Wykrywanie krążącego antygenu Toxoplasma gondii metodą dot-Elisa

• Autorzy: Bitkowska E. , Dzbenski T.H.

Warianty tytułu

EN

Detection of circulating antigens of Toxoplasma gondii by a dot-Elisa technique

• Języki publikacji: PL

Abstrakty

EN

Dot-immunobinding technique was used to detect circulating Toxoplasma antigens in the sera of men and animals. Serum samples were collected from mice and rabbits infected experimentally with parasites of the RH strain of T. gondii while human sera were obtained from persons suspected of having active toxoplasmosis. Circulating Toxoplasma antigens were detected in the samples of mice sera that had been collected since the 2-nd day of iniection and in the sera of rabbits during 2 and 3 week of iniection. From amongst 146 human sera, 35 (24%) gave positive reaction in the test while 14 (9,5%) produced doubtful results. The experiments proved the method to be sensitive, reproducible and easy to perform. It obviates the need of special equipment while most of the necessary reagents are commercially available. High percentage of doubtful results is the main drawback of the method.

Słowa kluczowe

PL

parazytologia; pasozyty; Toxoplasma gondii; antygeny; metody wykrywania; metoda dot-Elisa

• Wydawca: -
• Czasopismo: Wiadomości Parazytologiczne
• Rocznik: 1989
• Tom: 35
• Numer: 4
• Opis fizyczny: s.283-288,rys.,bibliogr.

Twórcy

autor

Bitkowska E.

• Zakład Parazytologii Lekarskiej, Państwowy Zakład Higieny w Warszawie, ul. Chocimska 24 00-791 Warszawa

autor

Dzbenski T.H.

• Zakład Parazytologii Lekarskiej, Państwowy Zakład Higieny w Warszawie, ul. Chocimska 24 00-791 Warszawa

Bibliografia

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• 2. Brooks, R. G., Sharma, S. D., Remington, J. S.: Detection of Toxoplasma gondii antigens by dot - immunobinding technique. - J. Clin. Microbiol., 21, 113-116, 1985.
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• 4. Kopaczowa, G., Dzbeński, T. H.: Wykrywanie krążącego antygenu Toxoplasma gondii. Mat. III Konferencji nauk.-szkol. nt. toksoplazmozy, Poznań 1983, 40-41, 1983.
• 5. Lowry, O., Rosebrough, N., Farr, A., Randall, R.: Protein measurement with Folin phenol reagent. - J. Biol. Chem., 193, 265-275, 1951.
• 6. Raizman, R. E., Neva, F. A.: Detection of circulating antigen in acute experimental infections with Toxoplasma gondii. - J. Infect. Dis., 132 44-48, 1975.
• 7. Turunen, H. J.: Detection of soluble antigens of Toxoplasma gondii by a four-layer modification of an enzyme immunoassay. - J. Clin. Microbiol., 17, 768-773, 1983.