Micropropagation of Rehmannia glutinosa Libosch.: production of phenolics and flavonoids and evaluation of antioxidant activity

• Autorzy: Piatczak E. , Grzegorczyk-Karolak I. , Wysokinska H.
• Języki publikacji: EN

Abstrakty

EN

Rehmannia glutinosa Libosch., a valuable medicinal plant, was successfully propagated in vitro using shoot tip explants. Shoot multiplication was performed in glass tubes and in a nutrient sprinkle bioreactor. A mixture of 0.1 mg L⁻¹ indole-3-acetic acid (IAA) and 1.0 mg L⁻¹ of 6-benzylaminopurine in Murashige and Skoog (MS) agar-solidified medium proved the best combination for multiple shoot induction, yielding 8.2 shoots per explant after 4 weeks of culture in glass tubes. The number of shoots increased to 21 per explant when the same combination of growth regulators was used in a nutrient sprinkle bioreactor. The shoots rooted with a frequency of 93 % after 6 weeks of culture on MS agar medium supplemented with IAA (0.1 mg L⁻¹) before being acclimatized in the greenhouse. The antioxidant activities of methanolic extracts from the leaves and roots of the in vitro-regenerated plants of R. glutinosa cultivated in the greenhouse were evaluated using four in vitro assays: scavenging of free radicals (DPPH and ABTS), transition metal reduction and total antioxidant activity phosphomolybdenum test. In all cases, the methanolic extract from leaves demonstrated better antioxidant activity than those taken from roots. A strong correlation was found between total phenolic and flavonoid content, and the antioxidant capacity of the studied extracts.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2014
• Tom: 36
• Numer: 07
• Opis fizyczny: p.1693-1702,fig.,ref.

Twórcy

autor

Piatczak E.

• Department of Biology and Pharmaceutical Botany, Medical University of Lodz, Muszynskiego 1, 90-151 Lodz, Poland

autor

Grzegorczyk-Karolak I.

• Department of Biology and Pharmaceutical Botany, Medical University of Lodz, Muszynskiego 1, 90-151 Lodz, Poland

autor

Wysokinska H.

• Department of Biology and Pharmaceutical Botany, Medical University of Lodz, Muszynskiego 1, 90-151 Lodz, Poland

