Prevalence and characterisation of Salmonella in slaughtered cattle and beef in Poland

• Autorzy: Wieczorek K. , Osek J.
• Języki publikacji: EN

Abstrakty

EN

A total of 1229 samples, including 406 bovine hides and 406 corresponding carcasses at the slaughter level, as well as 417 beef meat from local supermarkets, were tested for the presence of Salmonella sp. Eighteen ( 1.5%) samples were positive for the target microorganism, and the highest prevalence (2 2%) was found in meat, followed by carcasses (1.2%) and hides (1.0%). Among the isolated strains. Salmonella enterica serotypes Enteritidis (six isolates) and Schleissheim (six strains), followed by Dublin (four contaminated samples) were the most predominant. The antimicrobial resistance analysis against nine antimicrobials with the MIC technique revealed that most isolates were sensitive to all antibacterial agents. However, one S. Typhimurium of carcass origin was multidrug resistant, and displayed the resistance to four antimicrobials, i.e. ampicillin, streptomycin, tetracycline, and sulphametoxazole. Furthermore, one S. Enteritidis (from carcasses), two S. Dublin (of beef origin), and one S. London (from meat) strains were resistant to sulphametoxazole. The restriction enzyme analysis with XbaI resulted in eight different PFGE types. The obtained results suggest that cattle may be an underestimated source of pathogenic Salmonella for consumers.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2013
• Tom: 57
• Numer: 4
• Opis fizyczny: p.607-611,fig.,ref.

Twórcy

autor

Wieczorek K.

• Department of Hygiene of Food of Animal Origin, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Osek J.

• Department of Hygiene of Food of Animal Origin, National Veterinary Research Institute, 24-100 Pulawy, Poland

Bibliografia

• 1. Alcaine S.D., Warnick L.D., Wiedmann M.: Antimicrobial resistance in nontyphoidal Salmonella. J Food Prot 2007, 70, 780-790.
• 2. Bacon R.T., Sofos J.N., Belk K.E., Smith G.C. Prevalence and antibiotic susceptibility of Salmonella isolated from beef animal hides and carcasses. J Food Prot 2002, 64, 284 -290.
• 3. Barkocy-Gallagher G.A., Arthur T.M., Rivera Betancourt M., Nou X., Shackelford S.D., Wheeler T.L., Koohmaraie M.: Seasonal prevalence of shiga toxin- producing Escherichia coli, including O157:H7 and non-O157 serotypes, and Salmonella in commercial beef processing plants. J Food Prot 2003, 66, 1978- 1986.
• 4. Bingol E.B., Dumen E., Kahraman T., Akhan M., Issa G., Ergun O.: Prevalence of Salmonella spp., Listeria monocytogenes and Escherichia coli O157 in meat and meat products consumed in Istanbul. Med Weter 2013, 69, 488-491.
• 5. Bosilevac J.M., Guerini M.N., Kalchayanand N., Koohmaraie M.: Prevalence and characterization of salmonellae in commercial ground beef in the United States. Appl Environ Microbiol 2009, 75, 1809-1900.
• 6. Brichta Harhay D.M., Arthur T.M., Bosilevac J.M., Kalchayanand N., Shackelford S.D., Wheeler T.L., Koohmaraie M.: Deversity of multidrug-resistant Salmonella enterica strains associated with cattle at harvest m the United States. Appl Environ Microbiol 2011, 77, 1783-1796.
• 7. Brichta-Harhay D.M., Guerini M.N., Arthur T.M., Bosilevac J.M., Kalchayanand N., Shackelford S.D., Wheeler T.L., Koohmaraie M.: Salmonella and Escherichia coli O157:H7 contamination on hides and carcasses of cull cattle presented for slaughter in the United States: an evaluation of prevalence and bacterial loads by immunomagnetic separation and direct plating methods. Appl Environ Microbiol 2008, 74, 6289-6297.
• 8. D'Aoust J.Y.: Pathogenicity of foodborne Salmonella. Int J Food Microbiol 1991, 12, 17-40.
• 9. European Food Safety Authority, European Centre for Disease Prevention and Control: the European Union summary report on trend and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2011. EFSA J 2013, 11, 3129.
• 10. European Food Safety Authority, European Centre for Disease Prevention and Control: the European Union summary report on antimicrobial resistance in zoonotic and indicator bacteria from humans, animals and food in 2011. EFSA J 2013,11, 3196.
• 11. Ghafir Y., China B., Korsak N., Dierick K., Collard J.M., Godard C., de Zutter L., Daube G.: Belgian surveillance plans to assess changes in Salmonella prevalence in meat at difierent production stages. J Food Prot 2005, 68, 2269-2277.
• 12. Cirimont P.A.D., Weill F.-X.: Antigenic formulas of Salmonella serovars, 9th Edition WHO Collaborating Centre for Research on Salmonella. Institute Pasteur, Paris, 2007.
• 13. Madden R.H., Espie W.E., Moran L., McBride J., Scates P.: Occurrence of Escherichia coli O157:H7, Listeria monocytogenes. Salmonella and Campylobacter spp. on beef carcasses in Northern Ireland. Meat Sci 2001, 58, 343-346.
• 14. McEvoy J.M., Doherty A.M., Sheridan J.J., Blair I.S., McDowell D.A.: The prevalence of Salmonella spp. in bovine faecal, rumen and carcass samples at a commercial abattoir. J Appl Microbiol 2003, 94, 693-700.
• 15. Narvaez-Bravo C., Miller M.F., Jackson T., Jackson S., Rodas -Gonzalez A., Pond K., Echeverry A., Brashears M.M.: Salmonella and Escherichia coli O157:H7 prevalence in cattle and on carcasses in a vertically integrated feedlot and harvest plant in Mexico. J Food Prot 2013, 76, 786-795.
• 16. Narvaez-Bravo C., Rodas-Gonzalez A., Fuenmayor Y., Flores -Rondon C., Carruyo G., Moreno M., Perozo-Mena A., Hoet A.E.: Salmonella on feces, hides and carcasses in beef slaughter facilities in Venezuela. Intern J Food Microbiol 2013, 166, 226-230.
• 17. Paszkiewicz W., Pyz-Łukasik R.: Bacterial contamination of carcass surfaces in relation to the order of slaughtering cattle. Med Weter 2010, 66, 51-53.
• 18. Reid C.A., Small A., Avery S.M., Buncic S.: Presence of food borne pathogens on cattle hides. Food Control 2002, 13, 411-415.
• 19. Rhoades J.R., Duffy G., Koutsoumanis K.: Prevalence and concentration of verocytotoxigenic Escherichia coli. Salmonella enterica and Listeria monocytogenes in the beef production chain: a review. Food Microbiol 2009, 26, 357-376.
• 20. Sofos J.N., Kochevar S.L., Reagan J.O., Smith G.C.: Incidence of Salmonella on beef carcasses relating to the US meat and poultry inspection regulations. J Food Prot 1999, 62. 467-473.
• 21. Stevens A., Kerouanton A., Marault M., Millemann Y., Brisabois A., Cavin J.-F., Dufour B.: Epidemiological analysis of Salmonella enterica from beef sampled in the slaughterhouse and retailers in Dakar (Senegal) using pulsed-field gel electrophoresis and antibiotic susceptibility testing. Intern J Food Microbiol 2008, 123, 191-197.
• 22. http://wwv.cdc.gov/pulsenet/ pathogens/salmonella.htm.

Identyfikatory

DOI

10.2478/bvip-2013-0103