Comparison of flunixin meglumine and meloxicam influence on postoperative and oxidative stress in ovariohysterectomized bitches

• Autorzy: Yilmaz O. , Korkmaz M. , Jaroszewski J. J. , Yazici E. , Ulutas E. , Saritas Z. K.
• Języki publikacji: EN

Abstrakty

EN

The aim of this study was to compare the effect of flunixin meglumine (FM) and meloxicam (M) on postoperative and oxidative stress in ovariohysterectomized bitches. Twenty four bitches were divided into three groups (n=8 in each) and treated during premedication as follows: FM (2.2 mg/kg, iv, Fluvil, Vilsan, Turkey), M (0.2 mg/kg, sc, Maxicam, Sanovel, Turkey) or 0.9% saline (1 ml, iv, IE, Turkey) - control (C) group. The concentrations of serum cortisol, nitric oxide (NO), malondialdehyde (MDA), antioxidant potential (AOP) and glutation (GSH) were measured in blood samples collected during incision (0 h), closure of incision line (0.5 h) and 1, 2.5, 12 and 24 hours after incision. It was observed that cortisol level was higher at 0.5, 1 and 2.5 h in group C (p < 0.05), 0.5 h in group FM (p < 0.001), and 1 and 2.5 h in group M (p < 0.01), as compared to that determine at 0 h. Group C showed higher cortisol level during 0.5 h (p < 0.05) than that found in the other groups. Group FM displayed lower levels during 1 h (p < 0.01) and 2.5 h (p < 0.05) as compared to those observed in other groups. Concentrations of MDA, AOP and GSH between all the groups did not show any significant differences. MDA level was higher at 0.5 and 1 h in groupM (p < 0.05) than that found in group C and it was the lowest at 2.5 h in group C (p < 0.05). AOP was higher at 2.5 h in group FM and M (p < 0.05) than that observed in group C, and at 12 and 24 h in group M than that found in group C and FM. GSH did not show any significant differences between the groups. NO level in group FM after 12 h was higher (p < 0.05) than that at 0.5, 1 and 24 h. Moreover, NO level was lower at 0.5 (p < 0.01), 1 (p < 0.05) and 24 h (p < 0.05) in group FM than that observed in group C and M. In conclusion, flunixin meglumine decreases cortisol and NO levels more efficiently than meloxicam. Therefore, it is suggested that postoperative stress following ovariohysterectomy may be prevented by flunixin meglumine in bitches.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2014
• Tom: 17
• Numer: 3
• Opis fizyczny: p.493-499,fig.,ref.

Twórcy

autor

Yilmaz O.

• Department of Obstetrics and Gynaecology, Faculty of Veterinary, Afyon Kocatepe University, 03200, Afyonkarahisar, Turkey

autor

Korkmaz M.

• Department of Surgery, Faculty of Veterinary, Afyon Kocatepe University, 03200, Afyonkarahisar, Turkey

autor

Jaroszewski J. J.

• Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-719 Olsztyn, Poland

autor

Yazici E.

• Department of Obstetrics and Gynaecology, Faculty of Veterinary, Afyon Kocatepe University, 03200, Afyonkarahisar, Turkey

autor

Ulutas E.

• Department of Physiology, Faculty of Veterinary, Afyon Kocatepe University, 03200, Afyonkarahisar, Turkey

autor

Saritas Z. K.

• Department of Surgery, Faculty of Veterinary, Afyon Kocatepe University, 03200, Afyonkarahisar, Turkey

