Optimization and production of alkaline proteases from agro byproducts using a novel Trichoderma viridiae strain VPG 12, isolated from agro soil

• Autorzy: Shivasharanappa K. , Hanchinalmath J. V. , Sundeep Y. S. , Borah D. , Prasad Talluri V. S. S. L.
• Języki publikacji: EN

Abstrakty

EN

In recent years, there has been a phenomenal increase in the use of alkaline proteases as industrial catalysts. The aim of this work was to isolate potent fungal strain from the agricultural field of Gulbarga region of India, for the production of alkaline protease by utilizing the agricultural by products viz, red and green gram and Bengal gram as substrate under submerged fermentation process. Optimization of fermentation process parameters such as substrate (Red gram husk, green gram husk and Bengal gram husk) utilization, utilization, temperature, pH and incubation period for alkaline protease production was carried out. The maximum production of alkaline protease by Trichoderma VPG 12 was found at pH 8, temperature 35 °C, incubated for 120 h. But the activity of the enzyme could also be seen in a wide range of pH (5-9) and temperature (20-40 °C). With all these properties, the strain can be considered for industrial grade production of alkaline protease.

Słowa kluczowe

EN

optimization; production; alkaline protease; agricultural product; Trichoderma viride; agricultural soil; fermentation process; red gram husk; substrate

• Wydawca: -
• Czasopismo: International Letters of Natural Sciences
• Rocznik: 2014
• Tom: 09
• Opis fizyczny: p.77-84,fig.,ref.

Twórcy

autor

Shivasharanappa K.

• Department of Biotechnology, Gulbarga University, Gulbarga - 585106, Karnataka, India

autor

Hanchinalmath J. V.

• Department of Biotechnology, Gulbarga University, Gulbarga - 585106, Karnataka, India

autor

Sundeep Y. S.

• Department of Botany, Sikkim Central University, Gangtok - 737102, Sikkim, India

autor

Borah D.

• Centre for Studies in Biotechnology, Dibrugarh University, Dibrugarh - 786004, India

autor

Prasad Talluri V. S. S. L.

• Department of Biotechnology, GITAM Institute of Science, GITAM University, Vishakhapatnam - 530045, India

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