First isolation of the H1N2 swine influenza virus in Polish pig farm

• Autorzy: Markowska-Daniel I. , Wierzchoslawski K. , Urbaniak K. , Kowalczyk A. , Pejsak Z.
• Języki publikacji: EN

Abstrakty

EN

The paper describes an outbreak of swine influenza, which was the first H1N2 outbreak confirmed in pig farm in Poland. The outbreak occurred in October 2011 in the farrows to finish farm, first in the fattening units, and subsequently in the reproduction and weaning sectors. Samples of the lungs taken from dead gilts were tested by the use of real time and multiplex PCR, sequencing, and virus isolation methods. The amplification of the genetic material extracted from the lungs confirmed the presence of the M1 gene sequence of type A influenza virus. Using multiplex PCR, the bands of 241 bp, typical for HA1 human-like type, and of 791 bp, typical for NA2, were demonstrated. The new isolates of swine influenza virus, named A/swine/Poland/15817/2011, were obtained in embryonated SPF chicken eggs and MDCK cells. Phylogenese analysis of the HA and NA genes of the isolate revealed that the virus is of human swine linkage closely related to European human-like viruses. On the basis of such results, it can be stated that A/Swine/Poland/15817/2011 is a new swine influenza virus, representing the H1N2 subtype strain, which emerged in Poland.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2013
• Tom: 57
• Numer: 1
• Opis fizyczny: p.9-14,fig.,ref.

Twórcy

autor

Markowska-Daniel I.

• Department of Swine Diseases, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Wierzchoslawski K.

• Veterinary Clinic Agrobiovet, 62-200 Gniezno, Poland

autor

Urbaniak K.

• Department of Swine Diseases, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Kowalczyk A.

• Department of Swine Diseases, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Pejsak Z.

• Department of Swine Diseases, National Veterinary Research Institute, 24-100 Pulawy, Poland

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Identyfikatory

DOI

10.2478/bvip-2013-0002