Bibliografia

• Alva N, Palomeque J, Carbonell T (2006) Nitric oxide induced by ketamine/xylazine anesthesia maintains hepatic blood flow during hypothermia. Nitric Oxide 15: 64-69.
• Baines M, Shenkin A (2002) Lack of effectiveness of short-term intravenous micronutrient nutrition in restoring plasma antioxidant status after surgery. Clin Nutr 21: 145-150.
• Benson GJ, Grubb TL, Neff-Davis C, Olson WA, Thurmon JC, Lindner DL, Tranquilli WJ, Vanio O (2000) Perioperative stress response in the dog: effect of pre-emptive administration of medetomidine. Vet Surg 29: 85-91.
• Beutler E (1975) Glutathione in red blood cell metabolism. In: Beutler E (ed) A manual of biochemical methods. Grune and Stratton, New York, pp 112-114.
• Brideau C, Van Staden C, Chan CC (2001) In vitro effects of cyclooxygenase inhibitors in whole blood of horses, dogs, and cats. Am J Vet Res 62: 1755-1760.
• Bugajski J, Gadek-Michalska A, Bugajski AJ (2004) Nitric oxide and prostaglandin systems in the stimulation of hypothalamic- pituitary-adrenal axis by neurotransmitters and neurohormones. J Physiol Pharmacol 55: 679-703.
• Chen L, Salafranca MN, Mehta JL (1997) Cyclooxygenase inhibition decreases nitric oxide synthase activity in human platelets. Am J Physiol 273: H1854-H1859.
• Church DB, Nicholson AI, Ilkiw JE, Emslie DR (1994) Effect of non-adrenal illness, anaesthesia and surgery on plasma cortisol concentrations in dogs. Res Vet Sci 56: 129-131.
• Cymeryng CB, Dada LA, Podestá EJ (1998) Effect of nitric oxide on rat adrenal zona fasciculata steroidogenesis. J Endocrinol 158: 197-203.
• Cymeryng CB, Lotito SP, Colonna C, Finkielstein C, Pomeraniec Y, Grión N, Gadda L, Maloberti P, Podestá EJ (2002) Expression of nitric oxide synthases in rat adrenal zona fasciculata cells. Endocrinology 143: 1235-1242.
• Dahle LK, Hill EG, Holman RT (1962) The thiobarbituric acid reaction and the autooxidations of polyunsaturated fatty acid methyl esters. Arch Biochem Biophys 98: 253-261.
• Desborough JP (2000) The stress response to trauma and surgery. Br J Anaesth 85: 109-117.
• Devitt CM, Cox RE, Hailey JJ (2005) Duration, complications, stress and pain of open ovariohysterectomy versus a simple method of laparoscopic-assisted ovariohysterectomy in dogs. J Am Vet Med Assoc 227: 921-927.
• Distel M, Mueller C, Bluhmki E, Fries J (1996) Safety of meloxicam: a global analysis of clinical trials. Br J Rheumatol 35 (Suppl 1): 68-77.
• Dow SW, Rosychuk RA, McChesney AE, Curtis CR (1990) Effects of flunixin and flunixin plus prednisone on the gastrointestinal tract of dogs. Am J Vet Res 51: 1131-1138.
• Durak I, Karabacak HI, Buyukkocak S, Cimen MY, Kacmaz M, Omeroglu E, Ozturk HS (1998) Impaired antioxidant defense system in the kidney tissues from rabbits treated with cyclosporine. Protective effects of vitamins E and C. Nephron 78: 207-211.
• Ehrhart-Bornstein M, Bornstein SR (2008) Cross-talk between adrenal medulla and adrenal cortex in stress. Ann NY Acad Sci 1148: 112-117.
• Engelhardt G (1996) Pharmacology of meloxicam, a new non-steroidal anti-inflammatory drug with an improved safety profile through preferential inhibition of COX-2. Br J Rheumatol 35 (Suppl 1): 4-12.
• Firth AM, Haldane SL (1999) Development of a scale to evaluate postoperative pain in dogs. J Am Vet Med Assoc 214: 651-659.
• Fox SM, Mellor DJ, Firth EC, Hodge H, Lawoko CR (1994) Changes in plasma cortisol concentrations before, during and after analgesia, anaesthesia and anaesthesia plus ovariohysterectomy in bitches. Res Vet Sci 57: 110-118.
• Förstermann U, Boissel JP, Kleinert H (1998) Expressional control of thé constitutive’ isoforms of nitric oxide synthase (NOS I and NOS III). FASEB J 12: 773-790.
• Förstermann U, Schmidt HH, Pollock JS, Sheng H, Mitchell JA, Warner TD, Nakane M, Murad F (1991) Isoforms of nitric oxide synthase. Characterization and purification from different cell types. Biochem Pharmacol 42: 1849-1857.
• Gadek-Michalska A, Spyrka J, Bugajski J (2005) Psychosocial stress affects the involvement of prostaglandins and nitric oxide in the lipopolysaccharide-induced hypothalamic- pituitary-adrenal response. J Physiol Pharmacol 56: 287-298.
