Evaluation of genetic variability within sweet cherry (Prunus avium L.) genetic resources by molecular SSR marker

• Autorzy: Patrzak J. , Henychova A. , Paprstein F. , Sedlak J.
• Języki publikacji: EN

Abstrakty

EN

Sweet cherry is a vegetatively propagated, perennial plant with high level of heterozygosity and ancient breeding history. Therefore, it is necessary to keep, conserve and evaluate known genetic resources for future breeding programs and fruit production stability. In present, the utilization of DNA molecular genetic analyses is the best suitable method for evaluation of individual accessions, thus we eliminated duplications and characterized the genetic relationships. In our work, we used PCR primer combinations for 19 SSR and 2 EST-SSR loci for analyses of 123 current, old and local sweet cherry cultivars from Czech genetic resources of Research and Breeding Institute of Pomology in Holovousy. In total, 115 polymorphic fragments were amplified, which we used for hierarchical cluster analysis of genetic variability. The result dendrograms were divided into three main clusters and ten subgroups. Clustering corresponded to genealogical and geobotanical characteristics of individual accessions as breeding history of several known accessions.

Słowa kluczowe

EN

Czech Republic; plant cultivation; sweet cherry; Prunus avium; fruit tree; genetic variability; genetic evaluation; genetic resource; molecular marker; SSR molecular marker; EST-SSR marker

• Wydawca: -
• Czasopismo: Acta Scientiarum Polonorum. Hortorum Cultus
• Rocznik: 2019
• Tom: 18
• Numer: 3
• Opis fizyczny: p.157-165,fig.,ref.

Twórcy

autor

Patrzak J.

• Hop Research Institute, Zatec, Czech Republic

autor

Henychova A.

• Hop Research Institute, Zatec, Czech Republic

autor

Paprstein F.

• Research and Breeding Institute of Pomology, Holovousy, Czech Republic

autor

Sedlak J.

• Research and Breeding Institute of Pomology, Holovousy, Czech Republic

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Identyfikatory

DOI

10.24326/asphc.2019.3.15