EN
Propyl 4-(2-(4,6-dimethoxypyrimidin-2-yloxy) benzylamino)benzoate (ZJ0273) is a new herbicide which inhibits acetolactate synthase (ALS). The ZJ0273 is considered as safe for the environment and exhibits a satisfactory effect on weed control in the rapeseed field. ALS is the key enzyme of reactions in the biosynthesis of total amino acids (TAAs) especially branched-chain amino acids (BCAAs). This study reports the effect of ZJ0273 on BCAAs and TAAs in rapeseed leaves using near-infrared spectroscopy (NIRS) techniques. A decrease in TAAs and BCAAs contents was observed as the herbicide dosages were increased along with leaf senescence. The wavelengths 2,416 and 1,340 nm were selected to develop the NIRS model for detecting BCAAs and TAAs, and correlation coefficients of model’s prediction set were 0.9823, 0.9764, 0.9831, and 0.9968 for valine, isoleucine, leucine, and TAAs, respectively. The results indicated that 100 mg/L ZJ0273 was a safe dosage for oilseed rape as it did not show a significant effect on the contents of amino acids compared to other higher dosages (500 and 1,000 mg/L).