Effect of injected position of cultured cumulus cells as donor cells on reconstructed in vitro matured porcine oocytes

• Autorzy: Lin Y.-A. , Tsai H.-B. , Dybus A. , Chen M.-C. , Liao M.-H.
• Języki publikacji: EN

Abstrakty

EN

The effect was studied of injected position of cultured cumulus cells as donor cells for nuclear transfer on the process of in vitro matured reconstructed porcine oocytes. The cumulus-oocyte complexes (COCs) obtained from ovaries were cultured in NCSU-23 maturation medium. The cumulus cells were separated from matured COCs, and cultured in DMEM medium (10% fetal calf serum,FCS) for up to 4 passages. The cultured cumulus cells were injected into the enucleated oocytes to obtain the reconstructed oocytes. The chosen positions were the perivitelline space, ooplasm edge and ooplasm center. After nuclear transfer, the reconstructed oocytes were fused and activated simultaneously. Then, the activated oocytes were cultured in NCSU-23 embryo culture medium for 16 h to observe the activation, or 144 h to observe the embryo development. It was found that the injected position affected the activation rate, pronuclear formation rate and embryo development rate of the reconstructed oocytes significantly. The activation rate, pronuclear formation rate and embryo development rate of the ooplasm edge group were obviously greater than the perivitelline space and ooplasm center groups. Furthermore, the injected position at the ooplasm edge favoured the more advanced development. The ooplasm edge would be an optimum injected position.

• Wydawca: -
• Czasopismo: Animal Science Papers and Reports
• Rocznik: 2010
• Tom: 28
• Numer: 3
• Opis fizyczny: p.281-290,fig.,ref.

Twórcy

autor

Lin Y.-A.

• Department of Tropical Agriculture and International Cooperation, National Pingtung University of Science and Technology, Pingtung, Taiwan, Republic of China
• Department of Animal Science, National Ilan University, I-Lan, Taiwan, Republic of China

autor

Tsai H.-B.

• Department of Chemical and Materials Engineering, National Ilan University, I-Lan, Taiwan, Republic of China

autor

Dybus A.

• Laboratory of Molecular Cytogenetics, Faculty of Biotechnology and Animal Science, Agricultural University of Szczecin, 71-460 Szczecin, Poland

autor

Chen M.-C.

• Department of Animal Science, National Ilan University, I-Lan, Taiwan, Republic of China

autor

Liao M.-H.

• Department of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung, Taiwan, Republic of China

