High frequency of doubled haploid plant production in spelt wheat

• Autorzy: Lantos C. , Jenes B. , Bona L. , Cserhati M. , Pauk J.
• Języki publikacji: EN

Abstrakty

EN

This is the first study to report an efficient anther culture (AC) method for spelt wheat, which has an increasing importance not only in applied research but also in organic farming and changing nutritional standards. In this study, an efficient AC protocol has been described for ‘GK Fehér’ spelt wheat. The number of AC-derived embryolike structures (ELS) was 62.2/100 anthers, from which we were able to regenerate 30.6 green plantlets per 100 anthers. The percentage of green plantlets production was 89.0% among the regenerated plantlets, while the phenomenon of albinism was restricted (3.8/100 anthers). Altogether, from AC of ‘GK Fehér’ 306 green plantlets were produced in vitro and 241 plants were acclimatized to the greenhouse conditions. Based on ploidy level analyses, 83 spontaneous doubled haploid (DH) plants were produced (8.3 DH plants/100 anthers), so the percentage of spontaneous rediploidization was 34.4%. The spontaneous DH plants produced fertile spikes, while a few seeds were harvested from seven partially fertile plants.

Słowa kluczowe

EN

spelt wheat; androgenesis; anther culture; haploid plant; double haploid; embryo-like structure

• Wydawca: -
• Czasopismo: Acta Biologica Cracoviensia. Series Botanica
• Rocznik: 2016
• Tom: 58
• Numer: 2
• Opis fizyczny: p.107-112,fig.,ref.

Twórcy

autor

Lantos C.

• Cereal Research Non-Profit Ltd., Department of Biotechnology, P.O. Box 391, H-6701 Szeged, Hungary

autor

Jenes B.

• Department of Plant Biotechnology, Plant Cell Biology Group, Agricultural Biotechnology Institute, Szent-Gyorgyi Albert u. 4, 2100 Godollo, Hungary

autor

Bona L.

• Cereal Research Non-Profit Ltd., Department of Minor Cereals, P.O. Box 391, H-6701 Szeged, Hungary

autor

Cserhati M.

• Department of Genetics, Cell Biology and Anatomy, University of Nebraska Medical Center, 42 nd and Emile street, Omaha, NE, 68198, USA

autor

Pauk J.

• Cereal Research Non-Profit Ltd., Department of Biotechnology, P.O. Box 391, H-6701 Szeged, Hungary

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Identyfikatory

DOI

10.1515/abcsb-2016-0014