The porcine brucellosis - evidence of the role of Yersinia enterocolitica O:9 in occurrence of false positive serological reactions

• Autorzy: Weiner M. , Szulowski K. , Iwaniak W.
• Języki publikacji: EN

Abstrakty

EN

Forty four pigs with typical characteristics for false positive serological reactions (FPSR) were examined for the presence of Yersinia enterocolitica O:9. The positive reactions were observed in rose bengal test (RBT, N=23 sera), serum agglutination test (SAT, N=16), complement fixation test (CFT, N=9), indirect ELISA (i-ELISA, N=11) in first, and in RBT (N=14), SAT (N=8), CFT (N=7) and i-ELISA (N=18) in second examination, respectively. In bacteriological examination Y. enterocolitica was confirmed in 12 cases. Six of these isolates were identified with PCR as Y. enterocolitica O:9.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2013
• Tom: 16
• Numer: 1
• Opis fizyczny: p.129-130,ref.

Twórcy

autor

Weiner M.

• Department of Microbiology, National Veterinary Research Institute, Al.Partyzantow 57, 24-100 Pulawy, Poland

autor

Szulowski K.

• Department of Microbiology, National Veterinary Research Institute, Al.Partyzantow 57, 24-100 Pulawy, Poland

autor

Iwaniak W.

• Department of Microbiology, National Veterinary Research Institute, Al.Partyzantow 57, 24-100 Pulawy, Poland

Bibliografia

• ISO 10273:2003. Microbiology of food and animal feeding stuffs – Horizontal method for the detection of presumptive pathogenic Yersinia enterocolitica.
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• OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals (2012) 7th ed., OIE, Paris.
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• Wannet WJ, Reessink M, Brunings HA, Maas HM (2001) Detection of pathogenic Yersinia enterocolitica by a rapid and sensitive duplex PCR assay. J Clin Microbiol 39: 4483-4486.
• Weiner M, Iwaniak W, Zlotnicka J, Szulowski K (2010) Diagnosis of bovine brucellosis using traditional serological techniques and fluorescence polarisation assay. Bull Vet Inst Pulawy 54: 485-488.

Uwagi

PL

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