Chloroplast protease/chaperone AtDeg2 influences cotyledons opening and reproductive development in Arabidopsis

• Autorzy: Adamiec M. , Jagodzik P. , Wyka T.P. , Ludwikow A. , Mitula F. , Misztal L. , Lucinski R. , Jackowski G.
• Języki publikacji: EN

Abstrakty

EN

AtDeg2 is a chloroplast protein with dual protease/chaperone activity. Since data on how the individual activities of AtDeg2 affect growth and development of Arabidopsis thaliana plants is missing, two transgenic lines were prepared that express mutated AtDeg2 versions that have either only protease or chaperone activity and a comprehensive ontogenesis stage-based study was performed comprising wild type (WT) plants and insertional mutants that do not express AtDeg2, as well as the two transgenic lines. The repression of both AtDeg2 activities in deg2-3 mutants altered just a few phenotypic traits including the time when cotyledons were fully opened, the time when 10% flowers were open as well as the number of inflorescence branches and seed length in plants which have completed their generative development. It was demonstrated that complete opening of cotyledons as well as the number of inflorescence branches and seed length in plants which have completed their generative development required involvement of both AtDeg2 activities, whereas the time when 10% of flowers were open was controlled by AtDeg2 protease activity. These results show for the first time that the chaperone activity of AtDeg2 is needed for some elements of generative development of A. thaliana plants to proceed normally. So far, the chaperone activity of AtDeg2 was confirmed based on in vitro assays only.

• Wydawca: -
• Czasopismo: Acta Societatis Botanicorum Poloniae
• Rocznik: 2018
• Tom: 87
• Numer: 2
• Opis fizyczny: Article 3584 [13p.],fig.,ref.

Twórcy

autor

Adamiec M.

• Department of Plant Physiology, Institute of Experimental Biology, Adam Mickiewicz University in Poznan, Umultowska 89, 61-614 Poznan, Poland

autor

Jagodzik P.

• Department of Plant Physiology, Institute of Experimental Biology, Adam Mickiewicz University in Poznan, Umultowska 89, 61-614 Poznan, Poland

autor

Wyka T.P.

• Department of General Botany, Institute of Experimental Biology, Adam Mickiewicz University in Poznan, Umultowska 89, 61-614 Poznan, Poland

autor

Ludwikow A.

• Department of Biotechnology, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University in Poznan, Umultowska 89, 61-614 Poznan, Poland

autor

Mitula F.

• Department of Biotechnology, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University in Poznan, Umultowska 89, 61-614 Poznan, Poland

autor

Misztal L.

• Department of Plant Physiology, Institute of Experimental Biology, Adam Mickiewicz University in Poznan, Umultowska 89, 61-614 Poznan, Poland

autor

Lucinski R.

• Department of Plant Physiology, Institute of Experimental Biology, Adam Mickiewicz University in Poznan, Umultowska 89, 61-614 Poznan, Poland

autor

Jackowski G.

• Department of Plant Physiology, Institute of Experimental Biology, Adam Mickiewicz University in Poznan, Umultowska 89, 61-614 Poznan, Poland

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Identyfikatory

DOI

10.5586/asbp.3584