Functional analysis of tomato LeEIL1 in an Arabidopsis ein2 mutant

• Autorzy: Pan Y. , Chen G. , Lu C. , Chen X. , Hu Z.
• Języki publikacji: EN

Abstrakty

EN

The ETHYLENE-INSENSITIVE3 (EIN3)/EIN3- Like (EIL) EIN3/EILs, novel nuclear proteins, are located at the downstream position of the ethylene signal transduction pathway. LeEIL1, which is expressed in fruit throughout ripening, is key transcription factor in the ethylene signaling pathway in tomato. To reveal its function, the LeEIL1 gene has been transformed into and expressed in the ein2 mutant of Arabidopsis. The expression levels of the transgene in the single copy line, LeEIL1-ein2-b, were higher than those in the multiple-copy line, LeEIL1-ein2-a. The ethylene-insensitive phenotype of the ein2 mutant plants has been partially recovered by expression of LeEIL1. The florescence of LeEIL1-ein2-a and LeEIL1- ein2-b exceeded that of the ein2 mutant but was still less than that of wild type of Arabidopsis. The expression of four pathology-related genes (AtPR3, 4, AtPDF1.2 and AtGST2) has been analyzed in LeEIL1 transgenic ein2 mutant plants. The expression of AtPR3 and AtPR4, which was reduced in the ein2 mutant, was enhanced in the two transgenic Arabidopsis plants. The expression of the AtPDF1.2 gene was unaffected in the two transgenic Arabidopsis lines, the ein2 mutant and wild-type Arabidopsis plants. In addition, the expression level of AtGST2 in transgenic Arabidopsis plants was lower even than that in ein2 mutant and wild-type Arabidopsis plants.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2011
• Tom: 33
• Numer: 2
• Opis fizyczny: p.489-496,fig.,ref.

Twórcy

autor

Pan Y.

• College of Bioengineering, Chongqing University, 400044 Chongqing, People's Republic of China

autor

Chen G.

• College of Bioengineering, Chongqing University, 400044 Chongqing, People's Republic of China

autor

Lu C.

• Multidisciplinary Centre for Integrative Biology, School of Biosciences, University of Nottingham, Sutton Bonington, Leicestershire LE12 5RD, UK

autor

Chen X.

• Beijing Agro-Biotechnology Research Center, 100089 Beijing, People's Republic of China

autor

Hu Z.

• College of Bioengineering, Chongqing University, 400044 Chongqing, People's Republic of China

