PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2014 | 36 | 01 |

Tytuł artykułu

Micropropagation of Cymbidium sinense using continuous and temporary airlift bioreactor system

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Airlift bioreactors were programmed for continuous and temporary immersion culture to investigate factors that affect the rhizome proliferation, shoot formation, and plantlet regeneration of Cymbidium sinense. During rhizome proliferation, the continuous immersion bioreactor system was used to explore the effects of activated charcoal (AC) in the culture medium, inoculation density, and air volume on rhizome differentiation and growth. The optimum conditions for obtaining massive health rhizomes were 0.3 g l⁻¹ AC in the culture medium, 7.5 g l⁻¹ inoculation density, and 150 ml min⁻¹ air. In addition, the temporary immersion bioreactor system was used for both shoot formation and plantlet regeneration. Supplementing 4 mg l⁻¹ 6-benzylaminopurine and 0.2 mg l⁻¹ naphthalene acetic acid (NAA) to the culture medium promoted shoot induction from the rhizome. Cutting the rhizome explants into 1 cm segments was better for massive shoot formation than cutting into 0.25 and 0.5 cm explant segments. NAA promoted plantlet regeneration and the rooting rate (94.7 %), with whole plantlets growing well in culture medium containing 1.0 mg l⁻¹ NAA. Therefore, applying bioreactors in C. sinense micropropagation is an efficient way for scaling up the production of propagules and whole plantlets for the industrial production of high-quality seedlings.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

36

Numer

01

Opis fizyczny

p.117-124,fig.,ref.

Twórcy

autor
  • Key Laboratory of Nature Resource of Changbai Mountain and Functional Molecular, Yanbian University, Ministry of Education, Park Road 977, Yanji 133002, Jilian, China
autor
  • Key Laboratory of Nature Resource of Changbai Mountain and Functional Molecular, Yanbian University, Ministry of Education, Park Road 977, Yanji 133002, Jilian, China
autor
  • Key Laboratory of Nature Resource of Changbai Mountain and Functional Molecular, Yanbian University, Ministry of Education, Park Road 977, Yanji 133002, Jilian, China
autor
  • Key Laboratory of Nature Resource of Changbai Mountain and Functional Molecular, Yanbian University, Ministry of Education, Park Road 977, Yanji 133002, Jilian, China
autor
  • Department of Herticulture Science, Chungbuk National University, Cheong-ju 361-763, Republic of Korea

