Effect of selected divalent cations on protein mobilization in lentil (Lens culinaris) sprouts

• Autorzy: Swieca M. , Baraniak B. , Gawlik-Dziki U.

Warianty tytułu

PL

Wpływ wybranych jonów dwuwartościowych na mobilizację białek w kiełkach soczewicy jadalnej (Lens culinaris)

• Języki publikacji: EN

Abstrakty

EN

The influence of calcium and magnesium on protein mobilization and non-protein nitrogen content in cotyledons of germinated lentil was studied. Elicitation of metabolism in sprouts modifies the rate of protein mobilization and causes a subsequent increase in the non-protein nitrogen fraction in lentil cotyledons. Application of MgCl 2 proved to be the most beneficial, leading to the highest yield of non-protein nitrogen in cotyledons from 6-days-old lentil sprouts. Regardless of the type of elicitor, a decrease in the activity of proteolytic enzymes was observed in cotyledons from 3- and 4-day-old lentil sprouts. A significant correlation was found in sprouts elicited with MgCl 2 between the non-protein nitrogen content and activities of endo- and aminopeptidases. The information provided by this study allows us to design conditions for lentil sprouting which will result in an enhanced level of potentially bioaccessible non-protein nitrogen (free amino acids and peptides).

PL

• Wydawca: -
• Czasopismo: Journal of Elementology
• Rocznik: 2014
• Tom: 19
• Numer: 2
• Opis fizyczny: p.577-585,fig.,ref.

Twórcy

autor

Swieca M.

• Chair of Biochemistry and Food Chemistry, University of Life Sciences in Lublin, Skromna Str. 8, 20-704 Lublin, Poland

autor

Baraniak B.

• Chair of Biochemistry and Food Chemistry, University of Life Sciences in Lublin, Lublin, Poland

autor

Gawlik-Dziki U.

• Chair of Biochemistry and Food Chemistry, University of Life Sciences in Lublin, Lublin, Poland

Bibliografia

• Anson M.L. 1938. The estimation of pepsin, trypsin, papain, and cathepsin with hemoglobin. J. Gen. Physiol., 22: 79-89.
• Antão C.M., Malcata X. 2005. Plant serine proteases: biochemical, physiological and molecular features. Plant Physiol. Biochem., 43(7): 637-650.
• Domash V.I., Sharpio T.P., Zabreiko S.A., Sosnovskaya T.F. 2008. Proteolytic enzymes and trypsin inhibitors of higher plants under stress conditions. Russ. J. Bioorg. Chem., 34 (3): 318-322.
• Gawlik-Dziki U., Świeca M. 2011. Sprouts of selected plants as a source of bioavailable antioxidants and lipoxygenase inhibitors. Ann. UMCS Lublin, Sect., DDD., 23, 3(17): 161-168.
• Gawlik-Dziki U., Świeca M., Sug ier D. 2012. Enhancement of antioxidant abilities and the lipoxygenase and xanthine oxidase inhibitory activity of broccoli sprouts by biotic elicitors. Acta Sci. Pol., Hort. Cult., 11(1): 13-25.
• Ghavidel R.A., Prakash J. 2007. The impact of germination and dehulling on nutrients, antinutrients, in vitro iron and calcium bioavailability and in vitro starch and protein digestibility of some legume seed. LWT, 40: 1292-1299.
• Khan S., Verma G., Sharma S. 2010. A novel Ca2+-activated protease from germinating Vigna radiata seeds andits role in storage protein mobilization. J. Plant Physiol., 167: 855-861.
• Kuo Y-H., Rozan P., Lambein F., Frias J., Vidal-Valverde C. 2004. Effects of different germination conditions on the contents of free protein and non-protein amino acids of commercial legumes. Food Chem., 86: 537-545.
• Lowry O.H. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem., 193: 265-275.
• Muntz K., Belozersky M.A., Dunaevsky Y.E., Schlereth A., Tiedemann J. 2001. Stored proteinases and the initiation of storage protein mobilization in seeds during germination and seedling growth. J. Exp. Bot., 52: 1741-1752.
• Ogiwara N., Amano T., Satoh M., Shioi Y. 2005. Leucine aminopeptidase from etiolated barley seedlings: characterization and partial purification of isoforms. Plant Sci., 168: 575-581.
• Palma J.M., Sandalio L.M., Corpas F.J., Romero-Puertas M.C., Mccarthy I., Del Río L.A. 2002. Plant proteases, protein degradation, and oxidative stress: role of peroxisomes. Plant Physiol. Biochem., 40(6-8): 521-530.
• Periago M.J., Ros G., Martínez C., Rincón F. 1996. Variations of non-protein nitrogen in six Spanish legumes according to the extraction method used. Food Res. Int., 29: 489-494.
• Randhir R., Shett y K. 2007. Elicitation of the proline-linked pentose phosphate pathway metabolites and antioxidant enzyme response by ascorbic acid in dark germinated fava bean sprouts. J. Food Biochem., 31: 485-508.
• Rodrígueza C., Frias J., Vidal-Valverde C., Hernández A. 2008. Correlations between some nitrogen fractions, lysine, histidine, tyrosine, and ornithine contents during the germination of peas, beans, and lentils. Food Chem., 108(1): 245-252.
• Rozan P., Kuo Y-H., Lambein F. 2001. Amino acids in seed and seedlings of genus Lens. Phytochem., 58: 281-289.
• Suna S-W., Lina Y-C., Wenga Y-M., Chen M-J. 2006. Efficiency improvements on ninhydrin method for amino acid quantification. J. Food Compos. Anal., 19: 112-117.
• Świeca M., Gawlik-Dziki U., Kowalczyk D., Złotek U. 2012. Impact of germination time and type of illumination on the antioxidant compounds and antioxidant capacity of Lens culinaris sprouts. Sci. Hort., 140: 87-95.
• Zehra A., Gul B., Ansari R., Khan M.A. 2012. Role of calcium in alleviating effect of salinity on germination of Phragmites karka seeds. S. Afr. J. Bot., 78: 122-128.
• Zhao J., Davis L.C., Verpoorte R. 2005. Elicitor signal transduction leading to production of plant secondary metabolites. Biotechnol. Adv., 23(4): 283-333.

Uwagi

PL

Rekord w opracowaniu

Identyfikatory

DOI

10.5601/jelem.2014.19.2.650