Determination of thiamphenicol and florphenicol in the chicken kidneys and liver by liquid chromatography-mass spectrometry

• Autorzy: Sniegocki T. , Posyniak A. , Zmudzki J.
• Języki publikacji: EN

Abstrakty

EN

An effective liquid chromatographic method with tandem mass spectrometric (LC-MS/MS) detection and identification was developed for the determination of thiamphenicol and florphenicol in the chicken liver and kidneys. After a preliminary dissolution in ethyl acetate and a homogenisation step, the obtained extract was evaporated to dryness and dissolved in water. The solution was clean up on an octadecyl (C18) solid phase extraction (SPE) cartridge. LC-MS/MS, electrospray ionisation in the negative ion mode was used for determination of the compounds followed by separation on the octadecyl column. The whole procedure was validated according to the requiments of Commission Decision 2002/657/EC and the samples were spiked with thiamphenicol and florphenicol at the levels of 50-75-100-500 µg kg⁻¹. At these levels, recovery ranged between 95.0% and 103.0%, and the within-laboratory reproducibility was lower than 5.03%. The limit of decision (CCα) for thiamphenicol and florphenicol in the kidneys were established at the level of 55.3 µg kg⁻¹ and 54.4 µg kg⁻¹, respectively, and in the liver at the level of 55.3 µg kg⁻¹ and 54.7 µg kg⁻¹. The results of the validation demonstrated that this method meets criteria for determination and confirmation of thiamphenicol and florfenicol in biological material.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2011
• Tom: 55
• Numer: 4
• Opis fizyczny: p.749-753,fig.,ref.

Twórcy

autor

Sniegocki T.

• Department of Pharmacology and Toxicology, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Posyniak A.

autor

Zmudzki J.

