Sucrose and cytokinin interactions in relation to ethylene and abscisic acid production in the regulation of morphogenesis in pelargonium x hortorum L.H. Bailey in vitro

• Autorzy: Wojtania A. , Wegrzynowicz-Lesiak E. , Dziurka M. , Waligorski P.
• Języki publikacji: EN

Abstrakty

EN

The aim of the study was to determine the effect of exogenous sucrose and cytokinin on ethylene production and responsiveness in relation to the shoot formation of Pelargonium × hortorum 'Bergpalais' in vitro. Increasing the concentration of sucrose from 15 to 40 g L-1 in medium containing meta-topolin (mT) resulted in a two-fold decrease in the number of shoots and leaves as well as a reduction in ethylene production. The addition of ethylene synthesis inhibitor (AVG) to mT-medium significantly reduced the ethylene production and the shoot growth, but it had no significant influence on the shoot formation. The mT-induced shoot formation was, however, significantly reduced in the presence of ethylene action inhibitor (AgNO3), in a manner dependent on sucrose levels. At the end of the subculture period, increased sucrose concentrations (15–40 g L-1) in the presence of mT and AgNO3 resulted in a 3.7-fold increase in ethylene emission. At the same time, the supply of sucrose caused a 2.8-fold increase in the level of endogenous abscisic acid (ABA). Our results may suggest that the inhibitory effect of high sucrose concentration (30 and 40 g L-1) may depend on its influence on ethylene sensitivity. It also suggests that sucrose-regulation of the shoot formation of Pelargonium in vitro is mediated by ABA.

Słowa kluczowe

EN

pelargonium; geranium; Ethylene inhibitor; abscisic acid; endogenous abscisic acid; sucrose concentration; shoot multiplication

• Wydawca: -
• Czasopismo: Acta Biologica Cracoviensia. Series Botanica
• Rocznik: 2015
• Tom: 57
• Numer: 1
• Opis fizyczny: p.62-69,fig.,ref.

Twórcy

autor

Wojtania A.

• Research Institute of Horticulture, Skierniewice, Poland

autor

Wegrzynowicz-Lesiak E.

• Research Institute of Horticulture, Skierniewice, Poland

autor

Dziurka M.

• F.Gorski Institute of Plant Physiology, Polish Academy of Sciences, Krakow, Poland

autor

Waligorski P.

• F.Gorski Institute of Plant Physiology, Polish Academy of Sciences, Krakow, Poland

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Identyfikatory

DOI

10.1515/abcsb-2015-0005