Adequate S supply protects barley plants from adverse effects of salinity stress by increasing thiol contents

• Autorzy: Astolfi S. , Zuchi S.
• Języki publikacji: EN

Abstrakty

EN

As the salt-affected areas are expected to increase substantially in subsequent years, the impact of salinity on plant growth and yield is likely to increase. One of the first consequences of plant exposure to high saline concentrations is the formation of reactive oxygen species (ROS). In order to allow adjustment of the cellular redox state, plant antioxidative system has to be activated. This system involves several enzymes and compounds, as the sulphur-containing metabolite glutathione (GSH). Therefore, our aim was to determine whether adequate sulphur nutrition might alleviate the adverse effects of salt stress on barley plants grown in the presence of different sulphate application rate and exposed to 100 mM NaCl, by studying differences in growth parameters, lipid peroxidation, sulphate and thiol accumulation and sulphur assimilation pathway. In salt-treated plants, an adequate sulphur supply allows adequate GSH synthesis (high-thiol concentration) thus avoiding the effects of ROS on photosynthetic functions (no effect on both chlorophyll and protein content), whereas in S-deficient plants, salt stress leads to excess ROS production that induces stress and plants showed reduction of photosynthetic efficiency (loss of chlorophyll and protein contents). As thiol levels are more abundant in S-sufficient plants than in those S-deficient, one might expect that S-sufficient plants are more able to remove the harmful effects of high salinity. The comparison of malondialdehyde levels between +S and −S salt-treated plants strongly supports this idea. In conclusion, we found that plant sulphur nutritional status plays a key role in the metabolic modifications necessary to cope with salt stress.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2013
• Tom: 35
• Numer: 01
• Opis fizyczny: p.175-181,fig.,ref.

Twórcy

autor

Astolfi S.

• Department of Science and Technologies for Agriculture, Forestry, Nature and Energy (DAFNE), University of Viterbo, via S. C. de Lellis snc, 01100 Viterbo, Italy

autor

Zuchi S.

• Department of Science and Technologies for Agriculture, Forestry, Nature and Energy (DAFNE), University of Viterbo, via S. C. de Lellis snc, 01100 Viterbo, Italy