• Gunay A, Gunes N, Gunay U (2011) Effect of ovariohysterectomy on lipid peroxidation and levels of some antioxidants and biochemical parameters in bitches. Bull Vet Inst Pulawy 55: 695-698.
• Halliwell B (1994) Free radicals and antioxidants: a personal view. Nutr Rev 70: 257-265.
• Halliwell B, Chirico S (1993) Lipid peroxidation: its mechanism, measurement, and significance. Am J Clin Nutr 57: 715S-725S.
• Höglund OV, Olsson K, Hagman R, Öhlund M, Olsson U, Lagerstedt AS (2011) Comparison of haemodynamic changes during two surgical methods for neutering female dogs. Res Vet Sci 91: 159-163.
• John CD, Buckingham JC (2003) Cytokines: regulation of the hypothalamo-pituitary-adrenocortical axis. Curr Opin Pharmacol 3: 78-84.
• Jones CJ, Budsberg SC (2000) Physiologic characteristics and clinical importance of the cyclooxygenase isoforms in dogs and cats. J Am Vet Med Assoc 217: 721-729.
• Kay-Mugford P, Benn SJ, LaMarre J, Conlon P (2000) In vitro effects of nonsteroidal anti-inflammatory drugs on cyclooxygenase activity in dogs. Am J Vet Res 61: 802-810.
• Kim YK, Lee SS, Suh EH, Lee L, Lee HC, Lee HJ, Yeon SC (2012) Sprayed intraperitoneal bupivacaine reduces early postoperative pain behavior and biochemical stress response after laparoscopic ovariohysterectomy in dogs. Vet J 191: 188-192.
• Kishimoto J, Tsuchiya T, Emson PC, Nakayama Y (1996) Immobilization-induced stress activates neuronal nitric oxide synthase (nNOS) mRNA and protein in hypothalamic- pituitary-adrenal axis in rats. Brain Res 720: 159-171.
• Lai FJ, Huang SS, Hsieh MC, Hsin SC, Wu CH, Hsin YC, Shin SJ (2005) Upregulation of neuronal nitric oxide synthase mRNA and protein in adrenal medulla of water-deprived rats. J Histochem Cytochem 53: 45-53.
• Miranda KM, Espey MG, Wink DA (2001) A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite. Nitric Oxide 5: 62-71.
• Mohn CE, Fernandez-Solari J, De Laurentiis A, Prestifilippo JP, de la Cal C, Funk R, Bornstein SR, McCann SM, Rettori V (2005) The rapid release of corticosterone from the adrenal induced by ACTH is mediated by nitric oxide acting by prostaglandin E2. Proc Natl Acad Sci USA 102: 6213-6218.
• Mollace V, Muscoli C, Masini E, Cuzzocrea S, Salvemini D (2005) Modulation of prostaglandin biosynthesis by nitric oxide and nitric oxide donors. Pharmacol Rev 57: 217-252.
• Monau TR, Vargas VE, King N, Yellon SM, Myers DA, Ducsay CA (2009) Long-term hypoxia increases endothelial nitric oxide synthase expression in the ovine fetal adrenal. Reprod Sci 16: 865-874.
• Naziroglu M, Gunay C (1999) The levels of some antioxidant vitamins, glutathione peroxidase and lipoperoxidase during the anaesthesia of dogs. Cell Biochem Funct 17: 207-212.
• Pachk K, Palkovits M (2001) Stressor specificity of central neuroendocrine responses: implications for stress-related disorders. Endocr Rev 22: 502-548.
• Palmer RM, Ashton DS, Moncada S (1988) Vascular endothelial cells synthesize nitric oxide from L-arginine. Nature 333: 664-666.
• Rettori V, Fernandez-Solari J, Mohn C, Zorrilla Zubilete MA, de la Cal C, Prestifilippo JP, De Laurentiis A (2009) Nitric oxide at the crossroad of immunoneuroendocrine interactions. Ann NY Acad Sci 1153: 35-47.
• Sainz JM, Reche C, Räbano MA, Mondillo C, Patrignani ZJ, Macarulla JM, Pignataro OP, Trueba M (2004) Effects of nitric oxide on aldosterone synthesis and nitric oxide synthase activity in glomerulosa cells from bovine adrenal gland. Endocrine 24: 61-71.
• Serin G, Kiral F, Serin I (2008) Acute effect of ovariohysterectomy on lipid peroxidation and some antioxidant levels in dogs. Bull Vet Inst Pulawy 52: 251-253.
• Wang H, Walker SW, Mason JI, Morley SD, Williams BC (2000) Role of arachidonic acid metabolism in ACTH-stimulated cortisol secretion by bovine adrenocortical cells. Endocr Res 26: 705-709.
• Yoshioka T, Kawada K, Shimada T, Mori M (1979) Lipid peroxidation in maternal and cord blood and protective mechanism against activated-oxygen toxicity in the blood. Am J Obstet Gynecol 135: 372-376.

Identyfikatory

DOI

10.2478/pjvs-2014-0071