Improving an in vitro propagation protocol for Cestrum nocturnum L.

• Autorzy: Rasheed K.A.

Warianty tytułu

PL

Udoskonalenie metody rozmnażania Cestrum nocturnum L. w warunkach in vitro

• Języki publikacji: EN

Abstrakty

EN

The present study was carried out to assess the micropropagation of Cestrum (Cestrum nocturnum L.) by using single nodes and shoot tips excised from soft cuttings using MS salts, 30 g × l-1 sucrose, 7 g × l-1 agar, and different concentrations of plant growth regulators in culture medium. The results revealed that the use of mercuric chloride (0.05%, HgCl2) for 7 minutes was very effective in preventing contamination and gave the highest survival percentage (99%). The highest response (100%) was gained at initiation stage from lateral bud explants on MS medium supplemented with 1.5 mg × l-1 of BA with most of NAA concentrations. However, in case of terminal buds, higher percentages of responses were resulted from the interaction of BA (1.5 mg × l-1) with 0.2 mg × l-1 NAA. The lateral buds also produced more new shoots as well as a higher number of leaves and length of new shoots on the medium supplemented with 1.5 mg × l-1 BA as compared with those from terminal buds. Significant differences were observed at multiplication stage between the lateral buds and terminal buds, since the lateral buds produced a higher number of new shoots and leaves as well as longer new shoots. At rooting stage, the treatment with 1 mg × l-1 IBA gave the highest percentage of rooting (100%), the highest number of roots (13.2 root/explant), and the longest roots (8.44 cm), respectively, on half strength MS medium. Plantlets obtained were transferred to pots and acclimatized with 90% success.

PL

Badania przeprowadzono w celu oceny mikrorozmnażania Cestrum (Cestrum nocturnum L.) przy wykorzystaniu pojedynczych węzłów oraz wierzchołków pędów wyciętych z miękkich części roślin. Zastosowano sole MS, 30 g × l-1 sacharozy, 7 g × l-1 agaru oraz różne stężenia regulatorów wzrostu roślin w podłożu hodowlanym. Wyniki pokazały, że zastosowanie chlorku rtęci (0,05%, HgCl2) przez 7 minut było bardzo efektywne w usuwaniu zanieczyszczeń oraz dało najwyższy procent przeżywalności (99%). Najwyższy procent reakcji (100%) uzyskano w fazie inicjacji eksplantatów przypadku eksplantatów z pąków bocznych na pożywce MS uzupełnionej 1,5 mgl-1 BA i przy zastosowaniu większości stężeń NAA. Jednak w przypadku pąków wierzchołkowych wyższe procenty reakcji wynikały z interakcji BA (1.5 mg × l-1) z 0,2 mg × l-1 NAA. Pąki boczne również wytworzyły więcej nowych pędów, większą liczbę liści oraz nowe pędy o większej długości na pożywce z dodatkiem 1,5 mg × l-1 BA w porównaniu z pąkami wierzchołkowymi. Istotne różnice pomiędzy pąkami bocznymi i pąkami wierzchołkowymi obserwowano w fazie namnażania, ponieważ pąki boczne wytworzyły większą liczbę nowych pędów i liści oraz dłuższe nowe pędy. W fazie ukorzeniania zastosowanie 1 mg × l-1 IBA dało najwyższy procent ukorzenienia (100%) oraz najwyższą liczbę korzeni na explantat (13,2) i najdłuższe korzenie (8,44 cm) na pożywce MS z zestawu soli. Uzyskane mikrosadzonki zostały przeniesione do doniczek i aklimatyzowano je z 90% skutecznością.

• Wydawca: -
• Czasopismo: Acta Agrobotanica
• Rocznik: 2013
• Tom: 66
• Numer: 2
• Opis fizyczny: p.35-44,fig.,ref.

Twórcy

autor

Rasheed K.A.

• Department of Horticulture, Faculty of Agriculture and Forestry, University of Duhok, Kurdistan Region, Iraq

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