Bibliografia

• 1. Anadon A., Martinez M. A., Martinez M., Rios A., Caballero V., Ares I., Martinez-Larranaga M. R.: Plasma and tissue depletion of florfenicol and florfenicol-amine in chickens. J Agric Food Chem 2008, 56, 11049-11056.
• 2. Commission Decision of 12 August 2002 2002/657/EC.
• 3. Festing M.F., Diamanti P., Turton J.A.: Strain differences in haematological response to chloramphenicol succinate in mice: implications for toxicological research. Food Chem Toxicol 2001, 39, 375-383.
• 4. Hormazabal V., Steffenak I., Yndestad M.: Simultaneous determination of residues of florfenicol and the metabolite florfenicol amine in fish tissues by high performance liquid chromatography. J Chromatogr 1993, 616, 161-165.
• 5. Kijak P.J.: Confirmation of chloramphenicol residues in bovine milk by gas chromatography/mass spectrometry. J AOAC Int 1994, 77, 34-40.
• 6. Li J., Ding S., Zhang S., Li C., Li X., Liu Z., Liu J., Shen J.: Residue depletion of florfenicol and its metabolite florfenicol amine in swine tissues after intramuscular administration. J Agric Food Chem 2006, 54, 9614-9619.
• 7. Li P., Qiu Y., Cai H., Kong Y., Tang Y., Wang D., Xie M.: Simultaneous determination of chloramphenicol, thiamphenicol, and florfenicol residues in animal tissues by gas chromatography/mass spectrometry. Ch J Chromatogr 2006, 24, 14—18.
• 8. Luo P., Jiang H., Feng Z.C., He F., Shen J.: Simultaneous determination of florfenicol and its metabolite florfenicol amine in swine muscle tissue by a heterologous enzyme-linked immunosorbent assay. J AOAC Int 2009, 92, 981-988.
• 9. Liu W.L., Lee R.J., Lee M.R.: Supercritical fluid extraction in situ derivatization for simultaneous determination of chloramphenicol, florfenicol and thiamphenicol in shrimp. Food Chem 2010, 121, 797-802.
• 10. Nagata T., Oka H.: Detection of residual chloramphenicol, florfenicol, and thiamphenicol in yellowtail fish muscles by capillary gas chromatography-mass spectrometry. J Agric Food Chem 1996, 44, 1280-1284.
• 11. Nagata T., Saeki M.: Simultaneous determination of thiamphenicol, florfenicol, and choramphenicol residues in muscles of animals and cultured fish by liquid chromatography. J Liq Chromatogr 1992, 15, 2045-2056.
• 12. Pfenning A.P., Roybal J.E., Rupp H.S., Turnipseed S.B., Gonzales S.A., Hurlbut J.A.: Simultaneous determination of residues of chloramphenicol, florfenicol, florfenicol amine, and thiamphenicol in shrimp tissue by gas chromatography with electron capture detection. J AOAC Int 2000, 83, 26-30.
• 13. Pfenning A.P., Madson M.R., Roybal J.E., Turnipseed S.B., Gonzales S.A., Hurlbut J.A., Salmon G.D.: Simultaneous determination of chloramphenicol, florfenicol, and thiamphenicol residues in milk by gas chromatography with electron capture detection. J AOAC Int 1998, 81, 714-720.
• 14. van de Riet J.M., Potter R.A., Christie-Fougere M., Burns B.G.P.: Simultaneous determination of residues of chloramphenicol, thiamphenicol, florfenicol, and florfenicol amine in farmed aquatic species by liquid chromatography/mass spectrometry. J AOAC Int 2003, 86, 510-514.
• 15. Shena J., Xia X., Jianga H., Li C., Li J., Li X., Dinga S.: Determination of chloramphenicol, thiamphenicol, florfenicol, and florfenicol amine in poultry and porcine muscle and liver by gas chromatography-negative chemical ionization mass spectrometn. J Chromatogr В 2009, 877, 1523-1529.
• 16. Switała M., Hrynyk R., Smutkiewicz A., Jaworski K., Pawlowski P., Okoniewski P., Grabowski T., Dębowy J.: Pharmacokinetics of florfenicol, thiamphenicol, and chloramphenicol in turkeys. J Vet Pharmacol Ther 2007, 30, 145-150.
• 17. Śniegocki T., Posyniak A., Żmudzki J.: Validation of the gas chromatography-mass spectrometry method for the determination of chloramphenicol residues in milk. Bull Vet Inst Pulawy, 2006, 50, 353-357.
• 18. Śniegocki T., Posyniak A., Żmudzki J.: Determination of chloramphenicol residues in milk by gas and liquid chromatography mass spectrometry methods. Bull Vet Inst Pulawy, 2007, 51, 59-64.
• 19. Wrzesinski C.L., Crouch L., Endris S.: Determination of florfenicol amine in channel catfish muscle by liquid chromatography. J AOAC Int 2003, 86, 515-520.
• 20. Wrzesinski C.L., Crouch L., Gaunt P., Holifield D., Bertrand N., Endris R.: Florfenicol residue depletion in channel catfish, Ictalurus punctatus (Rafinesque). Aquaculture 2006, 253, 309-316.
• 21. Wu J.E., Chang C., Ding W.P., He D.P.: Determination of florfenicol amine residues in animal edible tissues by an indirect competitive ELISA. J Agric Food Chem, 2008, 56, 8261-8267.
• 22. Zhang S.X., Liu Z., Guo X., Cheng L., Wang Z., Shen J.: Simultaneous determination and confirmation of chloramphenicol, thiamphenicol, florfenicol and florfenicol amine in chicken muscle by liquid chromatography-tandem mass spectrometry. J Chromatogr В 2008, 875, 399-404.
• 23. Zhang S.X., Sun F.Y., Li J.C., Cheng L.L., Shen J.Z.: Simultaneous determination of florfenicol and florfenicol amine in fish, shrimp, and swine muscle by gas chromatography with a microcell electron capture detector. J AOAC Int 2006, 89, 1437.

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