwiatkowska E.

• Department of Cancer Diagnostic and Immunology, The Great Poland Cancer Center, Poznan, Poland

autor

Kowalczyk D.

• Department of Cancer Diagnostic and Immunology, The Great Poland Cancer Center, Poznan, Poland

autor

Ellert-Miklaszewska A.

• Department of Cell Biology, Nencki Institute of Experimental Biology Polish Academy of Sciences, Warsaw, Poland

autor

Kaminska B.

• Department of Cell Biology, Nencki Institute of Experimental Biology Polish Academy of Sciences, Warsaw, Poland