Bibliografia

• Alonso JM, Stepanova AN (2004) The ethylene signaling pathway. Science 306:1513–1515
• Alonso JM, Hirayama T, Roman G, Nourizadeh S, Ecker JR (1999) EIN2, a bifunctional transducer of ethylene and stress responses in Arabidopsis. Science 284:2148–2152
• Alonso JM, Stepanova AN, Solano R, Wisman E, Ferrari S, Ausubel FM, Ecker JR (2003) Five components of the ethylene-response pathway identified in a screen for weak ethylene-insensitive mutants in Arabidopsis. Proc Natl Acad Sci USA 100:2992–2997
• Binder BM, Mortimore LA, Stepanova AN, Ecker JR, Bleecker AB (2004) Short-term growth responses to ethylene in Arabidopsis seedlings are EIN3/EIL1 independent. Plant Physiol 136: 2921–2927
• Bleecker AB, Kende H (2000) Ethylene: a gaseous signal molecule in plants. Annu Rev Cell Dev Biol 16:1–18
• Chang C, Shockey JA (1999) The ethylene-response pathway: signal perception to gene regulation. Curr Opin Plant Biol 2:352–358
• Chao Q, Rothenberg M, Solano R, Roman G, Terzaghi W, Ecker JR (1997) Activation of the ethylene gas response pathway in Arabidopsis by the nuclear protein ETHYLENE-INSENSITIVE3 and related proteins. Cell 89:1133–1144
• Chen QG, Bleecker AB (1995) Analysis of ethylene signal-transduction kinetics associated with seedling-growth response and chitinase induction in wild-type and mutant arabidopsis. Plant Physiol 108:597–607
• Chen G, Alexander L, Grierson D (2004) Constitutive expression of EIL-like transcription factor partially restores ripening in the ethylene-insensitive Nr tomato mutant. J Exp Bot 55:1491–1497
• Chen YF, Etheridge N, Schaller GE (2005) Ethylene signal transduction. Ann Bot 95:901–915
• Chen G, Hu Z, Grierson D (2008) Differential regulation of tomato ethylene responsive factor LeERF3b, a putative repressor, and the activator Pti4 in ripening mutants and in response to environmental stresses. J Plant Physiol 165:662–670
• Clough SJ, Bent AF (1998) Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J 16:735–743
• Dellaporta S, Wood J, Hicks J (1983) A plant DNA minipreparation: Version II. Plant Mol Biol Report 1:19–21
• Ecker JR (1995) The ethylene signal transduction pathway in plants. Science 268:667–675
• Feys BJ, Parker JE (2000) Interplay of signaling pathways in plant disease resistance. Trends Genet 16:449–455
• Fujimoto SY, Ohta M, Usui A, Shinshi H, Ohme-Takagi M (2000) Arabidopsis ethylene-responsive element binding factors act as transcriptional activators or repressors of GCC box-mediated gene expression. Plant Cell 12:393–404
• Gu YQ, Wildermuth MC, Chakravarthy S, Loh YT, Yang C, He X, Han Y, Martin GB (2002) Tomato transcription factors pti4, pti5, and pti6 activate defense responses when expressed in Arabidopsis. Plant Cell 14:817–831
• Guo H, Ecker JR (2004) The ethylene signaling pathway: new insights. Curr Opin Plant Biol 7:40–49
• Hirsch J, Deslandes L, Feng DX, Balague C, Marco Y (2002) Delayed symptom development in ein2-1, an Arabidopsis ethyleneinsensitive mutant, in response to bacterial wilt caused by Ralstonia solanacearum. Phytopathology 92:1142–1148
• Hongxing Z, Benzhong Z, Bianyun Y, Yanling H, Daqi F, Wentao X, Yunbo L (2005) Cloning and DNA-binding properties of ethylene response factor, LeERF1 and LeERF2, in tomato. Biotechnol Lett 27:423–428
• Hua J, Chang C, Sun Q, Meyerowitz EM (1995) Ethylene insensitivity conferred by Arabidopsis ERS gene. Science 269:1712–1714
• Hua J, Sakai H, Nourizadeh S, Chen QG, Bleecker AB, Ecker JR, Meyerowitz EM (1998) EIN4 and ERS2 are members of the putative ethylene receptor gene family in Arabidopsis. Plant Cell 10:1321–1332
• Huanga S, Sawakia T, Mizunoa S, Takezawab K, Matsumurab A, Yokotsukaa M, Hirasawaa Y, Sonodaa M, Nakagawaa M, Sato T (2010) Melon EIN3-like transcription factors (CmEIL1 and CmEIL2) are positive regulators of an ethylene- and ripeninginduced 1-aminocyclopropane-1-carboxylic acid oxidase gene (CM-ACO1). Plant Sci 178:251–257
