Detection of rabies antibodies in dog sera

• Autorzy: Ondrejkova A. , Suli J. , Ondrejka R. , Slepecka E. , Prokes M. , Cechvala P. , Supuka P.
• Języki publikacji: EN

Abstrakty

EN

In the presented work, we compared the results of determination of rabies antibodies using three in vitro methods: rapid fluorescent focus inhibition test (RFFIT), fluorescent antibody virus neutralisation test (FAVNT) and the immunoenzymatic assay (ELISA). 196 dog sera samples were examined with FAVNT, RFFIT methods and the ELISA test. Sera with low and sufficiently high titre of antibodies had a similar result in determining by all methods. A critical level of rabies antibodies close to the required protection level (0.5 IU/cm3) was seen in sera of 18 dogs (9.18%); these were the sera obtained after primary vaccination of dogs. At this level, even small differences can cause a change in the assessment of the patient's serum seronegativity or seropositivity. Therefore, it is important to choose the appropriate method that has sufficiently strict criteria while having a good reproducibility.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2015
• Tom: 18
• Numer: 1
• Opis fizyczny: p.47-51,fig.,ref.

Twórcy

autor

Ondrejkova A.

• Department of Epizootiology and Parasitology, University of Veterinary Medicine and Pharmacy in Kosice, Komenskeho 73, 041 81 Kosice, Slovak Republic

autor

Suli J.

• Department of Chemistry, Biochemistry and Biophysics, University of Veterinary Medicine and Pharmacy in Kosice, Komenskeho 73, 041 81 Kosice, Slovak Republic

autor

Ondrejka R.

• Department of Epizootiology and Parasitology, University of Veterinary Medicine and Pharmacy in Kosice, Komenskeho 73, 041 81 Kosice, Slovak Republic

autor

Slepecka E.

• Department of Epizootiology and Parasitology, University of Veterinary Medicine and Pharmacy in Kosice, Komenskeho 73, 041 81 Kosice, Slovak RepublicDepartment of Epizootiology and Parasitology, University of Veterinary Medicine and Pharmacy in Kosice, Komenskeho 73, 041 81 Kosice, Slovak Republic

autor

Prokes M.

autor

Cechvala P.

• Department of Epizootiology and Parasitology, University of Veterinary Medicine and Pharmacy in Kosice, Komenskeho 73, 041 81 Kosice, Slovak Republic

autor

Supuka P.

• Department of Nutrition, Dietetics and Animal Husbandry, University of Veterinary Medicine and Pharmacy in Kosice, Komenskeho 73, 041 81 Kosice, Slovak Republic

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Identyfikatory

DOI

10.1515/pjvs-2015-000