Bibliografia

• Anh NTH, Sung TV, Fronke K, Wessjohann LA (2003) Phytochemical studies of Rehmannia glutinosa rhizomes. Pharmazie 58:593–595
• Aremu AO, Gruz J, Šubrtová M, Szučová L, Doležal K, Bairu MW, Finnie JF, van Staden J (2013) Antioxidant and phenolic acid profiles of tissue cultured and acclimatized Merwilla plumbea plantlets in relation to the applied cytokinins. J Plant Physiol 170:1303–1308. doi:10.1007/s11738-012-1027-6
• Cai Y, Luo Q, Sun M, Corke H (2004) Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer. Life Sci 74:2157–2184. doi:10.1016/j.lfs.2003.09.047
• Chung IM, Kim JJ, Lim JD, Yu ChY, Kim SH, Hahn SJ (2006) Comparison of resveratrol, SOD activity, phenolic compounds and free amino acids in Rehmannia glutinosa under temperature and water stress. Environ Exp Bot 56:44–53. doi:10.1016/j.envexpbot.2005.01.001
• Es-Safi N-E, Kollmann A, Khlifi S, Ducrot P-H (2007) Antioxidative effect of compounds isolated from Globularia alypum L. structure–activity relationship. LWT 40:1246–1252. doi:10.1016/j.lwt.2006.08.019
• Hatano M, Nakai R, Kawanishi F, Kedo K, Shoyama Y (1997) Genetic diagnosis of Rehmannia species micropropagated by tip tissue culture and an F1 hybrid by RAPD analysis. Plant Breed 116:589–591
• Jahan AA, Anis M (2009) In vitro rapid multiplication and propagation of Cardiospermum halicacabum L. through axillary bud culture. Acta Physiol Plant 31:133–138. doi:10.1007/s11738-008-0211-1
• Jeong JH, Yu KW, Chakrabarty D, Kim SJ, Peak KY (2002) In vitro regeneration and plantlet formation from adventitious roots of R. glutinosa Liboschits. Propag Ornam Plants 2:19–23
• Kirby A, Schmidt RJ (1997) The antioxidant activity of Chinese herbs for eczema and of placebo herbs: I. J Ethnopharmacol 56:103–108
• Kitagawa I, Fukuda Y, Taniyama T, Yoshikawa M (1991) Chemical studies on crude drug processing. VII. On the constituents of Rehmanniae radix. (1): absolute stereostructures of rehmaglutins A, B and D isolated from Chinese Rehmanniae Radix, the dried root of R. glutinosa Libosch. Chem Pharm Bull 39:1171–1176
• Konstas J, Kintzios S (2003) Developing a scale-up system for the micropropagation of cucumber (Cucumis sativus L.): the effect of growth retardants, liquid culture and vessel size. Plant Cell Rep 21:538–548. doi:10.1007/s00299-002-0566-5
• Lamaison JL, Carnat A (1990) Content of principal flawonoids of the flowers and leaves of Crataegus monogyna Jacq. and Crataegus laeviagata (Poiret) D.C. Rosaceae. Pharm Acta Helv 65:315–320
• Li HB, Wong CC, Cheng KW, Chen F (2008) Antioxidant properties in vitro and total phenolic contents in methanol extracts from medicinal plants. LWT 41:385–390. doi:10.1016/j.lwt.2007.03.011
• Mark TR, Simpson SE (1994) Factor affecting shoots development in apically dominant Acer cultivars in vitro. J Hortic Sci 69:543–551
• Matkowski A (2008) Plant in vitro culture for the production of antioxidants––a review. Biotechnol Adv 26(548):560. doi:10.1016/j.biotechadv.2008.07.001
• Matsumoto M, Shoyama Y, Nishioka I, Irino N (1989) Constituents of regenerated and shoot cultured root tissue of R. glutinosa. Phytochemistry 28:2331–2332
• Meszaros A, Bellon A, Pinter E, Horvath G (1999) Micropropagation of lemon balm. Plant Cell Tiss Org Cult 57:149–152
• Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco culture. Physiol Plant 15:473–497
• Ncube B, Ngunge VNP, Finnie JF, Van Staden J (2011) A comparative study of the antimicrobial and phytochemical properties between outdoor grown and micropropagated Tulbaghia violacea Harv. plants. J Ethnopharmacol 134:775–780
• Paek KY, Yu KJ, Park SI, Sung NS, Park CH (1995) Micropropagation of R. glutinosa as medicinal plant by shoot tip and root segment culture. ISHS international symposium on medicinal and aromatic plants. Acta Hortic 390:120–123
• Park SU, Kim YK, Lee SY (2009) Improved in vitro plant regeneration and micropropagation of R. glutinosa L. J Med Plants Res 3:31–34
