Sequence analysis of the ORF7 region of chinese transmissible gastroenteritis virus isolate TGEs-1

• Autorzy: Lan D. , Gu J. , Xing R. , Yuan C. , Cui L. , Hua X. , Yang Z.
• Języki publikacji: EN

Abstrakty

EN

Transmissible gastroenteritis virus (TGEV) was isolated from pigs suspected of having transmissible gastroenteritis (TGE) in China. The open reading frame 7 (ORF 7) of this isolate, designated as TGEs-1, which is located at the 3' end of the TGEV genome, was amplified by the RT-PCR. The amplified PCR product was cloned, sequenced, and compared with the sequences of 24 TGEV reference strains. TGEs-1 strain had 96.2%-100% nucleotide and 93.8%-100% amino acid sequence similarity with the other Chinese TGEV strains, and 92.4%-100% nucleotide and 92.5%-100% amino acid sequence similarity with non-Chinese TGEV strains. Interestingly, three virulent strains: TGEs-1, TS, and Miller showed a complete homology with H16 and H attenuated virulent strains in the nucleotide and amino acid sequences of ORF 7. In phylogenetic analysis of the ORF 7 gene, Chinese TGEV strains were clustered into three groups. TGEs-1 with three Chinese strains (TS, H16, and attenuated H) and one American strain (Miller) were clustered into single group. It should be noted that the Chinese and Asian TGEV strains were related to the TGEV strains isolated in the USA, and alien to the European strains.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2011
• Tom: 55
• Numer: 2
• Opis fizyczny: p.169-175,fig.,ref.

Twórcy

autor

Lan D.

• Shanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China

autor

Gu J.

autor

Xing R.

autor

Yuan C.

autor

Cui L.

autor

Hua X.

autor

Yang Z.

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