Bibliografia

  • Ahmed S, Hahn EJ, Paek KY (2008) Aeration volume and photosynthetic photon flux affect cell growth and secondary metabolite contents in bioreactor cultures of Morinda citrifolia. J Plant Biol 51:209–212.
  • Aktar S, Nasiruddin KM, Huq H (2007) In vitro root formation in Dendrobium orchid plantlets with IBA. J Agr Rural Dev 5:48–51.
  • Chang C, Chang WC (2000) Effect of thidiazuron on bud development of Cymbidium sinense Willd in vitro. Plant Growth Regul 30:171–175.
  • Christos C, Chrysovalantou A, Ioannis P, Ioannis T, Kortessa D (2010) Effects of NAA and vitamin B2 on in vitro rooting of Citrus. Acta Agr Scand B-S P 60:189–192.
  • Chugh S, Guha S, Rao IU (2009) Micropropagation of orchids: a review on the potential of different explants. Sci Hortic 122:507–520.
  • Cui XH, Murthy HN, Jin YX, Yim YH, Kim JY, Paek KY (2011) Production of adventitious root biomass and secondary metabolites of Hypericum perforatum L. in a balloon type airlift reactor. Bioresource Technol 102:10072–10079.
  • Hahlbrock K, Kuhlen E (1972) Relationship between growth of parsley and soybean cells in suspension cultures and changes in the conductivity of the culture medium. Planta 108:271–278.
  • Hahn EJ, Paek KY (2005) Multiplication of Chrysanthemum shoots in bioreactors as affected by culture method and inoculation density of single node stems. Plant Cell Tissue Org Cult 81:301–306.
  • Hasegawa A, Ohashi H, Goi M (1985) Effects of BA, rhizome length, mechanical treatment and liquid sharking culture on the shoot formation from rhizome in Cymbidium faberi Rolfe. Acta Hortic 166:25–40.
  • Hessami S, Babaei A (2012) Banana micropropagation using an exclusive temporary immersion bioreactor. In Vitro Cell Dev Biol An 48:77.
  • Huang TK, McDonald KA (2012) Bioreactor systems for in vitro production of foreign proteins using plant cell cultures. Biotechnol Adv 30:398–409.
  • Jeong CS, Chakrabarty D, Hahn EJ, Lee HL, Paek KY (2006) Effects of oxygen, carbon dioxide and ethylene on growth and bioactive compound production in bioreactor culture of ginseng adventitious roots. Biochem Eng J 27:252–263.
  • Jin H, Piao XC, Sun D, Xiu JR, Lian ML (2007) Mass production of rhizome and shoot of Cymbidum niveo-maginatum using simple bioreactor. J Northeast Forest Univ 35:44–48.
  • Kakani A, Li GS, Peng ZH (2009) Role of AUX1 in the control of organ identity during in vitro organogenesis and in mediating tissue specific auxin and cytokinin interaction in Arabidopsis. Planta 229:645–657.
  • Kano K (1965) Studies on the media for orchid seed germination. Mem Fac Agric Kagawa Univ 20:1–68.
  • Kim EK, Hahn EJ, Murthy HN, Paek KY (2004) Enhanced shoot and bulblet proliferation of garlic (Album sativum L.) in bioreactor systems. J Hortic Sci Biotech 79:818–822.
  • Lee OR, Yang DC, Chung HJ, Min BH (2011) Efficient in vitro plant regeneration from hybrid rhizomes of Cymbidium sinense seeds. Hortic Environ Biotechnol 52:303–308.
  • Lian ML, Chakrabarty D, Paek KY (2003) Growth of Lilium oriental hybrid ‘Casablanca’ bulblet using bioreactor culture. Sci Hortic 97:41–48.
  • Lu I, Sutter E, Burger D (2001) Relationships between benzyladenine uptake, endogenous free IAA levels and peroxidase activities during upright shoot induction of Cymbidium ensifoilum cv. Yuh Hwa rhizomes in vitro. Plant Growth Regul 35:161–170.
  • Melekber S, Aysun C (2013) Micropropagation of cherry laurel Prunus laurocerasus L. Food Agric Environ 11:576–579.
  • Mohsen KHE (2001) Comparison, determination and optimizing the conditions required for rhizome and shoot formation, and flowering of in vitro cultured calla explants. Sci Hortic 101:305–313.
  • Paek KY, Kozai T (1998) Micropropagation of temperate Cymbidium via rhizome culture. HortTechnology 8:283–288.
  • Paek KY, Yeung EC (1991) The effects of 1-naphthaleneacetic acid and N6-benzyladenine on the growth of Cymbidium forrestii rhizomes in vitro. Plant Cell Tissue Org Cult 24:65–71.
  • Park SY, Murthy HN, Paek KY (2000) Mass multiplication of protocorm-like bodies using bioreactor system and subsequent plant regeneration in Phalaenopsis. Plant Cell Tissue Org Cult 63:67–72.
  • Piao XC, Chakrabarty D, Hahn EJ, Paek KY (2003) A simple method for mass production of potato microtubers using a bioreactor system. Curr Sci India 84:1129–1132.
  • Schlatmann JE, Moreno PRH, Vinke JL, Tenhoopen HJG, Verpoorte R, Heijnen JJ (1994) Effect of oxygen and nutrient limitation on ajmalicine production and related enzyme-activities in high-density cultures of Catharanthus roseus. Biotechnol Bioeng 44:461–468.
  • Shiau YJ, Nalawade SM, Hsai CN, Tsay HS (2005) Propagation of Haemaria discolor via in vitro seed germination. Biol Plantarum 49:341–346.
  • Shimasaki K, Uemoto S (1990) Micropropagation of a terrestrial Cymbidium species using rhizomes developed from seeds and pseudobulbs. Plant Cell Tissue Org Cult 23:237–244.
  • Singh S, Ray BK, Bhattacharyya S, Deka CP (1994) In vitro propagation of Citrus reticulata Blanco and Citrus limon Burm. f. HortScience 29:214–216.
  • Snyman SJ, Meyer GM, Richards JR, Ramgareeb S, Banasiak M, Huckett B (2007) Use of the temporary immersion RITA (R) bioreactor system for micropropagation of sugarcane. S Afr J Bot 73:336–337.
  • Srivastava S, Srivastava AK (2012) In vitro azadirachtin production by hairy root cultivation of Azadirachta indica in nutrient mist bioreactor. Appl Biochem Biotech 166:365–378.
  • Thompson DI, Edwards TJ, van Staden J (2006) Evaluating asymbiotic seed culture methods and establishing Disa (Orchidaceae) germinability in vitro: relationships, requirements and first-time reports. Plant Growth Regul 49:269–284.
  • Wang GR, Qi NM (2010) Influence of mist intervals and aeration rate on growth and second metabolite production of Pseudostellaria heterophylla adventitious roots in a siphon-mist Bioreactor. Biotechnol Bioproc E 15:1059–1064.
  • Wang SM, Piao XC, Park SY, Lian ML (2012) Improved micropropagation of Gypsophila paniculata with bioreactor and factors affecting ex vitro rooting in microponic system. In Vitro Cell Dev Biol Plant 49:70–78.
  • Watt MP (2012) The status of temporary immersion system (TIS) technology for plant micropropagation. Afr J Biotechnol 11:4025–14035.
  • Weng JZ, Lin JG, Lin JB (2006) Influence of different activated carbon concentrations on culture in vitro of Cymbidium sinense. J Trop Subtrop Bot 35:37–38.
  • Wu RZ, Chakrabarty D, Hahn EJ, Paek KY (2007) Micropropagation of an endangered jewel orchid (Anoectochilus formosanus) using bioreactor system. Hortic Environ Biotechnol 48:376–380.
  • Yan N, Hu H, Huang J, Xu K, Wang H, Zhou Z (2006) Micropropagation of Cypripedium flavum through multiple shoots of seedlings derived from mature seeds. Plant Cell Tissue Org Cult 84:114–118.
  • Yang JF, Piao XC, Sun D, Lian ML (2010) Production of protocormlike bodies with bioreactor and regeneration in vitro of Oncidium ‘Sugar Sweet’. Sci Hortic 125:712–717.
  • Yoon YJ, Murthy HN, Hahn EJ, Paek KY (2007) Biomass production of Anoectochilus formosanus Hayata in a bioreactor system. J Plant Biol 50:573–576.
  • Zhang CH, Piao XC, Lian ML, Wang SM (2007) Application of bioreactors in rapid propagation of Gypsophila paniculata. Chinese Bull Bot 24:526–531.
  • Zhao Y, Sun W, Wang Y, Saxena PK, Liu CZ (2012) Improved mass multiplication of Rhodiola crenulata shoots using temporary immersion bioreactor with forced ventilation. Appl Biochem Biotech 166:1480–1490.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-262c7f16-1b3e-4d7d-b68d-4562c1bcab53
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.