• Pharmacopoeia Commission of the People’s Republic of China (2000) The pharmacopoeia of the People’s Republic of China, vol 1. Chemical Industry Publishing House, Beijing, p 94
• Piątczak E, Chmiel A, Wysokińska H (2005) Mist trickling bioreactor for Centaurium erythraea Rafn growth of shoots and production of secoiridoids. Biotechnol Lett 27:721–724. doi:10.1007/s10529-005-5189-9
• Piątczak E, Królicka A, Wielanek M, Wysokińska H (2012) Hairy root cultures of R. glutinosa and the production of iridoid and phenylethanoid glycosides. Acta Physiol Plant 34:2215–2224. doi:10.1007/s11738-012-1022-y
• Prieto P, Pineda M, Aguilar M (1999) Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Anal Biochem 269:337–341
• Pulido R, Bravo L, Saura-Calixto F (2000) Antioxidant activity of dietary polyphenols as determined by a modified ferric reducing antioxidant power assay. J Agricult Food Chem 48:3396–3402. doi:10.1021/jf9913458
• Re RP, Pellegrini A, Pannala A, Yang M, Rice-Evans C (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 26:1231–1237
• Sesterhenn K, Distl M, Wink M (2007) Occurrence of iridoid glycosides in in vitro cultures and intact plants of Scrophularia nodosa L. Plant Cell Rep 26:365–371. doi:10.1007/s00299-006-0233-3
• Shaik S, Dewir YH, Singh N, Nicholas A (2010) Micropropagation and bioreactor studies of the medicinally important plant Lessertia (Sutherlandia) frutescens. South Afr J Bot 76:180–186. doi:10.1016/j.sajb.2009.10.005
• Shoyama Y, Nagano M, Nishioka I (1983) Clonal multiplication of R. glutinosa. Planta Med 48:124–128
• Singleton V, Rossi JA (1965) Colorimetry of total phenolics with phosphomolybdic–phosphotungstic acid reagents. Am J Enol Viticult 16:144–158
• Słupski W, Tubek B, Matkowski A (2011) Micropropagation of Codonopsis pilosula (Franch.) Nannf. by axillary shoot multiplication. Acta Biol Crac Series Botanica 53/2:87–93. doi:10.2478/v10182-011-0031-2
• Sudha CG, Krishnan PN, Pushpangadan P, Seeni S (2005) In vitro propagation of Decelepis arayalpathra, a critically endangered ethnomedicinal plant. In Vitro Cell Dev Biol Plant 41:648–654. doi:10.1079/IVP2005652
• Weremczuk-Jeżyna I, Grzegorczyk-Karolak I, Frydrych B, Królicka A, Wysokińska H (2013) Hairy roots of Dracocephalum moldavica: Rosmarinic acid content and antioxidant potential. Acta Physiol Plant 35:2095–2103. doi:10.1007/s11738-013-1244-7
• Wong CC, Li HB, Cheng KW, Chen F (2006) A systematic survey of antioxidant activity of 30 Chinese medicinal plants using the ferric reducing antioxidant power assay. Food Chem 97:705–711. doi:10.1016/j.foodchem.2005.05.049
• Wu M, Wu P, Liu M, Xie H, Jiang Y, Wei X (2009) Iridoids in Gentiana loureirii. Phytochemistry 70:746–750. doi:10.1016/j.phytochem.2009.03.018
• Xu XH, Davey MR (1983) Shoot regeneration from mesophyll protoplasts and leaf explants of R. glutinosa. Plant Cell Rep 2:55–57
• Xuesen W, Delan H, Xiaoju M, Xianen L, Junhua Z (2002) Multiplication of virus-free seedlings of R. glutinosa. Zhongcaoyao 33:452–455
• Zhang RX, Li MX, Jia ZP (2008) Rehmannia glutinosa: review of botany, chemistry and pharmacology. J Ethnopharm 117:199–214. doi:10.1016/j.jep.2008.02.018
• Zhao H, Tan J, Qi Ch (2007) Photosynthesis of R. glutinosa subjected to drought stress is enhanced by choline chloride through alleviating lipid peroxidation and increasing proline accumulation. Plant Growth Reg 51:255–262. doi:10.1007/s10725-007-9167-1
• Zhenchen Z, Qi Q, Xiulan J, Yongijang W (2004) The techniques and application of virus-free R. glutinosa. Zhiwu Boahu Xuebao 31:342–346
• Zhou Y, Gu F, Zhou Ch, Yao H, Duan H, Wang F, Liu Y, Xing Y, Chu S (2010) Genetic diversity of R. glutinosa cultivars based on sequence-related amplified polymorphism markers. Sci Hort 125:789–794. doi:10.1016/j.scienta.2010.05.02

Identyfikatory

DOI

10.1007/s11738-014-1544-6