• Johnson PR, Ecker JR (1998) The ethylene gas signal transduction pathway: a molecular perspective. Annu Rev Genet 32:227–254
• Lashbrook CC, Tieman DM, Klee HJ (1998) Differential regulation of the tomato ETR gene family throughout plant development. Plant J 15:243–252
• Mao C, Wang S, Jia Q, Wu P (2006) OsEIL1, a rice homolog of the Arabidopsis EIN3 regulates the ethylene response as a positive component. Plant Mol Biol 61:141–152
• Ouaked F, Rozhon W, Lecourieux D, Hirt H (2003) A MAPK pathway mediates ethylene signaling in plants. EMBO J 22:1282–1288
• Potuschak T, Lechner E, Parmentier Y, Yanagisawa S, Grava S, Koncz C, Genschik P (2003) EIN3-dependent regulation of plant ethylene hormone signaling by two arabidopsis F box proteins: EBF1 and EBF2. Cell 115:679–689
• Qiao H, Chang KN, Yazaki J, Ecker JR (2009) Interplay between ethylene, ETP1/ETP2 F-box proteins, and degradation of EIN2 triggers ethylene responses in Arabidopsis. Genes Dev 23: 512–521
• Roman G, Lubarsky B, Kieber JJ, Rothenberg M, Ecker JR (1995) Genetic analysis of ethylene signal transduction in Arabidopsis thaliana: five novel mutant loci integrated into a stress response pathway. Genetics 139:1393–1409
• Roy S, Garges S, Adhya S (1998) Activation and repression of transcription by differential contact: two sides of a coin. J Biol Chem 273:14059–14062
• Sakai H, Hua J, Chen QG, Chang C, Medrano LJ, Bleecker AB, Meyerowitz EM (1998) ETR2 is an ETR1-like gene involved in ethylene signaling in Arabidopsis. Proc Natl Acad Sci USA 95:5812–5817
• Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor
• Schaller GE, Kieber JJ (2009) Ethylene. In: The arabidopsis book. The American Society of Plant Biologists, pp 1–18
• Seifert GJ, Barber C, Wells B, Roberts K (2004) Growth regulators and the control of nucleotide sugar flux. Plant Cell 16:723–730
• Solano R, Stepanova A, Chao Q, Ecker JR (1998) Nuclear events in ethylene signaling: a transcriptional cascade mediated by ETHYLENE-INSENSITIVE3 and ETHYLENE-RESPONSEFACTOR1. Genes Dev 12:3703–3714
• Stepanova AN, Yun J, Likhacheva AV, Alonso JM (2007) Multilevel interactions between ethylene and auxin in Arabidopsis roots. Plant Cell 19:2169–2185
• Tieman DM, Klee HJ (1999) Differential expression of two novel members of the tomato ethylene-receptor family. Plant Physiol 120:165–172
• Tieman DM, Ciardi JA, Taylor MG, Klee HJ (2001) Members of the tomato LeEIL (EIN3-like) gene family are functionally redundant and regulate ethylene responses throughout plant development. Plant J 26:47–58
• Tournier B, Sanchez-Ballesta MT, Jones B, Pesquet E, Regad F, Latche A, Pech JC, Bouzayen M (2003) New members of the tomato ERF family show specific expression pattern and diverse DNA-binding capacity to the GCC box element. FEBS Lett 550:149–154
• Wang J, Chen G, Hu Z, Chen X (2007) Cloning and characterization of the EIN2-homology gene LeEIN2 from tomato. DNA Seq 18:33–38
• Wang X, Kong H, Ma H (2009) F-box proteins regulate ethylene signaling and more. Genes Dev 23:391–396
• Wilkinson JQ, Lanahan MB, Yen HC, Giovannoni JJ, Klee HJ (1995) An ethylene-inducible component of signal transduction encoded by never-ripe. Science 270:1807–1809
• Wu K, Tian L, Hollingworth J, Brown DC, Miki B (2002) Functional analysis of tomato Pti4 in Arabidopsis. Plant Physiol 128:30–37
• Yang SF, Hoffman NE (1984) Ethylene biosynthesis and its regulation in higher plants. Ann Rev Plant Physiol 35:155–189
• Yang Y, Wu Y, Pirrello J, Regad F, Bouzayen M, Deng W, Li Z (2010) Silencing Sl-EBF1 and Sl-EBF2 expression causes constitutive ethylene response phenotype, accelerated plant senescence, and fruit ripening in tomato. J Exp Bot 61(3): 697–708
• Yokotani N, Tamura S, Nakano R, Inaba A, Kubo Y (2003) Characterization of a novel tomato EIN3-like gene (LeEIL4). J Exp Bot 54:2775–2776
• Yoo SD, Cho YH, Tena G, Xiong Y, Sheen J (2008) Dual control of nuclear EIN3 by bifurcate MAPK cascades in C2H4 signalling. Nature 451:789–795

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