Clinical, imaging and genetic analysis of double bilateral radix entomolaris

• Autorzy: Rodríguez-Niklitschek C.A. , Oporto V. G.H. , Garay I. , Salazar L.A.
• Języki publikacji: EN

Abstrakty

EN

Background: Anatomy describes that first mandibular molars have two roots: 1 mesial, with 2 root canals, and 1 distal, with 1 root canal. The presence of three roots in these teeth is uncommon. Root anatomical variations have an impact, especially in endodontic, where the highest rates of nonsurgical treatment failures are due to the inability to identify and access roots and/or accessory canals. The aim of this research is to report a case of double three-rooted mandibular first molar through clinical, imaging and genetic analysis. Materials and methods: Using a panoramic radiography, the presence of three roots in teeth 36 and 46 was diagnosed in a female patient. Additionally, it was indicated a cone beam computed tomography. Moreover, leukocyte genomic DNA was obtained from a blood sample of the patient to determine her ethnicity through analysis of mitochondrial DNA haplogroups using polymerase chain reaction-length restriction fragment polymorphism (PCR-RFLP). Results: Both molars had three roots, 1 mesial (M), 1 distolingual (DL), also known as radix entomolaris (RE), and a distovestibular (DV). For both teeth, M root had 2 canals, and DV and DL roots presented just 1 canal. Mitochondrial DNA analysis determined presence of haplogroup C, corresponding to Amerindian ethnicity. Conclusions: The presence of RE is uncommon. This case report contributes to describe this rare anatomical variation. To our knowledge, this is the first molecular-genetic study applied to dental anatomy and gives basis to develop future research in the area. (Folia Morphol 2015; 74, 1: 127–132)

• Wydawca: -
• Czasopismo: Folia Morphologica
• Rocznik: 2015
• Tom: 74
• Numer: 1
• Opis fizyczny: p.127-133,fig.,ref.

Twórcy

autor

Rodríguez-Niklitschek C.A.

• Departamento de Odontología Integral Adultos, Facultad de Odontología, Universidad de La Frontera, Manuel Montt 112, Temuco, Chile

autor

Oporto V. G.H.

• Centro de Biología Moleculary Farmacogenetica, Facultad de Medicina, Universidad de La Frontera, Temuco, Chile
• Departamento de Odontología Integral Adultos, Facultad de Odontología, Universidad de La Frontera, Temuco, Chile

autor

Garay I.

• Departamento de Odontología Integral Adultos, Facultad de Odontología, Universidad de La Frontera, Temuco, Chile

autor

Salazar L.A.

• Centro de Biología Moleculary Farmacogenetica, Facultad de Medicina, Universidad de La Frontera, Temuco, Chile

Bibliografia

• 1. Abella F, Mercadé M, Duran-Sindreu F, Roig M (2011) Managing severe curvature of radix entomolaris: three-dimensional analysis with cone beam computed tomography. Int Endod J, 44: 876–885.
• 2. Abella F, Patel S, Durán-Sindreu F, Mercadé M, Roig M (2012) Mandibular first molars with disto-lingual roots: review and clinical management. Int Endod J, 45: 963–978.
• 3. Al-Qudah A, Awawdeh L (2009) Root and canal morphology of mandibular first and second molar teeth in a Jordanian population. Int Endod J, 42: 775–784.
• 4. Calberson FL, De Moor RJ, Deroose CA (2007) The radix entomolaris and paramolaris: clinical approach in endodontics. J Endod, 33: 58–63.
• 5. Chandra SS, Chandra S, Shankar P, Indira R (2011) Prevalence of radix entomolaris in mandibular permanent first molars: a study in a South Indian population. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 112: 77–82.
• 6. De Moor RJ, Deroose CA, Calberson FL (2004) The radix entomolaris in mandibular first molars: an endodontic challenge. Int Endod J, 37: 789–799.
• 7. Guzman N, Lanas F, Salazar L (2010) Influence of Amerindian mitochondrial DNA haplogroups on thrombosis susceptibility and frequency of four genetic prothrombotic variants in southern Chilean subjects. Clin Chim Act, 411: 444–447.
• 8. Guzman N, Salazar L (2010) Frecuency of prothrombotic risk factors in patients with deep venous thrombosis and controls: Their implications for thrombophilia screening in Chilean subjects. Genet Test Mol Biomark, 14: 1–4.
• 9. Huang C, Chang Y, Chuang M, Lai T, Lai J, Lee B, Lin C (2010) Evaluation of root and canal systems of mandibular first molars in Taiwanese individuals using cone-beam computed tomography. J Formos Med Assoc, 109: 303–308.
• 10. Huang R, Cheng W, Chen C, Lin C, Lai T, Shen E, Chiang C, Chiu H, Fu E (2010) Three-dimensional analysis of the root morphology of mandibular first molars with distolingual roots. Int Endod J, 43: 478–484.
• 11. Kupczik K, Hublin J (2010) Mandibular molar root morphology in Neanderthals and Late Pleistocene and recent Homo sapiens. J Hum Evol, 59: 525–41.
• 12. Lagos J, Lemus J, Sierra F, Mella R, Fuentes F, Ocares G, Rosales A, Salazar L, Durán D, Guzmán N (2010) Asociación de niveles de lípidos y haplogrupos Amerindios de DNA mitocondrial en individuos chilenos hipercolesterolémicos tratados con Atorvastatina. Rev Chil Cardiol, 29: 208–213.
• 13. Moraga ML, Rocco P, Miquel JF, Nervi F, Llop E, Chakraborty R, Rothhammer F, Carvallo P (2000) Mitochondrial DNA polymorphisms in Chilean aboriginal populations: implications for the peopling of the southern cone of the continent. Am J Phys Anthropol, 113: 19–29.
• 14. Oporto VGH, Fuentes FRE, Soto PCC (2010) Tooth root and pulp canal anatomical variations. Int J Morphol, 28: 945–950.
• 15. Oporto VGH, Saavedra R, Fuentes R, Soto PCC (2013) Double root anatomical variations in a single patient: endodontic treatment and rehabilitation of a three-rooted first premolar. Case report. Int J Morphol, 31: 45–49.
• 16. Rocco P, Morales C, Moraga M, Miquel JF, Nervi F, Llop E, Carvallo P, Rothhammer F (2002) Genetic composition of the Chilean population. Analysis of mitochondrial DNA polymorphisms. Rev Méd Chile, 130: 125–131.
• 17. Soares J, Leonardo R (2003) Root canal treatment of threerooted maxillary first and second premolars: a case report. Int Endod J, 36: 705–710.
• 18. Wang Q, Yu G, Zhou XD, Peters OA, Zheng QH, Huang DM (2011) Evaluation of x-ray projection angulation for successful radix entomolaris diagnosis in mandibular first molars in vitro. J Endod, 37: 1063–1068.
• 19. Zhang R, Wang H, Tian Y, Yu X, Hu T, Dummer P (2011) Use of cone-beam computed tomography to evaluate root canal and canal morphology of mandibular molars in Chinese individuals. Int Endod J, 44: 990–999.

Identyfikatory

DOI

10.5603/FM.2015.0018