Bibliografia

• ABEYDEERA L.R., WANG W.H., CANTLEY T.C., 1998 – Presence of epidermal growth factor during in vitro maturation of pig oocytes and embryo culture can modulate blastocyst development after in vitro fertilization. Molecular Reproduction and Development 51, 395-401.
• COSKUN S., LIN Y.C., 1994 – Effect of transforming growth factors and activation on in vitro porcine oocyte maturation. Molecular Reproduction and Development 38, 153-159.
• FUKUI Y., 1990 – Effect of follicular cells on the acrosome reaction, fertilization, and developmental competence of bovine oocytes matured in vitro. Molecular Reproduction and Development 26, 40-46.
• HUANG W.T., HOLTZ W., 1997 – Survival of porcine GV and MII-oocytes after exposure to cryoprotectants and cooling to 10o. Theriogenology 47, 346.
• HYUN S.H., LEE G.S., KIM D.Y., KIM H.S., LEE S.H., KIM S., LEE E.S., LIM J.M., KANG S.K.,LEE B.C., HWANG W.S., 2003 – Effect of maturation media and oocytes derived from sows or gilts on the development of cloned pig embryos. Theriogenology 59, 1641-1649.
• JANG G., OH H.J., FIBRIANTO Y.H., HOSSEIN M.S., KIM H.J., KIM J.J., HONG S.G., PARK J.E., KANG S.K., LEE B.C., 2008 – Improvement of canine somatic cell nuclear transfer procedure.Theriogenology 69, 146-154.
• KA H.H., SAWAI K., WANG W.H., IM K.S., NIWA K., 1997 – Amino acids in maturation medium and presence of cumulus cells at fertilization promote male pronuclear formation in porcine oocytes matured and penetrated in vitro. Biology of Reproduction 57, 1478-1483.
• KALOUS J., SUTOVSKY P., PIMKEVICOVA Z., SHIOYA Y., LIE B., MOLTIK J., 1993 – Pig membrane granulose cells prevent resumption of meiosis in cattle oocytes. Molecular Reproduction Development 34, 58-64.
• KITIYANANT Y., SAIKHUN J., PAVASUTHIPAISIT K., 2003 – Somatic cell nuclear transfer in domestic cat oocytes treated with IGF-1 for in vitro maturation. Theriogenology 59, 1775-1786.
• KUBOTA C., YAMAKUCHI H., TODORAKI J., MIZOSHITA K., TABARA N., BARBER M. YANG X., 2000 – Six cloned calves produced from adult fibroblast cells after long term culture.Proceedings of the National Academy of Sciences of the USA 97, 990-995.
• KWON D.J., PARK C.K., YANG B.K., KIM C.I., CHEONG H.T., 2007 – Effects of maturational age of recipient oocytes and activation conditions on the development of porcine fetal fibroblast nuclear transfer embryos. Animal Reproduction Science 100, 211-215.
• LEE G.S., HYUN S.H., KIM H.S., KIM D.Y., LEE S.H., LIM J.M., LEE E.S., KANG S.K., LEE B.C., HWANG W.S., 2003 – Improvement of a porcine somatic cell nuclear transfer technique by optimizing donor cell and recipient oocyte preparations. Theriogenology 59, 1949-1957.
• LIN T.A., TSAY C., CHEN C.H., TANG P.C., JU J.C., 2008 – Nuclear and cytoskeletal dynamics during oocyte maturation and development of somatic cell cloned pig embryos injected with membrane disintegrated donor cells. Animal Reproduction Science 103, 107-119.
• LIU J.L., WANG M.K., SUN Q.Y., XU Z., CHEN D.Y., 2000 – Effect of telophase enucleation on bovine somatic nuclear transfer. Theriogenology 54, 989-998.
• MACHATY Z., DAY B.N., PRATHER R.S., 1998 – Development of early porcine embryos in vitro and in vivo. Biology of Reproduction 59, 451-455.
• MORI T., TOMOKO A., HIROSHI S., 2000 – Roles of gap junctional communication of cumulus cells in cytoplasmic maturation of porcine oocytes cultured in vitro. Biology of Reproduction 62,913-919.
• NAKAYAMA T., INOUE M., SATO E., 1996 – Effect of oocytectomy on glycosaminoglycan composition during cumulus expansion of porcine cumulus-oocyte complexes cultured in vitro.Biology of Reproduction 55, 1299-1304.
• SAS., 1999 – User’s Guide: Statistics. Release 8.02 Edition. SAS Institute Inc. Cary NC.
• SAIKHUN J., PAVASUTHIPAISIT K., JARUANSUWAN M., KITIYANANT Y., 2002 – Xenonuclear transplantation of buffalo (Bubalus bubalis) fetal and adult somatic cell nuclei into bovine (Bos indicus) oocyte cytoplasm and their subsequent development . Theriogenology 57, 1829-1837.
• TERLOUW S.L., PRATHER R.S., DAY B.N., 1992 – In vitro development of nuclear transplant pig embryos. Theriogenology 37, 309.
• UHM S.J., CHUNG H.M., KIM C., SHIM H., KIM N.H., LEE H.T., CHUNG K.S., 2000 – In vitro development of porcine enucleated oocytes reconstructed by the transfer of porcine fetal fibroblasts and cumulus cells. Theriogenology 54, 559-570.
• VANDERHYDEN B.C., ARMSTRONG D.T., 1989 – Role of cumulus cells and serum on the in vitro maturation, fertilization, and subsequent development of rat oocytes. Biology of Reproduction 40, 720-728.
• WAKAYAMA T., YANAGIMACHI R., 1999 – Cloning of male mice from adult tip tail cells. Nature Genetics 22, 127-128.
• WAKAYAMA T., PERRY A.C., ZUCCOTTI M., JOHNSON K.R., YANAGIMACHI R., 1998 –Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei. Nature 394, 369-374.
• WANG W.H., ABEYDEERA L.R., CANTLEY T.C., DAY B.N., 1997 – Effects of oocyte maturation media on development of pig embryos produced by in vitro fertilization. Journal of Reproduction and Fertility 111, 101-108.
• WELLS D.N., MISICA P.M., TERVIT H.R., 1999 – Production of cloned calves following nuclear transfer with cultured adult mural granulose cells. Biology of Reproduction 60, 996-1005.
• WILMUT I., SCHNIEKE A.E., MCWHIR J., KIND A.J., CAMPBELL K.H.S., 1997 – Viable offspring derived from fetal and adult mammalian cells. Nature 385, 810-813.
• ZAKHARTCHENKO V., HILLS G.D., SCHERNTHANER W., STOJKOVIC M. REICHENBACH H.D., MUELLER S., PRELLE K., STEINBORN R., MUELLER M., WOLF E., BREM G., 1999a –Potential of fetal germ cell for nuclear transfer in cattle. Molecular Reproduction and Development 52, 421-426.
• ZAKHARTCHENKO V., ALBERIO R., STOJKOVIC M., PRELLE K., SCHERNTHANER W.,STOJKOVIC P., WENIGERKIND H., WANEK R., DÜCHLER M., STEINBORN R., MUELLER M., BREM G., WOLF E., 1999b – Adult cloning in cattle: Potential of nuclei from a permanent cell line and from primary cultures. Molecular Reproduction and Development 54, 264-272.