Bibliografia

• Astolfi S, Zuchi S, Cesco S, Varanini Z, Pinton R (2004) Influence of iron nutrition on sulphur uptake and metabolism in maize (Zea mays L.) eoots. Soil Sci Plant Nutr 50(7):1079–1083
• Astolfi S, Zuchi S, Passera C (2005) Effect of cadmium on H?ATPase activity of plasma membrane vesicles isolated from roots of different S-supplied maize (Zea mays L.) plants. Plant Sci 169:361–368. doi:10.1016/j.plantsci.2005.03.025
• Astolfi S, Zuchi S, Cesco S, Sanita` di Toppi L, Pirazzi D, Badiani M, Varanini Z, Pinton R (2006) Fe deficiency induces sulphate uptake and modulates redistribution of reduced sulphur pool in barley plants. Func Plant Biol 33:1055–1061
• Bardsley CE, Lancaster JD (1962) Determination of reserve sulphur and soluble sulphate in soils. Soil Sci Soc Am Proc 24:265–268
• Bradford MM (1976) A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein dye-binding. Anal Biochem 72:248–254
• Davidian JC, Kopriva S (2010) Regulation of sulfate uptake and assimilation—the same or not the same? Mol Plant 3(2):314–325
• Dluzniewska P, Gessler A, Dietrich H, Schnitzler J-P, Teuber M, Rennenberg H (2007) Nitrogen uptake and metabolism in Populus 9 canescens as affected by salinity. New Phytol 173: 279–293
• Epstein E, Norlyn JD, Rush DW, Kingsbury RW (1980) Saline culture of crops: a genetic approach. Science 210:399–404
• Fei G, YiJun Z, LingYun H, DaCheng HE, GenFa Z (2008) Proteomic analysis of long-term salinity stress responsive proteins in Thellungiella halophila leaves. Chinese Sci Bull 53:3530–3537
• Foyer CH, Noctor G (2003) Redox sensing and signalling associated with reactive oxygen in chloroplasts, peroxisomes and mitochondria. Physiol Plant 119:355–364
• Foyer CH, Noctor G (2005) Redox homeostasis and antioxidant signaling: a metabolic interface between stress perception and physiological responses. Plant Cell 17:1866–1875
• Haller E, Suter M, Brunold C (1986) Regulation of ATP-sulfurylase and adenosine 50-phosphosulfate sulfotransferase by the sulfur and the nitrogen source in heterotrophic cell suspension cultures of Paul’s scarlet rose. J Plant Physiol 125:275–286
• Hell R, Bergmann L (1990) c-Glutamylcysteine synthetase in higher plants: catalytic properties and subcellular localization. Planta 180:603–612
• Hesse H, Nikiforova V, Gakiere B, Hoefgen R (2004) Molecular analysis and control of cysteine biosynthesis: integration of nitrogen and sulphur metabolism. J Exp Bot 55(401):1283–1292
• Kato M, Shimizu S (1985) Chlorophyll metabolism in higher plants VI. Involvement of peroxidase in chlorophyll degradation. Plant Cell Physiol 26:1291–1301
• Khan NA, Anjum NA, Nazar R, Iqbal N (2009a) Increased activity of ATPsulfurylase, contents of cysteine and glutathione reduce high cadmium-induced oxidative stress in high photosynthetic potential mustard (Brassica juncea L.) cultivar. Russ J Plant Physiol 56:670–677
• Khan NA, Nazar R, Anjum NA (2009b) Growth, photosynthesis and antioxidant metabolism in mustard (Brassica juncea L.) cultivars differing in ATP-sulfurylase activity under salinity stress. Sci Hort 122:455–460
• Koprivova A, Kopriva S (2008) Lessons from investigation of regulation of APS reductase by salt stress. Plant Signal Behav 8:567–569
• Koprivova A, North KA, Kopriva S (2008) Complex signaling network in regulation of adenosine 5-phosphosulfate reductase by salt stress in Arabidopsis roots. Plant Physiol 146:1408–1420
• Lopez-Berenguera C, Carvajala M, Garcea-Viguerab C, Alcaraz CF (2007) Nitrogen, phosphorus, and sulfur nutrition in Broccoli plants grown under salinity. J Plant Nutr 30:1855–1870
• Mahajan S, Tuteja N (2005) Cold, salinity and drought stresses: an overview. Arch Biochem Biophys 444:139–158
• Manchanda G, Garg N (2008) Salinity and its effect on the functional biology of legumes. Acta Physiol Plant 30:595–618
• Miller G, Suzuki N, Ciftci-Yilmazi N, Mittler R (2010) Reactive oxygen species homeostasis and signaling during drought and salinity stresses. Plant Cell Environ 33:453–467
• Mittova V, Theodoulou FL, Kiddle G, Go´mez L, Volokita M, Tal M, Foyer C, Guy M (2003) Coordinate induction of glutathione biosynthesis and glutathione-metabolizing enzymes is correlated with salt tolerance in tomato. FEBS Lett 554:417–421
• Nazar R, Iqbal N, Syeed S, Khan NA (2011) Salicylic acid alleviates decreases in photosynthesis under salt stress by enhancing nitrogen and sulfur assimilation and antioxidant metabolism differentially in two mungbean cultivars. J Plant Physiol 168: 807–815
• Noctor G, Foyer CH (1998) Ascorbate and glutathione: keeping active oxygen under control. Annu Rev Plant Physiol Plant Mol Biol 49:249–279
• Pilon-Smits EAH, Hwang S, Lytle CM, Zhu Y, Tai JC, Bravo RC, Chen Y, Leustek T, Terry N (1999) Overexpression of ATP sulfurylase in Indian mustard leads to increased selenate uptake, reduction and tolerance. Plant Physiol 119:123–132
• Rennenberg H, Brunold C (1994) Significance of glutathione in plants under stress. Progr Bot 55:142–156
• Reuveny Z, Dougall DK, Trinity PM (1980) Regulatory coupling of nitrate and sulfate assimilation pathways in cultured tobacco cells. Proc Natl Acad Sci USA 77:6670–6672
• Robinson D (1994) The responses of plants to non-uniform supplies of nutrients. New Phytol 127:635–674
• Ruiz JM, Blumwald E (2002) Salinity-induced glutathione synthesis in Brassica napus. Planta 214:965–969
• Saito K (2000) Regulation of sulphate transport and synthesis of sulphur-containing amino acids. Curr Opin Plant Biol 3:188–195
• Schmutz D, Brunold C (1982) Rapid and simple measurement of ATP sulphurylase activity in crude plant extracts using an ATP meter for bioluminescence determination. Anal Biochem 121:151–155
• Zhang FS, Ro¨mheld V, Marschner H (1991) Role of the root apoplasm for iron acquisition by wheat plants. Plant Physiol 97:1302–1305
• Zhao FJ, Hawkesford MJ, McGrath SP (1999) Sulphur assimilation and effects on yield and quality of wheat. J Cereal Sci 30:1–17
• Zhu J, Fu X, Koo YD, Zhu JK et al (2007) An enhancer mutant of Arabidopsis salt overly sensitive 3 mediates both ion homeostasis and the oxidative stress response. Mol Cell Biol 27:5214–5224
• Zuchi S, Cesco S, Astolfi S (2012) High S supply improves Fe accumulation in durum wheat plants grown under Fe limitation. Env Exp Bot 77:25–32. doi:10.1016/j.envexpbot.2011.11.001

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