Spinal cord injury or enhanced physical activity stimulate division of ependymal cells rat spinal cord

• Autorzy: Novotna I. , Slovinska L. , Cizek M. , Nagyova M. , Rosocha J. , Radonak J. , Vanicky I. , Cizkowa D.
• Języki publikacji: EN

Abstrakty

EN

Ependymal cells (EC) in the spinal cord central canal (CC) are believed to be responsible for the postnatal neurogenesis following pathological or stimulatory conditions. In the present study we have analyzed the proliferation of the CC EC in adult rats processed to spinal cord injury (SCI) or enhanced physical activity. To label dividing cells, a daily injection of Bromodeoxyuridine (BrdU) was administered over a 14 days. Quantifi cation of BrdU positive EC was performed by using stereological principles of systematic random sampling and optical Dissector software. The number of BrdU labeled EC increased gradually with the time of survival after both paradigms, SCI or increased physical activity. In the SCI group we have found 8.2-fold (7 days) and 11.3-fold (14 days) increase of proliferating EC in the rostro-caudal regions. Furthermore, the cervical spinal cord segments revealed 2 to 3-fold increase of EC for both time-points analyzed. In the second group subjected to enhanced physical activity by running wheel, we have observed 1.8 fold increase of dividing EC in the lumbar and 3.2 fold increase in the cervical spinal cord segments at 7 days, but no signifi cant progression at 14 days. This data shows, that SCI or enhanced physical activity in adult rats induces an endogenous EC response leading to their increased proliferation, which may be benefi cial for recovery of motor function. Supported by: APVV 51-002105, VEGA 2-0019-08, VEGA 1-0674-09, VEGA 1/4223/07, APVV SK-CZ-0045-07.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2009
• Tom: 69
• Numer: 3
• Opis fizyczny: p.293

Twórcy

autor

Novotna I.

• Institute of Neurobiology SAS, Kosice, Slovakia

autor

Slovinska L.

• Institute of Neurobiology SAS, Kosice, Slovakia

autor

Cizek M.

• Department of Infections Diseases, University of Veterinary Medicine, Kosice, Slovakia

autor

Nagyova M.

• Institute of Neurobiology SAS, Kosice, Slovakia

autor

Rosocha J.

• Associated Tissue Cultute Bank, Medical Faculty, Kosice, Slovakia

autor

Radonak J.

• II Surgical Clinic, University of PJ Safarik, Kosice, Slovakia

autor

Vanicky I.

• Institute of Neurobiology SAS, Kosice, Slovakia

autor

Cizkowa D.

• Institute of Neurobiology SAS, Kosice, Slovakia